Effect of Solvent Polarity on Extraction Yield of Total Flavonoids with Special Emphasis to Glabridin from Glycyrrhiza glabra Roots

Year 2022, Volume: 1 Issue: 47, 1 - 12, 01.03.2022

Sadanand Yewale Zeba Farash Shrikant Kulkarnı Shital Palghadmal Neelam Athawale Laxman Sawant Shrinivas Bhope Sriram Padmanabhan

https://doi.org/10.55262/fabadeczacilik.1078751

Cited By: 2

Abstract

Different organic solvents (ethanol, dichloromethane, ethyl acetate and acetone) were studied for their effects on the extraction efficiency of glabridin and total flavonoids (TF) from Glycyrrhiza glabra roots. The extract yield of Glycyrrhiza glabra roots was in the range of 3% to 6% following the extraction efficiency in the order ethanol>acetone>ethyl acetate>dichloromethane. A higher extraction yield of TF and glabridin was obtained with dichloromethane, followed by ethyl acetate, acetone and ethanol, indicating that the non-polar solvents help in optimal extraction of TF and glabridin. We also demonstrate for the first time, that the extraction efficiency of the flavonoids is not significantly affected by the use of the recovered solvents except in case of ethanol which reflects that the moisture absorbing capacity of the solvent dictates the extraction efficiency of such compounds. The glycyrrhizin content in all the extract types was rather low (0.1 % to 1%) except for extract prepared with water, where the glycyrrhizin content was ~10% as expected since glycyrrhizin is a polar compound. Interestingly, we observed that ethyl acetate selectively isolated only glabridin with no traces of glycyrrhizin, which is a finding reported for the first time.

Keywords

Licorice, Glabridin, Flavonoids, Glycyrrhizin, Extraction, Solvent polarity

Çözücü Polaritesinin Özellikle Glabridin Olmak Üzere Glycyrrhiza glabra Köklerindeki Toplam Flavonoitlerin Ekstraksiyonunun Verimine Etkisi

Abstract

Farklı organik çözücülerin (etanol, diklorometan, etil asetat ve aseton) Glycyrrhiza glabra köklerinden glabridin ve toplam flavonoit (TF) ekstraksiyon verimliliğine etkileri çalışılmıştır.
Glycyrrhiza glabra köklerinden ekstre verimi % 3 ile % 6 aralığında, ekstraksiyon etkinliği etanol> aseton> etil asetat> diklorometan sırası ile bulunmuştur. Diklorometan, ardından etil asetat, aseton ve etanol ile daha yüksek bir TF ve glabridin ektraksiyon verimi elde edilmiştir, bu da TF ve glabridinin en uygun şekilde ekstraksiyonunda polar olmayan çözücülerin etkili olduğunu göstermektedir. Ayrıca flavonoitlerin ektraksiyon verimliliğinin, çözücünün nem absorplama kapasitesinin bu tür bileşiklerin ekstre edilmesinde rol oynadığını yansıtan etanol haricinde, geri kazanılan çözücülerin kullanımından büyük ölçüde etkilenmediği de ilk kez gösterilmiştir. Su ile hazırlanan ekstre haricinde tüm ekstre türlerinde glisirizin içeriği düşük olup % 0,1 ile % 1 aralığındadır, bileşik polar bir bileşik olduğu için su ekstresinde içerik beklendiği gibi ~% 10’dur. İlginç bir şekilde, etil asetatın glisirizin izleri olmadan sadece glabridini seçici olarak izole ettiği ilk kez bildirilen bir bulgu olarak gözlenmiştir.

Keywords

Meyankökü, Glabridin, Flavonoitler, Glisirizin, Ekstraksiyon, Çözücü polaritesi

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