Investigation of the Phenolic Contents and Antioxidant Activities of Some Natural Edible Mushroom Species

Year 2024, Volume: 26 Issue: 1, 7 - 12, 29.02.2024

Mertcan Karadeniz Temel Kan Bakır Sabri Ünal

https://doi.org/10.24011/barofd.1386438

Abstract

In recent years, edible mushroom species have become a part of the daily human diet due to their high protein content. These mushrooms have also gained popularity in alternative medicine practices due to their chemical composition and antioxidant properties. This study aimed to determine the biologically important antioxidant activities and total phenolic content of four different mushroom species, each with its unique appearance and habitat: Amanita caesarea (Aca), Clitocybe geotropa (Cge), Cordyceps militaris (Cmi) and Lentinula edodes (Led). The antioxidant activities of the mushroom species were determined using the DPPH radical scavenging method, and the percentage inhibition and IC50 values were reported. The analysis of inhibition values at various concentrations revealed that both Cmi and Aca mushrooms demonstrated higher antioxidant activity when compared to Led and Cge mushrooms across all tested concentrations. Moreover, the phenolic content of the methanolic extracts, quantified in gallic acid equivalents (GAE), were determined to be 37.04±0.35, 52.04±0.41, 19.33±0.11, and 21.63±0.15 mg GAE/g for Cmi, Aca, Led, and Cge, respectively. In conclusion, a direct correlation was noted between the overall phenolic content and the antioxidant activity of the various mushroom species.

Keywords

DPPH radical scavenging method, Amanita caesarea, Clitocybe geotropa, Cordyceps militaris, Lentinula edodes.

Doğal Tüketilebilir Bazı Mantar Türlerinin Fenolik İçeriklerinin ve Antioksidan Aktivitelerinin Araştırılması

Abstract

Son yıllarda yenilebilir mantar türleri, yüksek protein içeriğinden dolayı günlük insan beslenmesinin bir parçası haline gelmiştir. Bu mantarlar kimyasal bileşimleri ve antioksidan özellikleri nedeniyle alternatif tıp uygulamalarında da popülerlik kazanmıştır. Bu çalışma, her biri kendine özgü görünüm ve yaşam ortamına sahip dört farklı mantar türünün biyolojik olarak önemli antioksidan aktivitelerini ve toplam fenolik içeriğini belirlemeyi amaçlamıştır: Amanita caesarea (Aca), Clitocybe geotropa (Cge), Cordyceps militaris (Cmi) ve Lentinula edodes (Led). Mantar türlerinin antioksidan aktiviteleri DPPH radikal süpürme yöntemi kullanılarak belirlenmiş ve yüzde inhibisyon ve IC50 değerleri rapor edilmiştir. Farklı konsantrasyonlarda elde edilen inhibisyon değerlerine göre Cmi ve Aca mantarlarının her konsantrasyonda Led ve Cge mantarlarına göre daha yüksek antioksidan aktivite sergilediği tespit edilmiştir. Ayrıca, metanolik özütlerin fenolik içeriği galik asit eşdeğerleri (GAE) cinsinden belirlenmiş olup, Cmi için 37.04±0.35, Aca için 52.04±0.41, Led için 19.33±0.11 ve Cge için 21.63±0.15 mg GAE/g olarak tespit edilmiştir. Sonuç olarak mantar türlerinin toplam fenolik içeriği ile antioksidan aktivitesi arasında doğrusal bir ilişki gözlendi.

Keywords

DPPH radikal sönümleme metodu, Amanita caesarea, Clitocybe geotropa, Cordyceps militaris, Lentinula edodes.

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