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Determination of Terbium's Oxidative Stress and Antioxidant Responses in Pontastacus leptodactylus

Year 2024, Volume: 20 Issue: 1, 23 - 32, 01.03.2024
https://doi.org/10.22392/actaquatr.1294250

Abstract

Terbium (Tb) is a rare earth element (REE) that can become toxic to aquatic organisms when present in high concentrations in the aquatic environment. There are still not enough studies on the oxidative stress consequences of Tb toxicity in the freshwater ecosystem. In this study, the freshwater creature Pontastacus leptodactylus was used to evaluate the oxidative stress consequences of Tb. The oxidative stress and antioxidant changes caused by Tb at 1, 5 and 25 mg/L concentrations at 24 and 96 hours in P. leptodactylus, were affected by the enzyme activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and thiobarbituric acid (TBARS) and reduced levels of glutathione (GSH) were determined. Results were determined using ELISA kits. SPSS 24.0 package program one-way ANOVA (Duncan 0.05) test was used in the statistical evaluation of biomarker analyses. While no significant change was observed in SOD activity (p>0.05) compared to the control group, significant decreases were observed in CAT and GPx activities (p<0.05); Significant (p<0.05) increases were observed in GSH and TBARS levels. Considering the study data, it is concluded that the TB xenobiotic that penetrates the body of living organisms causes oxidative stress in the organism.

References

  • Adeel, M., Lee, J. Y., Zain, M., Rizwan, M., Nawab, A., Ahmad, M. A., ... and Xing, B. (2019). Cryptic footprints of rare earth elements on natural resources and living organisms. Environment international, 127, 785-800.
  • Amato, J., Morigi, R., Pagano, B., Pagano, A., Ohnmacht, S., De Magis, A., ... and Randazzo, A. (2016). Spesifik G-dörtlü bağlayıcıların geliştirilmesine doğru: Yeni hidrazon türevlerinin sentezi, biyofiziksel ve biyolojik çalışmaları. Tıbbi kimya dergisi, 59 (12), 5706-5720.
  • Andrade, M., Soares, A. M., Solé, M., Pereira, E., and Freitas, R. (2022). Do climate change related factors modify the response of Mytilus galloprovincialis to lanthanum? The case of temperature rise. Chemosphere, 307, 135577.
  • Andrade, M., Soares, A. M., Solé, M., Pereira, E., and Freitas, R. (2023). Threats of Pollutants Derived from Electronic Waste to Marine Bivalves: The Case of the Rare‐Earth Element Yttrium. Environmental Toxicology and Chemistry, 42(1), 166-177.
  • Bainy, A.C.D., Arisi, A.C.M., Azzalis, L.A., Simizu, K., Barros, S.B.M., Videla, L.A. and Junqueira, V.B.C. (1993). Differential effects of short-term lindane administration on parameters related to oxidative stress in rat liver and erythrocytes. Journal of Biochemical Toxicology, 8(4),187-194.
  • Ballesteros, M.L., Wunderlin, D.A. and Bistoni, M.A. (2009). Oxidative stress responses in different organs of Jenynsia multidentata exposed to endosulfan. Ecotoxicology and Environmental Safety, 72(1),199-205.
  • Barbosa, H., Soares, A. M., Pereira, E., and Freitas, R. (2023). Lithium: A review on concentrations and impacts in marine and coastal systems. Science of The Total Environment, 857, 159374.
  • Charalampides, G., Vatalis, K. I., Apostoplos, B., and Ploutarch-Nikolas, B. (2015). Rare earth elements: industrial applications and economic dependency of Europe. Procedia Economics and Finance, 24, 126-135.
  • Demidchik, V. (2015). Mechanisms of oxidative stress in plants: From classical chemistry to cell biology. Environmental and Experimental Botany, 109: 212- 228.
  • Dimitrova, M.S., Tishinova, V. and Velcheva, V. (1994). Combined effect of zinc and lead on the hepatic superoxide dismutase-catalase system in carp (Cyprinus carpio). Comp Biochem Physiol C: Pharmacol Toxicol Endocrinol, 108(1), 43-46.
  • Durmaz, H., Sevgiler. Y. and Üner, N. (2006). Tissuespecific antioxidative and neurotoxic responses to diazinon in Oreochromis niloticus. Pesticide Biochemistry and Physiology, 84(3), 215-226
  • Freitas, R., Cardoso, C. E., Costa, S., Morais, T., Moleiro, P., Lima, A. F., ... and Pereira, E. (2020). New insights on the impacts of e-waste towards marine bivalves: The case of the rare earth element Dysprosium. Environmental Pollution, 260, 113859.
  • Figueiredo, C., Grilo, TF, Lopes, C., Brito, P., Diniz, M., Caetano, M., ... and Raimundo, J. (2018). Cam yılan balıklarında (Anguilla anguilla) lantan maruziyeti altında birikim, eliminasyon ve nöro-oksidatif hasar. Chemosfer , 206 , 414-423.
  • Galdiero, E., Carotenuto, R., Siciliano, A., Libralato, G., Race, M., Lofrano, G. and Guida, M. (2019). Cerium and erbium effects on Daphnia magna generations: A multiple endpoints approach. Environmental Pollution, 254, 112985.
  • Gobi, N., Vaseeharan, B., Rekha, R., Vijayakumar, S., and Faggio, C. (2018). Bioaccumulation, cytotoxicity and oxidative stress of the acute exposure selenium in Oreochromis mossambicus. Ecotoxicology and environmental safety, 162, 147-159.
  • Hanana, H., Turcotte, P., André, C., Gagnon, C., and Gagné, F. (2017). Comparative study of the effects of gadolinium chloride and gadolinium–based magnetic resonance imaging contrast agent on freshwater mussel, Dreissena polymorpha. Chemosphere, 181, 197-207.
  • Hanana, H., Turcotte, P., Dubé, M., Gagnon, C., and Gagné, F. (2018). Response of the freshwater mussel, Dreissena polymorpha to sub-lethal concentrations of samarium and yttrium after chronic exposure. Ecotoxicology and environmental safety, 165, 662-670.
  • Hanana, H., Taranu, Z. E., Turcotte, P., Gagnon, C., Kowalczyk, J., and Gagné, F. (2021a). Sublethal effects of terbium and praseodymium in juvenile rainbow trout. Science of The Total Environment, 777, 146042.
  • Hanana, H., Taranu, ZE, Turcotte, P., Gagnon, C., Kowalczyk, J., and Gagné, F. (2021b). Nadir toprak elementleri disprosyum ve lutesyuma maruz kalan gökkuşağı alabalığında genel stres, detoksifikasyon yolları ve genotoksisitenin değerlendirilmesi. Ekotoksikoloji ve Çevre Güvenliği, 208, 111588.
  • Hu, Z., Richter, H., Sparovek, G., and Schnug, E. (2004). Physiological and biochemical effects of rare earth elements on plants and their agricultural significance: a review. Journal of plant nutrition, 27(1), 183-220.
  • Lavado, R., Ureña, R., Martin-Skilton, R., Torreblanca, A., Del Ramo, J., Raldua, D., and Porte, C. (2006). The combined use of chemical and biochemical markers to assess water quality along the Ebro River. Environmental Pollution, 139(2), 330-339.
  • Lebrun, J. D., Geffard, O., Urien, N., François, A., Uher, E., and Fechner, L. C. (2015). Seasonal variability and inter-species comparison of metal bioaccumulation in caged gammarids under urban diffuse contamination gradient: implications for biomonitoring investigations. Science of the Total Environment, 511, 501-508.
  • Lompré, J. S., Moleiro, P., De Marchi, L., Soares, A. M., Pretti, C., Chielini, F., and Freitas, R. (2021). Bioaccumulation and ecotoxicological responses of clams exposed to terbium and carbon nanotubes: Comparison between native (Ruditapes decussatus) and invasive (Ruditapes philippinarum) species. Science of The Total Environment, 784, 146914.
  • Migaszewski, Z. M., and Gałuszka, A. (2015). The characteristics, occurrence, and geochemical behavior of rare earth elements in the environment: a review. Critical reviews in environmental science and technology, 45(5), 429-471.
  • Pereira, L.V., Shimizu, M.H., Rodrigues, L.P., Leite, C.C., Andrade, L. and Seguro, A.C. (2012). Nacetylcysteine protects rats with chronic renal failure from gadolinium-chelate nephrotoxicity. Plos One, 7(7), e39528.
  • Pinto, J., Costa, M., Leite, C., Borges, C., Coppola, F., Henriques, B., Monteiro, R., Russo, T., Cosmo, A., Soares, MVN., Polese, G., Pereira, E., Freitas, R., 2019. Ecotoxicological effects of lanthanum in Mytilus galloprovincialis: Biochemical and histopathological impacts, Aquatic Toxicology, 211, 181-192.
  • Ronci, L., Meccoli, L., Iannilli, V., Menegoni, P., De Matthaeis, E., and Setini, A. (2016). Comparison between active and passive biomonitoring strategies for the assessment of genotoxicity and metal bioaccumulation in Echinogammarus veneris (Crustacea: Amphipoda). Italian Journal of Zoology, 83(2), 162-172.
  • Serdar, O., Yıldırım, N., Tatar, Ş., and Yildirim, N. C. (2021). Gadolinyumun Tatlı Su Omurgasızı Dreissena polymorpha Üzerindeki Biyokimyasal Etkileri. International Journal of Pure and Applied Sciences, 7(2), 229-236.
  • Sun, D., He, N., Chen, Q., and Duan, S. (2019). Effects of lanthanum on the photosystem II energy fluxes and antioxidant system of Chlorella Vulgaris and Phaeodactylum Tricornutum. International Journal of Environmental Research and Public Health, 16(12), 2242.
  • Sobjak, T.M., Romão, S., do Nascimento, C.Z., dos Santos, A.F.P., Vogel, L. and Guimarães, A.T.B. (2017). Assessment of the oxidative and neurotoxic effects of glyphosate pesticide on the larvae of Rhamdia quelen fish. Chemospher, 182, 267-275.
  • Tyler, G. (2004). Rare earth elements in soil and plant systems-A review. Plant and soil, 267, 191-206.
  • Tseng, M.T., Lu, X., Duan, X., Hardas, S.S., Sultana, R., Wu, P., Unrine, J.M., Graham, U., Butterfield, D.A., Grulke, E.A. and Yokel, R.A. (2012). Alterationofhepaticstructure and oxidative stress induced by intravenous nanoceria. Toxicology and Applied Pharmacology, 260, 173–182.
  • URL-1: https://evrimagaci.org/terbiyum-10139 (14.04.2023).
  • Van der Oost, R., Beyer, J., and Vermeulen, N. P. (2003). Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environmental toxicology and pharmacology, 13(2), 57-149.
  • Wang, L., Wang, W., Zhou, Q.. and Huang, X. (2014). Combined effects of lanthanum (III) chloride and acid rain on photosynthetic parameters in rice. Chemosphere, 112, 355–361.
  • Wang, L., Zhang, X., Wu, L., Liu, Q., Zhang, D., and Yin, J. (2018). Expression of selenoprotein genes in muscle is crucial for the growth of rainbow trout (Oncorhynchus mykiss) fed diets supplemented with selenium yeast. Aquaculture, 492, 82-90.
  • Zhao, H., Hong, J., Yu, X., Zhao, X., Sheng, L., Ze, Y., Sang, X., Gui, S., Sun, Q., Wang, L.. and Hong, F. (2013). Oxidative stress in the kidney injury of mice following exposure to lanthanides trichloride. Chemosphere, 93, 875–884.

Terbiyumun Pontastacus leptodactylus’ta ki Oksidatif Stres ve Antioksidan Yanıtlarının Belirlenmesi

Year 2024, Volume: 20 Issue: 1, 23 - 32, 01.03.2024
https://doi.org/10.22392/actaquatr.1294250

Abstract

Terbiyum (Tb), su ortamında yüksek konsantrasyonlarda bulunduğunda sucul organizmalar için toksik hale gelebilen nadir toprak elementi (NTE)’dir. Tatlı su ekosistemindeki Tb toksisitesinin meydana getirdiği oksidatif stres sonuçlarıyla ilgili hala yeterince çalışmalar mevcut değildir. Bu çalışma ile Tb'nin oksidatif stres sonuçlarını değerlendirmek için tatlı su canlısı olan Pontastacus leptodactylus kullanılmıştır. Bu organizmada, 1, 5 ve 25 mg/L konsantrasyonlardaki Tb'nin 24 ve 96. saatte meydana getirdiği oksidatif stres ve antioksidan değişimleri süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px), katalaz (CAT) enzim aktiviteleri ile Tiyobarbitürik asit (TBARS) ve redükte glutatyon (GSH) seviyeleri belirlenmiştir. Sonuçlar ELISA kitleri kullanılarak tespit edilmiştir. Biyobelirteç analizlerin istatatistiksel değerlendirilmesinde SPSS 24.0 paket program tek-yönlü ANOVA (Duncan 0,05) testi kullanılmıştır. Kontrol grubu ile karşılaştırıldığında SOD aktivitesinde önemli bir değişim gözlemlenmezken (p>0,05) CAT ve GPx aktivitelerinde anlamlı azalmalar (p<0,05) gözlemlenmiştir; GSH ve TBARS seviyelerinde ise anlamlı (p<0,05) artışlar gözlemlenmiştir. Çalışma verileri dikkate alındığında canlı organizma vücuduna nüfuz eden Tb ksenobiyotiğinin organizma da oksidatif strese neden olduğu düşünülmektedir.

References

  • Adeel, M., Lee, J. Y., Zain, M., Rizwan, M., Nawab, A., Ahmad, M. A., ... and Xing, B. (2019). Cryptic footprints of rare earth elements on natural resources and living organisms. Environment international, 127, 785-800.
  • Amato, J., Morigi, R., Pagano, B., Pagano, A., Ohnmacht, S., De Magis, A., ... and Randazzo, A. (2016). Spesifik G-dörtlü bağlayıcıların geliştirilmesine doğru: Yeni hidrazon türevlerinin sentezi, biyofiziksel ve biyolojik çalışmaları. Tıbbi kimya dergisi, 59 (12), 5706-5720.
  • Andrade, M., Soares, A. M., Solé, M., Pereira, E., and Freitas, R. (2022). Do climate change related factors modify the response of Mytilus galloprovincialis to lanthanum? The case of temperature rise. Chemosphere, 307, 135577.
  • Andrade, M., Soares, A. M., Solé, M., Pereira, E., and Freitas, R. (2023). Threats of Pollutants Derived from Electronic Waste to Marine Bivalves: The Case of the Rare‐Earth Element Yttrium. Environmental Toxicology and Chemistry, 42(1), 166-177.
  • Bainy, A.C.D., Arisi, A.C.M., Azzalis, L.A., Simizu, K., Barros, S.B.M., Videla, L.A. and Junqueira, V.B.C. (1993). Differential effects of short-term lindane administration on parameters related to oxidative stress in rat liver and erythrocytes. Journal of Biochemical Toxicology, 8(4),187-194.
  • Ballesteros, M.L., Wunderlin, D.A. and Bistoni, M.A. (2009). Oxidative stress responses in different organs of Jenynsia multidentata exposed to endosulfan. Ecotoxicology and Environmental Safety, 72(1),199-205.
  • Barbosa, H., Soares, A. M., Pereira, E., and Freitas, R. (2023). Lithium: A review on concentrations and impacts in marine and coastal systems. Science of The Total Environment, 857, 159374.
  • Charalampides, G., Vatalis, K. I., Apostoplos, B., and Ploutarch-Nikolas, B. (2015). Rare earth elements: industrial applications and economic dependency of Europe. Procedia Economics and Finance, 24, 126-135.
  • Demidchik, V. (2015). Mechanisms of oxidative stress in plants: From classical chemistry to cell biology. Environmental and Experimental Botany, 109: 212- 228.
  • Dimitrova, M.S., Tishinova, V. and Velcheva, V. (1994). Combined effect of zinc and lead on the hepatic superoxide dismutase-catalase system in carp (Cyprinus carpio). Comp Biochem Physiol C: Pharmacol Toxicol Endocrinol, 108(1), 43-46.
  • Durmaz, H., Sevgiler. Y. and Üner, N. (2006). Tissuespecific antioxidative and neurotoxic responses to diazinon in Oreochromis niloticus. Pesticide Biochemistry and Physiology, 84(3), 215-226
  • Freitas, R., Cardoso, C. E., Costa, S., Morais, T., Moleiro, P., Lima, A. F., ... and Pereira, E. (2020). New insights on the impacts of e-waste towards marine bivalves: The case of the rare earth element Dysprosium. Environmental Pollution, 260, 113859.
  • Figueiredo, C., Grilo, TF, Lopes, C., Brito, P., Diniz, M., Caetano, M., ... and Raimundo, J. (2018). Cam yılan balıklarında (Anguilla anguilla) lantan maruziyeti altında birikim, eliminasyon ve nöro-oksidatif hasar. Chemosfer , 206 , 414-423.
  • Galdiero, E., Carotenuto, R., Siciliano, A., Libralato, G., Race, M., Lofrano, G. and Guida, M. (2019). Cerium and erbium effects on Daphnia magna generations: A multiple endpoints approach. Environmental Pollution, 254, 112985.
  • Gobi, N., Vaseeharan, B., Rekha, R., Vijayakumar, S., and Faggio, C. (2018). Bioaccumulation, cytotoxicity and oxidative stress of the acute exposure selenium in Oreochromis mossambicus. Ecotoxicology and environmental safety, 162, 147-159.
  • Hanana, H., Turcotte, P., André, C., Gagnon, C., and Gagné, F. (2017). Comparative study of the effects of gadolinium chloride and gadolinium–based magnetic resonance imaging contrast agent on freshwater mussel, Dreissena polymorpha. Chemosphere, 181, 197-207.
  • Hanana, H., Turcotte, P., Dubé, M., Gagnon, C., and Gagné, F. (2018). Response of the freshwater mussel, Dreissena polymorpha to sub-lethal concentrations of samarium and yttrium after chronic exposure. Ecotoxicology and environmental safety, 165, 662-670.
  • Hanana, H., Taranu, Z. E., Turcotte, P., Gagnon, C., Kowalczyk, J., and Gagné, F. (2021a). Sublethal effects of terbium and praseodymium in juvenile rainbow trout. Science of The Total Environment, 777, 146042.
  • Hanana, H., Taranu, ZE, Turcotte, P., Gagnon, C., Kowalczyk, J., and Gagné, F. (2021b). Nadir toprak elementleri disprosyum ve lutesyuma maruz kalan gökkuşağı alabalığında genel stres, detoksifikasyon yolları ve genotoksisitenin değerlendirilmesi. Ekotoksikoloji ve Çevre Güvenliği, 208, 111588.
  • Hu, Z., Richter, H., Sparovek, G., and Schnug, E. (2004). Physiological and biochemical effects of rare earth elements on plants and their agricultural significance: a review. Journal of plant nutrition, 27(1), 183-220.
  • Lavado, R., Ureña, R., Martin-Skilton, R., Torreblanca, A., Del Ramo, J., Raldua, D., and Porte, C. (2006). The combined use of chemical and biochemical markers to assess water quality along the Ebro River. Environmental Pollution, 139(2), 330-339.
  • Lebrun, J. D., Geffard, O., Urien, N., François, A., Uher, E., and Fechner, L. C. (2015). Seasonal variability and inter-species comparison of metal bioaccumulation in caged gammarids under urban diffuse contamination gradient: implications for biomonitoring investigations. Science of the Total Environment, 511, 501-508.
  • Lompré, J. S., Moleiro, P., De Marchi, L., Soares, A. M., Pretti, C., Chielini, F., and Freitas, R. (2021). Bioaccumulation and ecotoxicological responses of clams exposed to terbium and carbon nanotubes: Comparison between native (Ruditapes decussatus) and invasive (Ruditapes philippinarum) species. Science of The Total Environment, 784, 146914.
  • Migaszewski, Z. M., and Gałuszka, A. (2015). The characteristics, occurrence, and geochemical behavior of rare earth elements in the environment: a review. Critical reviews in environmental science and technology, 45(5), 429-471.
  • Pereira, L.V., Shimizu, M.H., Rodrigues, L.P., Leite, C.C., Andrade, L. and Seguro, A.C. (2012). Nacetylcysteine protects rats with chronic renal failure from gadolinium-chelate nephrotoxicity. Plos One, 7(7), e39528.
  • Pinto, J., Costa, M., Leite, C., Borges, C., Coppola, F., Henriques, B., Monteiro, R., Russo, T., Cosmo, A., Soares, MVN., Polese, G., Pereira, E., Freitas, R., 2019. Ecotoxicological effects of lanthanum in Mytilus galloprovincialis: Biochemical and histopathological impacts, Aquatic Toxicology, 211, 181-192.
  • Ronci, L., Meccoli, L., Iannilli, V., Menegoni, P., De Matthaeis, E., and Setini, A. (2016). Comparison between active and passive biomonitoring strategies for the assessment of genotoxicity and metal bioaccumulation in Echinogammarus veneris (Crustacea: Amphipoda). Italian Journal of Zoology, 83(2), 162-172.
  • Serdar, O., Yıldırım, N., Tatar, Ş., and Yildirim, N. C. (2021). Gadolinyumun Tatlı Su Omurgasızı Dreissena polymorpha Üzerindeki Biyokimyasal Etkileri. International Journal of Pure and Applied Sciences, 7(2), 229-236.
  • Sun, D., He, N., Chen, Q., and Duan, S. (2019). Effects of lanthanum on the photosystem II energy fluxes and antioxidant system of Chlorella Vulgaris and Phaeodactylum Tricornutum. International Journal of Environmental Research and Public Health, 16(12), 2242.
  • Sobjak, T.M., Romão, S., do Nascimento, C.Z., dos Santos, A.F.P., Vogel, L. and Guimarães, A.T.B. (2017). Assessment of the oxidative and neurotoxic effects of glyphosate pesticide on the larvae of Rhamdia quelen fish. Chemospher, 182, 267-275.
  • Tyler, G. (2004). Rare earth elements in soil and plant systems-A review. Plant and soil, 267, 191-206.
  • Tseng, M.T., Lu, X., Duan, X., Hardas, S.S., Sultana, R., Wu, P., Unrine, J.M., Graham, U., Butterfield, D.A., Grulke, E.A. and Yokel, R.A. (2012). Alterationofhepaticstructure and oxidative stress induced by intravenous nanoceria. Toxicology and Applied Pharmacology, 260, 173–182.
  • URL-1: https://evrimagaci.org/terbiyum-10139 (14.04.2023).
  • Van der Oost, R., Beyer, J., and Vermeulen, N. P. (2003). Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environmental toxicology and pharmacology, 13(2), 57-149.
  • Wang, L., Wang, W., Zhou, Q.. and Huang, X. (2014). Combined effects of lanthanum (III) chloride and acid rain on photosynthetic parameters in rice. Chemosphere, 112, 355–361.
  • Wang, L., Zhang, X., Wu, L., Liu, Q., Zhang, D., and Yin, J. (2018). Expression of selenoprotein genes in muscle is crucial for the growth of rainbow trout (Oncorhynchus mykiss) fed diets supplemented with selenium yeast. Aquaculture, 492, 82-90.
  • Zhao, H., Hong, J., Yu, X., Zhao, X., Sheng, L., Ze, Y., Sang, X., Gui, S., Sun, Q., Wang, L.. and Hong, F. (2013). Oxidative stress in the kidney injury of mice following exposure to lanthanides trichloride. Chemosphere, 93, 875–884.
There are 37 citations in total.

Details

Primary Language Turkish
Subjects Structural Biology
Journal Section Research Articles
Authors

Ayşe Nur Aydın 0000-0002-5657-8958

Osman Serdar 0000-0003-1744-8883

Early Pub Date October 19, 2023
Publication Date March 1, 2024
Published in Issue Year 2024 Volume: 20 Issue: 1

Cite

APA Aydın, A. N., & Serdar, O. (2024). Terbiyumun Pontastacus leptodactylus’ta ki Oksidatif Stres ve Antioksidan Yanıtlarının Belirlenmesi. Acta Aquatica Turcica, 20(1), 23-32. https://doi.org/10.22392/actaquatr.1294250