Determination of Terbium's Oxidative Stress and Antioxidant Responses in Pontastacus leptodactylus

Year 2024, Volume: 20 Issue: 1, 23 - 32, 01.03.2024

Ayşe Nur Aydın , Osman Serdar

https://doi.org/10.22392/actaquatr.1294250

Abstract

Terbium (Tb) is a rare earth element (REE) that can become toxic to aquatic organisms when present in high concentrations in the aquatic environment. There are still not enough studies on the oxidative stress consequences of Tb toxicity in the freshwater ecosystem. In this study, the freshwater creature Pontastacus leptodactylus was used to evaluate the oxidative stress consequences of Tb. The oxidative stress and antioxidant changes caused by Tb at 1, 5 and 25 mg/L concentrations at 24 and 96 hours in P. leptodactylus, were affected by the enzyme activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and thiobarbituric acid (TBARS) and reduced levels of glutathione (GSH) were determined. Results were determined using ELISA kits. SPSS 24.0 package program one-way ANOVA (Duncan 0.05) test was used in the statistical evaluation of biomarker analyses. While no significant change was observed in SOD activity (p>0.05) compared to the control group, significant decreases were observed in CAT and GPx activities (p<0.05); Significant (p<0.05) increases were observed in GSH and TBARS levels. Considering the study data, it is concluded that the TB xenobiotic that penetrates the body of living organisms causes oxidative stress in the organism.

Keywords

terbium, pontastacus leptodactylus, oxidative stres, biomarkers, antoxidant

Terbiyumun Pontastacus leptodactylus’ta ki Oksidatif Stres ve Antioksidan Yanıtlarının Belirlenmesi

Abstract

Terbiyum (Tb), su ortamında yüksek konsantrasyonlarda bulunduğunda sucul organizmalar için toksik hale gelebilen nadir toprak elementi (NTE)’dir. Tatlı su ekosistemindeki Tb toksisitesinin meydana getirdiği oksidatif stres sonuçlarıyla ilgili hala yeterince çalışmalar mevcut değildir. Bu çalışma ile Tb'nin oksidatif stres sonuçlarını değerlendirmek için tatlı su canlısı olan Pontastacus leptodactylus kullanılmıştır. Bu organizmada, 1, 5 ve 25 mg/L konsantrasyonlardaki Tb'nin 24 ve 96. saatte meydana getirdiği oksidatif stres ve antioksidan değişimleri süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px), katalaz (CAT) enzim aktiviteleri ile Tiyobarbitürik asit (TBARS) ve redükte glutatyon (GSH) seviyeleri belirlenmiştir. Sonuçlar ELISA kitleri kullanılarak tespit edilmiştir. Biyobelirteç analizlerin istatatistiksel değerlendirilmesinde SPSS 24.0 paket program tek-yönlü ANOVA (Duncan 0,05) testi kullanılmıştır. Kontrol grubu ile karşılaştırıldığında SOD aktivitesinde önemli bir değişim gözlemlenmezken (p>0,05) CAT ve GPx aktivitelerinde anlamlı azalmalar (p<0,05) gözlemlenmiştir; GSH ve TBARS seviyelerinde ise anlamlı (p<0,05) artışlar gözlemlenmiştir. Çalışma verileri dikkate alındığında canlı organizma vücuduna nüfuz eden Tb ksenobiyotiğinin organizma da oksidatif strese neden olduğu düşünülmektedir.

Keywords

terbiyum, pontastacus leptodactylus, oksidatif stres, antioksidan, biyobelirteç

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