Yozgat Bozok Üniversitesi, BAP Birimi
BAP projesi, 6601-FBE/19-256
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Differential display analyses of expressional modulations occurring in plant-microbe interactions provide valuable information about the physiological changes and underlying genetic factors and mechanisms involved in host resistance and susceptibility to disease. We carried out a differential display analysis study in the compatible interaction of Z. mays and P. sorghi and identified 98 Transcript Derived Fragments (TDFs) as expressional modulation showing tags. 72 TDFs were cloned and sequenced. Sequence database similarity searches revealed that there is at least one close matching GenBank record for each TDF. Compiled results showed that approximately half of the TDFs were derived from genes with known functions, about a half of which are known to display expressional modulations in plant-microbe interactions. CA, BiP2, An2, ARP1 and ASR3-like protein encoding sequence similar TDFs constituted the prominent examples of this group. A large proportion of TDFs were found to be similar to uncharacterized/hypothetical protein encoding GenBank records. RT-qPCR primers were designed for Bip2, CA, cALDH, ARP and Arpp3 genes to verify their observed expressional modulations, which were generally confirmed except for CA. Although a limited number of TDFs were characterized, overall, the results provide an overview to the expressional modulation showing genes in the compatible interaction of the studied pathosystem.
BAP projesi, 6601-FBE/19-256
Primary Language | Turkish |
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Subjects | Agricultural Engineering |
Journal Section | Makaleler |
Authors | |
Project Number | BAP projesi, 6601-FBE/19-256 |
Publication Date | August 1, 2020 |
Submission Date | January 20, 2020 |
Published in Issue | Year 2020 Volume: 33 Issue: 2 |
Mediterranean Agricultural Sciences is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.