Determination of In Vitro Antibiotic Susceptibility of Campylobacter species Isolated from Stool Samples and Detection of Macrolide Resistance by Genotypic Methods

Year 2024, Volume: 38 Issue: 1, 12 - 17, 30.04.2024

Gülşen Hazırolan Gizem Ekiz Hazal Gür Şeyma Demirsoy Yakut Akyön Yılmaz

https://doi.org/10.54962/ankemderg.1432254

Abstract

Most gastroenteritis caused by Campylobacter are self-limiting. However, antimicrobial treatment (e.g., macrolides, fluoroquinolones) is necessary in severe or prolonged cases. The objective of this study was to identify Campylobacter species isolated from stool samples, to determine in vitro antibiotic susceptibility and detect macrolide resistance by genotypic methods. We analyzed a total of 65 strains that were isolated from stool samples between August 2014 and August 2019. Antibiotic susceptibilities of the isolates were detected by disk diffusion method and the results were evaluated according to the European Committee of Antimicrobial Susceptibility Testing (EUCAST)guidelines. Macrolide resistance was investigated by mismatch amplification mutation assay- polymerase chain reaction (MAMA-PCR). Of the isolates, 60 (92.3%) were identified as Campylobacter jejuni, five (7.7%) were Campylobacter coli. The rates of resistance of the isolates were 76.92% for ciprofloxacin and 16.92 % for erythromycin. In 11 macrolide resistant isolates, 23S rRNA, ERY2074 and/or ERY2075 mutations were detected. Isolated ERY2025 and ERY2024 mutations were observed in five and three isolates, respectively. The high presence of resistance to macrolides and quinolones, which are used to treat campylobacteriosis in humans, highlights the need for proper antimicrobial surveillance and control measures.

Keywords

Campylobacter species, erythromycin, ciprofloxacin, in vitro resistance, MAMA-PCR

Dışkı örneklerinden izole edilen Campylobacter türlerinin in vitro antibiyotik duyarlılıklarının belirlenmesi ve makrolid direncinin genotipik yöntemlerle saptanması.

Abstract

Campylobacter enfeksiyonu genellikle kendi kendini sınırlar, ancak ciddi veya uzun süreli vakalarda antimikrobiyal tedavi (örn. makrolidler, florokinolonlar) gereklidir. Bu çalışmanın amacı dışkı örneklerinden izole edilen Campylobacter türlerinde, in vitro antibiyotik duyarlılığı saptamak ve makrolid direncini genotipik yöntemlerle tespit etmektir. Ağustos 2014 ile
Ağustos 2019 arasında dışkı örneklerinden elde edilen toplam 65 Campylobacter izolatı analiz edildi. İzolatların antibiyotik duyarlılıkları gradiyent test yöntemiyle tespit edildi. Sonuçlar Avrupa Antimikrobiyal Duyarlılık Testi Komitesi (European Committee of Antimicrobial Susceptibility Testing - EUCAST) kılavuzuna göre değerlendirildi. Makrolid direnci uyumsuzluk amplifikasyon mutasyon testi (mismatch amplification mutation assay) polimeraz zincir reaksiyonu
(MAMA-PZR)ile araştırıldı. Altmış (%92.3) izolat C.jejuni ve beş (%7.7) izolat C. coli olarak tanımlandı. İzolatların direnç oranları siprofloksasine %76.92, eritromisine ise %16.92 olarak tespit edildi. Makrolid dirençli 11 izolatta, 23S rRNA, ERY2074 ve/veya ERY2075 mutasyonu tespit edildi. Sadece 23S rRNA, ERY2025 ve ERY2024 mutasyonu sırasıyla beş ve üç izolatta gözlendi. İnsanlarda kampilobakteriyozu tedavi etmek için kullanılan makrolidler ve kinolonlara karşı yüksek direnç oranları, uygun antimikrobiyal gözetim ve kontrol önlemlerine olan ihtiyacı vurgulamaktadır.

Keywords

Campylobacter türleri, eritromisin, siprofloksasin, in vitro direnç, MAMA-PZR

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