Research Article
BibTex RIS Cite

Investigation of the antioxidant and antibacterial effects of fermented Cornus mas and Rubus sanctus fruits

Year 2023, Volume: 4 Issue: 2, 62 - 67, 31.12.2023
https://doi.org/10.51539/biotech.1374327

Abstract

In this study antioxidant and antibacterial activities of fermented Cornus mas and Rubus sanctus berries collected from province of Bartın in the Western Black Sea region of Türkiye were analyzed. Prior to fermentation with S. cerevisiae, the fruits were tested for 58 pesticides such as Dicloran and Quintozene and none of the pesticides were detected. The presence of ascorbic acid in the fruits, which is a nutrient needed by the body, was also detected by FTIR. Then the pesticide free berries were crushed, and the samples were fermented separately. Testing after the fermentation process revealed the samples contained ethyl alcohol. Antioxidant activities of fermented samples were analyzed using CUPRAC, DPPH and Folin Ciocalteu methods. The results suggest high antioxidant contents of the fermented samples. Evaluation of antimicrobial activity was done through disk diffusion method using P.aeruginosa and S.aureus suggesting that these samples do not suppress these bacteria for the studied concentrations. Furthermore, the growth of C. albicans was examined immediately, demonstrating that the fermented samples do not show antifungal effects. The reason for these shortcomings could be inadequate concentration levels. The antioxidant content of these fermented fruits is intended to contribute to human health.

References

  • Apak R, Güçlü K, Demirata B, Özyürek M, Çelik SE, Bektaşoğlu B, Berker KI, Özyurt D. (2007) Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules 12(7). doi:10.3390/12071496
  • Aurori M, Niculae M, Hanganu D, Pall E, Cenariu M, Vodnar DC, Bunea A, Fiţ N, Andrei S (2023) Phytochemical Profile, Antioxidant, Antimicrobial and Cytoprotective Effects of Cornelian Cherry (Cornus mas L.) Fruit Extracts. Pharmaceuticals (Basel) 16(3):420
  • Bayer A, Kirby W, Sherris J, Turck M (1966) Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol 45(4):493-496.
  • Beyer RE (1994) The role of ascorbate in antioxidant protection of biomembranes: interaction with vitamin E and coenzyme Q. J Bioenerg Biomembr. 26(4):349-58
  • Collins C (2004) Collins and Lyne’s Microbiological Methods. 8 ed. USA: CRC Press, 175-190
  • Damalas CA, Eleftherohorinos IG (2011) Pesticide exposure, safety issues, and risk assessment indicators. Int J Environ Res Public Health. 8(5):1402-1419
  • Gulcin İ (2020) Antioxidants and antioxidant methods: an updated overview. Arch Toxicol 94(3). doi:10.1007/s00204-020-02689-3
  • Lobo V, Patil A, Phatak A, Chandra N (2010) Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 4(8) doi:10.4103/0973-7847.70902
  • Lussignoli S, Fraccarolli M, Andriolli G, Brocco G, Bellavite P (1999) A microplatebased colorimetric assay of the total peroxyl radical trapping capability of human plasma. Analytic Biochemistry 269:38-44.
  • Olas B. (2018) Berry Phenolic Antioxidants - Implications for Human Health? Front Pharmacol 9:78
  • Ponder A, Hallmann E (2020) The nutritional value and vitamin C content of different raspberry cultivars from organic and conventional production, Journal of Food Composition and Analysis, 87,103429
  • Sanchez-Moreno C, Larrauri JA, Saura-Calixto F (1999) Free radical scavenging capacity of selected red, rose and white wines. J Sci Food Agri 79:1301–1304.
  • Song NE, Jeong DY, Baik SH (2018) Application of indigenous Saccharomyces cerevisiae to improve the black raspberry (Rubus coreanus Miquel) vinegar fermentation process and its microbiological and physicochemical analysis. Food Sci Biotechnol 28(2):481-489.
  • Soong YY, Barlow PJ, Tamez Uddin M, et al. (2019) Bioactive Molecules in Food. Vol 65.
  • Şanlier N, Gökcen BB, Sezgin AC (2019) Health benefits of fermented foods. Crit Rev Food Sci Nutr 59(3). doi:10.1080/10408398.2017.1383355
  • Tiptiri-Kourpeti A, Fitsiou E, Spyridopoulou K, Vasileiadis S, Iliopoulos C, Galanis A, Vekiari S, Pappa A, Chlichlia K (2019) Evaluation of Antioxidant and Antiproliferative Properties of Cornus mas L. Fruit Juice. Antioxidants (Basel). 8(9):377
  • Zagórska-Dziok M, Ziemlewska A, Mokrzyńska A, Nizioł-Łukaszewska Z, Sowa I, Szczepanek D, Wójciak M (2023) Comparative Study of Cytotoxicity and Antioxidant, Anti-Aging and Antibacterial Properties of Unfermented and Fermented Extract of Cornus mas L. Int J Mol Sci 24(17):13232
  • Zengin G, Ferrante C, Senkardes I, Gevrenova R, Zheleva-Dimitrova D, Menghini L, Orlando G, Recinella L, Chiavaroli A, Leone S, Brunetti L, Nancy Picot-Allain CM, Rengasamy KRR, Mahomoodally MF (2019)
  • Multidirectional biological investigation and phytochemical profile of Rubus sanctus and Rubus ibericus. Food Chem Toxicol 127:237-250
Year 2023, Volume: 4 Issue: 2, 62 - 67, 31.12.2023
https://doi.org/10.51539/biotech.1374327

Abstract

References

  • Apak R, Güçlü K, Demirata B, Özyürek M, Çelik SE, Bektaşoğlu B, Berker KI, Özyurt D. (2007) Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules 12(7). doi:10.3390/12071496
  • Aurori M, Niculae M, Hanganu D, Pall E, Cenariu M, Vodnar DC, Bunea A, Fiţ N, Andrei S (2023) Phytochemical Profile, Antioxidant, Antimicrobial and Cytoprotective Effects of Cornelian Cherry (Cornus mas L.) Fruit Extracts. Pharmaceuticals (Basel) 16(3):420
  • Bayer A, Kirby W, Sherris J, Turck M (1966) Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol 45(4):493-496.
  • Beyer RE (1994) The role of ascorbate in antioxidant protection of biomembranes: interaction with vitamin E and coenzyme Q. J Bioenerg Biomembr. 26(4):349-58
  • Collins C (2004) Collins and Lyne’s Microbiological Methods. 8 ed. USA: CRC Press, 175-190
  • Damalas CA, Eleftherohorinos IG (2011) Pesticide exposure, safety issues, and risk assessment indicators. Int J Environ Res Public Health. 8(5):1402-1419
  • Gulcin İ (2020) Antioxidants and antioxidant methods: an updated overview. Arch Toxicol 94(3). doi:10.1007/s00204-020-02689-3
  • Lobo V, Patil A, Phatak A, Chandra N (2010) Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 4(8) doi:10.4103/0973-7847.70902
  • Lussignoli S, Fraccarolli M, Andriolli G, Brocco G, Bellavite P (1999) A microplatebased colorimetric assay of the total peroxyl radical trapping capability of human plasma. Analytic Biochemistry 269:38-44.
  • Olas B. (2018) Berry Phenolic Antioxidants - Implications for Human Health? Front Pharmacol 9:78
  • Ponder A, Hallmann E (2020) The nutritional value and vitamin C content of different raspberry cultivars from organic and conventional production, Journal of Food Composition and Analysis, 87,103429
  • Sanchez-Moreno C, Larrauri JA, Saura-Calixto F (1999) Free radical scavenging capacity of selected red, rose and white wines. J Sci Food Agri 79:1301–1304.
  • Song NE, Jeong DY, Baik SH (2018) Application of indigenous Saccharomyces cerevisiae to improve the black raspberry (Rubus coreanus Miquel) vinegar fermentation process and its microbiological and physicochemical analysis. Food Sci Biotechnol 28(2):481-489.
  • Soong YY, Barlow PJ, Tamez Uddin M, et al. (2019) Bioactive Molecules in Food. Vol 65.
  • Şanlier N, Gökcen BB, Sezgin AC (2019) Health benefits of fermented foods. Crit Rev Food Sci Nutr 59(3). doi:10.1080/10408398.2017.1383355
  • Tiptiri-Kourpeti A, Fitsiou E, Spyridopoulou K, Vasileiadis S, Iliopoulos C, Galanis A, Vekiari S, Pappa A, Chlichlia K (2019) Evaluation of Antioxidant and Antiproliferative Properties of Cornus mas L. Fruit Juice. Antioxidants (Basel). 8(9):377
  • Zagórska-Dziok M, Ziemlewska A, Mokrzyńska A, Nizioł-Łukaszewska Z, Sowa I, Szczepanek D, Wójciak M (2023) Comparative Study of Cytotoxicity and Antioxidant, Anti-Aging and Antibacterial Properties of Unfermented and Fermented Extract of Cornus mas L. Int J Mol Sci 24(17):13232
  • Zengin G, Ferrante C, Senkardes I, Gevrenova R, Zheleva-Dimitrova D, Menghini L, Orlando G, Recinella L, Chiavaroli A, Leone S, Brunetti L, Nancy Picot-Allain CM, Rengasamy KRR, Mahomoodally MF (2019)
  • Multidirectional biological investigation and phytochemical profile of Rubus sanctus and Rubus ibericus. Food Chem Toxicol 127:237-250
There are 19 citations in total.

Details

Primary Language English
Subjects Plant Biotechnology
Journal Section Research Articles
Authors

Derya Ünal This is me

Tuba Sevimoğlu 0000-0003-4563-3154

Early Pub Date December 25, 2023
Publication Date December 31, 2023
Submission Date October 11, 2023
Acceptance Date December 10, 2023
Published in Issue Year 2023 Volume: 4 Issue: 2

Cite

APA Ünal, D., & Sevimoğlu, T. (2023). Investigation of the antioxidant and antibacterial effects of fermented Cornus mas and Rubus sanctus fruits. Bulletin of Biotechnology, 4(2), 62-67. https://doi.org/10.51539/biotech.1374327
AMA Ünal D, Sevimoğlu T. Investigation of the antioxidant and antibacterial effects of fermented Cornus mas and Rubus sanctus fruits. Bull. Biotechnol. December 2023;4(2):62-67. doi:10.51539/biotech.1374327
Chicago Ünal, Derya, and Tuba Sevimoğlu. “Investigation of the Antioxidant and Antibacterial Effects of Fermented Cornus Mas and Rubus Sanctus Fruits”. Bulletin of Biotechnology 4, no. 2 (December 2023): 62-67. https://doi.org/10.51539/biotech.1374327.
EndNote Ünal D, Sevimoğlu T (December 1, 2023) Investigation of the antioxidant and antibacterial effects of fermented Cornus mas and Rubus sanctus fruits. Bulletin of Biotechnology 4 2 62–67.
IEEE D. Ünal and T. Sevimoğlu, “Investigation of the antioxidant and antibacterial effects of fermented Cornus mas and Rubus sanctus fruits”, Bull. Biotechnol., vol. 4, no. 2, pp. 62–67, 2023, doi: 10.51539/biotech.1374327.
ISNAD Ünal, Derya - Sevimoğlu, Tuba. “Investigation of the Antioxidant and Antibacterial Effects of Fermented Cornus Mas and Rubus Sanctus Fruits”. Bulletin of Biotechnology 4/2 (December 2023), 62-67. https://doi.org/10.51539/biotech.1374327.
JAMA Ünal D, Sevimoğlu T. Investigation of the antioxidant and antibacterial effects of fermented Cornus mas and Rubus sanctus fruits. Bull. Biotechnol. 2023;4:62–67.
MLA Ünal, Derya and Tuba Sevimoğlu. “Investigation of the Antioxidant and Antibacterial Effects of Fermented Cornus Mas and Rubus Sanctus Fruits”. Bulletin of Biotechnology, vol. 4, no. 2, 2023, pp. 62-67, doi:10.51539/biotech.1374327.
Vancouver Ünal D, Sevimoğlu T. Investigation of the antioxidant and antibacterial effects of fermented Cornus mas and Rubus sanctus fruits. Bull. Biotechnol. 2023;4(2):62-7.