HOST AND PLANT TAXONOMY AS INFLUENTIAL FACTORS IN CONTROLING IN VITRO SPECIFIC CELL-MEDIATED RESPONSE IN HERBIVORES

Year 2023, Volume: 6 Issue: 2, 78 - 83, 25.12.2023

Vasiu Aurel Carmen Sandru Emoke Pall Emilia Ungureanu Florina Marian Silvana Popescu Alexandra Oprescu Ciprian Ilasca

https://doi.org/10.38093/cupmap.1337829

Abstract

Objective: This study monitored several alcoholic vegetal extracts from the adjuvant and immune cell stimulating
perspective in farmed herbivores, differing by their digestive physiology.
Material and Methods: Blood from randomly selected farmed ruminants (Romanian Spotted dairy cows, n=28
and Angora goats, n=19) and Romanian draft horses (n=27) was used to measure the in vitro effects on specific
cell-mediated reactivity of alcoholic extracts of Calendula officinalis, Echinacea angustifolia and E. purpurea, by the
in vitro whole blood blast transformation test, in 96 well plates, after 60 to 72h of incubation. Cell growth was
quantified by an orto-toluidine technique. Student’s t- test was used to evaluate the statistical significance of the
differences.
Results&Discussion: The in vitro growth indices were lowest in goats (C. officinalis - 58.52±10.02%,
E.angustifolia - 50.06±11.67%, E.purpurea -50.79±10.98%) and higher in bovine (C. officinalis – 69.9±2.65%,
E.angustifolia - 74.9±10.1%, p<0.05), and increased towards E. angustifolia versus C. officinalis.
In vitro responses to C. officinalis, E. angustifolia and E.purpurea were the most pronounced in horses and similar
for all of these extracts.
Conclusion: All the extracts showed inhibiting effects in bovine and goats, but not in horses, supporting host-
based differences. The biological activity of the tested extracts was plant species dependent.

Keywords

ruminants, horses, plant extracts, blast transformation

Thanks

The research was supported by current funds of the Discipline of Infectious diseases. The authors wish to thank for laboratory assistance to biotechnologist Eng. Elena Marian and for technical support to senior technician Traian Jucan.

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