Capnodis tenebrionis L.'den izole edilen bakterilerin izolasyonu, karakterizasyonu ve bazı böcek zararlılarına karşı biyolojik mücadele potansiyelleri

Year 2023, Volume: 14 Issue: 2, 85 - 104, 15.01.2024

Ebru Güney Aydın Kazım Sezen

https://doi.org/10.31019/tbmd.1311645

Abstract

Capnodis tenebrionis L. (Coleoptera: Buprestidae), Türkiye'nin de dahil olduğu birçok ülkede sert çekirdekli meyve bahçelerinin en yıkıcı zararlılarından biridir. Bu çalışmada C. tenebrionis'in larva ve erginlerinden 21 bakteri izolatı izole edilmiş, morfolojik, fizyolojik, biyokimyasal ve moleküler özellikleri belirlenmiştir. Buna göre C. tenebrionis'in kültürlenebilir bakteri florası; Bacillus cereus, B. mycoides, B. pumilus, Paenibacillus xylanilyticus, B. flexus, B. simplex, Raoultella terrigena, Enterobacter cloacae, Klebsiella oxytoca, B. safensis, B. amyloliquefaciens ve B. aryabhattai olarak saptanmıştır. Bu bakteri izolatlarının farklı zararlı böcek türlerinin larvalarına karşı biyolojik mücadele potansiyelleri; Karaağaç yaprak böceği (Pyrrhalta luteola M.), Petek güvesi (Galleria mellonella L.) ve Un kurdu (Tenebrio molitor Ludwig) üzerinde incelenmiştir. Bacillus cereus izolatları bu zararlı böcekler üzerinde en yüksek etkiyi göstermiş ve hedeflenen zararlıların biyolojik mücadelesinde kullanılabilecek potansiyel bir etmen olarak saptanmıştır.

Keywords

Capnodis tenebrionis, bakteriyel izolatlar, biyolojik kontrol

The isolation and characterization of bacteria isolated from Capnodis tenebrionis L. and their biological control potential against some insect pests

Abstract

Capnodis tenebrionis L. (Coleoptera: Buprestidae) is one of the most destructive pests of stone fruit orchards in several countries, including Türkiye. In the present study, 21 bacterial isolates were isolated from the larvae and adults of C. tenebrionis. The morphological, physiological, biochemical and molecular characteristics of the bacterial isolates were determined. The bacterial flora isolated from C. tenebrionis was as follows: Bacillus cereus, B. mycoides, B. pumilus, Paenibacillus xylanilyticus, B. flexus, B. simplex, Raoultella terrigena, Enterobacter cloacae, Klebsiella oxytoca, B. safensis, B. amyloliquefaciens and B. aryabhattai. The biological control potential of these bacterial isolates against the larvae of several economic pest species, the elm leaf beetle (Pyrrhalta luteola (M.)), the honeycomb moth (Galleria mellonella L.), and the mealworm (Tenebrio molitor Ludwig), was investigated. The isolates of B. cereus showed the highest efficacy against these insect pests.

Keywords

Capnodis tenebrionis, Bacterial isolates, Biological control

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