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Chemical Composition and Phytochemical Screening of Juniperus Phoenicea L.: Evaluation of Antioxidant Activity, Minerals, and Bioactive Compounds

Year 2024, Volume: 11 Issue: 2, 709 - 722, 15.05.2024
https://doi.org/10.18596/jotcsa.1330273

Abstract

The purpose of the research was to determine Juniperus phoenicea L.'s elemental content, antioxidant activity, and phytochemical composition. Phytochemical screening was performed on four plant extracts (water, ethanol, chloroform, and ether). The aqueous and ethanol extracts were also analyzed for their total phenols, total flavonoids, and total antioxidant contents. The levels of macroelements (Na, Mg, Ca) and microelements (Fe, Cu, Zn) in the plant were determined by flame photometry and atomic absorption spectrometry. Moisture, ash, total protein, and total alkaloids were also determined. The results showed that the aqueous and ethanol extracts contained various phytochemicals, such as carbohydrates, proteins, phenols, tannins, flavonoids, alkaloids, coumarins, anthocyanins, saponins, and glycosides. The ethanol extract had higher concentrations of most phytochemicals than the aqueous extract, except for carbohydrates and proteins. The chloroform and ether extracts had lower concentrations of phytochemicals than the aqueous and ethanol extracts. The moisture, ash, total protein, and total alkaloid contents of the plant were 13%, 5.52%, 10.78%, and 1.84%, respectively. The total phenol contents, total flavonoid contents, and total antioxidant activity of the ethanol extract were 49.36±5.24 mg/g, 20.61±2.08 mg/g, and 34.82±2.44 mg/g, respectively. The corresponding values for the aqueous extract were 46.26±2.47 mg/g, 14.80±1.12 mg/g, and 37.32±3.29 mg/g, respectively. The order of abundance for macroelements was Ca (26860±950 mg/kg) > Na (1705.4±85 mg/kg) > Mg (944.4±38 mg/kg), whereas for microelements it was Fe (315.4±18 mg/kg) > Cu (55.52±3 mg/kg) > Zn (35.66±2 mg/kg). These results indicate that Juniperus phoenicea L. is a rich source of phytochemicals and elements that may have potential health benefits.

References

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Year 2024, Volume: 11 Issue: 2, 709 - 722, 15.05.2024
https://doi.org/10.18596/jotcsa.1330273

Abstract

References

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  • 26. Yaghi MM, Mohammed MY, Towier NA, Elsherif KM. Analysis of Potato Chips: Evaluation of Sodium, Potassium and Chloride Contents. Int J Multidiscip Sci Adv Technol. 2021;1(Special Issue):92-97. Available from: <URL>
  • 27. Elsherif KM, Abu Khater RA, Hegaig FA. Determination of major and minor elements in dairy products produced in Misurata city – Libya. Maghrebian J Pure Appl Sci. 2017;3(2):9-17.
  • 28. Di Marzo L, Pranata J, Barbano DM. Measurement of casein in milk by Kjeldahl and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J Dairy Sci. 2021;104(7):7448-7456. Available from: <URL>
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  • 30. Rumpf J, Burger R, Schulze M. Statistical evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins. Int J Biol Macromol. 2023;233:123470. Available from: <URL>
  • 31 Shraim AM, Ahmed TA, Rahman MM, Hijji YM. Determination of total flavonoid content by aluminum chloride assay: A critical evaluation. LWT - Food Sci Technol. 2021;150:111932. Available from: <URL>
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  • 34. Alkherraz A, Hashad O, Elsherif K. Heavy metals contents in some commercially available coffee, tea, and cocoa samples in Misurata City–Libya. Prog Chem Biochem Res. 2019;2(3):99-107.
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There are 62 citations in total.

Details

Primary Language English
Subjects Biochemistry and Cell Biology (Other)
Journal Section RESEARCH ARTICLES
Authors

Khaled Muftah Elsherif 0000-0002-3884-1804

Marwa A. Sulaiman This is me 0009-0001-0770-4583

Adel Mlitan This is me 0009-0001-5536-4798

Publication Date May 15, 2024
Submission Date July 23, 2023
Acceptance Date February 9, 2024
Published in Issue Year 2024 Volume: 11 Issue: 2

Cite

Vancouver Elsherif KM, Sulaiman MA, Mlitan A. Chemical Composition and Phytochemical Screening of Juniperus Phoenicea L.: Evaluation of Antioxidant Activity, Minerals, and Bioactive Compounds. JOTCSA. 2024;11(2):709-22.