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The frequency of respiratory tract viral agents in children with lower respiratory tract infections

Year 2013, Volume: 48 Issue: 3, 215 - 220, 01.10.2013

Abstract

Aim: Lower respiratory tract infections LRTI have high morbidity rates in children In this study it was aimed to investigate the prevalence of respiratory viruses in children with LRTI symptoms Material and Method: A total of 160 children who were diagnosed with LRTI between October 2009 and March 2010 were included in the study The presence of respiratory syncytial virus RSV A B influenza virus A B parainfluenza virus PIV 1 2 3 4 human metapneumovirus rhinovirus and coronavirus OC43 229E in throat swab samples were investigated by real time PCR The RealAccurateTM Respiratory RT PCR Kit PathoFinder B V Netherlands Results: In 67 samples 41 8 at least one virus which could cause acute respiratory tract infection was found Overall RSV was the most frequently identified virus 52 2 followed by rhinovirus 26 8 coronavirus 5 metapneumovirus 2 9 and PIV 1 1 4 As the other viral agents coronavirus was detected in 4 samples 5 hMPV was detected in 2 samples 2 9 and PIV was detected in 1 sample 1 4 When the frequency of coinfections was evaluated RSV Rhinovirus association was found in 4 samples RSV Coronavirus association was found in 1 sample Rhinovirus Coronavirus association was found in 1 sample and RSV Rhinovirus Coronavirus association was found in 1 sample Conclusions: In 41 8 of the study group a viral factor responsible for the clinical signs was detected For that reason rapid and sensitive diagnosis of viruses which lead to respiratory infections will guide the clinician for avoidance of redundant antibiotic therapy and preventing viral hospital infections Turk Arch Ped 2013; 48: 215 220

References

  • Akşit S. Acute respiratory tract infections-1. STED 2002; 11: 132
  • Centers for Disease Control and Prevention. Prevention and Control of influenza: recommendations of the Immunization Practices Advisory Committee (ACIP). MMWR Morb Mortal Wkly Rep 1995; 44: 1-22.
  • Yolken HY. Laboratory diagnosis of viral infections. In: Galasso GJ, Whitley RJ, Merigan TC, (eds). Practical diagnosis of viral infections. New York, 1993: 17-25.
  • Liolios L, Jenney A, Spelman D, Kotsimbos T, Catton M, Wesselingh S. Comparison of a multiplex reverse transcriptionPCR-enzyme hybridization assay with conventional viral culture and immunofluorescence techniques for the detection of seven viral respiratory pathogens. J Clin Microbiol 2001; 39(8): 2779-83.
  • Berman S. Epidemiology of acute respiratory infections in children of developing countries. Rev Infect Dis 1991; 13(Suppl 6): 454-62.
  • Suwanjutha S, Chantarojanasiri T, Watthana-kasetr S, et al. A study of nonbacterial agents of acute lower respiratory tract infection in Thai children. Rev Infect Dis 1990; 12(Suppl 8): 923-8.
  • Simoes EAF, Cherian T, Chow J, Shahid-Salles SA, Laxminarayan R, John TJ. Acute respiratory ınfections in children. In: Jamison DT, Breman JG, Measham AR, (eds). Disease control priorities in developing countries. 2nd edt. Washington (DC): World Bank, 200 Chapter 25. Ieven M, Goossens H. Relevance of nucleic acid amplification techniques for diagnosis of respiratory tract infections in the clinical laboratory. Clin Microbiol Rev 1997; 10(2): 242-56.
  • Mahony JB, Blackhouse G, Babwah J, et al. Cost analysis of multiplex PCR testing for diagnosing respiratory virus infections. J Clin Microbiol 2009; 47(9): 2812-7.
  • Mahony JB. Detection of respiratory viruses by molecular methods. Clin Microbiol Rev 2008; 21: 716-47.
  • Sancaklı Ö, Yenigün A, Kırdar S. Alt solunum yolu enfeksiyonunda nazofaringeal örneklerde polimeraz zincir reaksiyonu sonuçları. J Pediatr Inf 2012; 6: 84-9.
  • Yüksel H, Yilmaz O, Akçali S, et al. Common viral etiologies of community acquired lower respiratory tract infections in young children and their relationship with long term complications. Mikrobiyol Bul 2008; 42(3): 429-35.
  • Brief report: Respiratory syncytial virus activity-United States, 2004-2005. MMWR 2005; 54; 1259-60.
  • Sözeri B, Gülen F, Demir E, Tanaç R, Altınöz S, Zeyrek CD. Parainfluenza virus in frequently recurrent wheezing. Turkiye Klinikleri J Pediatr 2009; 18: 181-5.
  • Karaman Ö, Tatlı Güneş B, Erbayraktar Z, et al. Recurrence of wheezing episodes in children with respiratory syncytial virus and non-respiratory syncytial virus bronchiolitis. Turkiye Klinikleri J Med Sci 2011; 31: 1507-13.
  • Tanır G, Doğru Ü, Uzunali Ö, Akar N. Frequency and clinical features of respiratory syncytial virus (RSV) infections in infants with lower respiratory tract infection. T Klin Pediatri 2000; 9: 93-7.
  • Yılmaz G, Uzel N, Isık N, Baysal SU, Aslan S, Badur S. Viral lower respiratory tract infections in children in İstanbul, Turkey. Ped Infect Dis J 1999; 18: 173.
  • Kanra G, Tezcan S, Yılmaz G, Turkish National Respiratory Syncytial Virus (RSV) Team. Respiratory syncytial virus epidemiology in Turkey, Turk J Pediatr 2005; 47; 303-8.
  • Wang W, Cavailler P, Ren P, et al. Molecular monitoring of causative viruses in child acute respiratory infection in endemo-epidemic situations in Shanghai. J Clin Virol 2010; 49: 211-8.
  • Do AH, van Doorn HR, Nghiem MN, et al. Viral etiologies of acute respiratory infections among hospitalized Vietnamese children in Ho Chi Minh City, 2004-2008. PLoS One 2011;6(3): 18176.
  • Bezerra PG, Britto MC, Correia JB, et al. Viral and atypical bacterial detection in acute respiratory infection in children under five years. PLoS One 2011; 6(4): 18928.
  • Loens K, Van Heirstraeten L, Malhotra-Kumar S, Goossens H, Ieven M. Optimal sampling sites and methods for detection of pathogens possibly causing community-acquired lower respiratory tract infections. J Clin Microbiol 2009; 47: 21-31.
  • Özyörük D. Respiratory syncytial virus infections in childhood. STED 2003: 12: 94-6.
  • Frobert E, Escuret V, Javouhey E, et al. Respiratory viruses in children admitted to hospital intensive care units: evaluating the CLART® Pneumovir DNA array. J Med Virol 2011; 83: 150-5.

Alt solunum yolu enfeksiyonu olan çocuklarda solunum yolu viral etkenlerinin sıklığı

Year 2013, Volume: 48 Issue: 3, 215 - 220, 01.10.2013

Abstract

Amaç: Alt solunum yolu enfeksiyonları çocuklarda sık görülür ve özellikle erken çocukluk çağında ölüm oranı yüksektir Bu araştırmada bölgemizde alt solunum yolu enfeksiyonları belirtileri bulunan çocuklarda solunum yolu viral etkenlerinin sıklığının belirlenmesi amaçlanmıştır.

Gereç ve Yöntem: Çalışma grubu Ekim 2009 Mart 2010 tarihleri arasında alt solunum yolu enfeksiyonları belirtileri ile hastaneye başvuran 160 çocuktan ort 14 6 ay oluşturulmuştur Bu çocuklardan alınan boğaz sürüntü örneklerinde respiratuar sinsityal virüs A B influenza virüs A B parainfluenza virüs 1 2 3 4 insan metapnömovirüs rinovirüs ve koronavirüs OC43 229E varlığı real time PCR yöntemiyle [The RealAccurateTM Respiratory RT PCR Kit PathoFinder BV Hollanda ] araştırılmıştır.

Bulgular: Örneklerin 67 rsquo;sinde 41 8 alt solunum yolu enfeksiyonları belirtilerine neden olabilecek bir viral etken saptanmıştır Respiratuar sinsityal virüs 61 2 rsquo;lik oranla 41 67 en sık saptanan virüs olmuştur Bunu 35 8 24 67 ile rinovirüsler izlemektedir Dört örnekte 5 koronavirüs iki örnekte 2 9 ldquo;insan metapnömovirüs rdquo; bir örnekte de 1 4 parainfluenza virüs diğer viral etkenler olarak belirlenmiştir Birlikte enfeksiyon sıklıkları değerlendirildiğinde dört örnekte respiratuar sinsityal virüs rinovirüs birlikteliği bir örnekte respiratuar sinsityal virüs koronavirüs birlikteliği bir örnekte rinovirüs koronavirüs birlikteliği bir örnekte de respiratuar sinsityal virüs rinovirüs koronavirüs birlikteliği saptanmıştır. 

Çıkarımlar: Çalışma grubunun 41 8 rsquo;inde klinik belirtilerden sorumlu olabilecek bir viral etken saptanmıştır Bu nedenle viral solunum yolu enfeksiyonlarına neden olan virüslerin hızlı ve duyarlı tanısı gereksiz antibiyotik kullanımının engellenebilmesi ve bu virüslerin neden olabilecekleri hastane enfeksiyonlarının önlenebilmesi açısından klinisyene yol gösterici olacaktır.

References

  • Akşit S. Acute respiratory tract infections-1. STED 2002; 11: 132
  • Centers for Disease Control and Prevention. Prevention and Control of influenza: recommendations of the Immunization Practices Advisory Committee (ACIP). MMWR Morb Mortal Wkly Rep 1995; 44: 1-22.
  • Yolken HY. Laboratory diagnosis of viral infections. In: Galasso GJ, Whitley RJ, Merigan TC, (eds). Practical diagnosis of viral infections. New York, 1993: 17-25.
  • Liolios L, Jenney A, Spelman D, Kotsimbos T, Catton M, Wesselingh S. Comparison of a multiplex reverse transcriptionPCR-enzyme hybridization assay with conventional viral culture and immunofluorescence techniques for the detection of seven viral respiratory pathogens. J Clin Microbiol 2001; 39(8): 2779-83.
  • Berman S. Epidemiology of acute respiratory infections in children of developing countries. Rev Infect Dis 1991; 13(Suppl 6): 454-62.
  • Suwanjutha S, Chantarojanasiri T, Watthana-kasetr S, et al. A study of nonbacterial agents of acute lower respiratory tract infection in Thai children. Rev Infect Dis 1990; 12(Suppl 8): 923-8.
  • Simoes EAF, Cherian T, Chow J, Shahid-Salles SA, Laxminarayan R, John TJ. Acute respiratory ınfections in children. In: Jamison DT, Breman JG, Measham AR, (eds). Disease control priorities in developing countries. 2nd edt. Washington (DC): World Bank, 200 Chapter 25. Ieven M, Goossens H. Relevance of nucleic acid amplification techniques for diagnosis of respiratory tract infections in the clinical laboratory. Clin Microbiol Rev 1997; 10(2): 242-56.
  • Mahony JB, Blackhouse G, Babwah J, et al. Cost analysis of multiplex PCR testing for diagnosing respiratory virus infections. J Clin Microbiol 2009; 47(9): 2812-7.
  • Mahony JB. Detection of respiratory viruses by molecular methods. Clin Microbiol Rev 2008; 21: 716-47.
  • Sancaklı Ö, Yenigün A, Kırdar S. Alt solunum yolu enfeksiyonunda nazofaringeal örneklerde polimeraz zincir reaksiyonu sonuçları. J Pediatr Inf 2012; 6: 84-9.
  • Yüksel H, Yilmaz O, Akçali S, et al. Common viral etiologies of community acquired lower respiratory tract infections in young children and their relationship with long term complications. Mikrobiyol Bul 2008; 42(3): 429-35.
  • Brief report: Respiratory syncytial virus activity-United States, 2004-2005. MMWR 2005; 54; 1259-60.
  • Sözeri B, Gülen F, Demir E, Tanaç R, Altınöz S, Zeyrek CD. Parainfluenza virus in frequently recurrent wheezing. Turkiye Klinikleri J Pediatr 2009; 18: 181-5.
  • Karaman Ö, Tatlı Güneş B, Erbayraktar Z, et al. Recurrence of wheezing episodes in children with respiratory syncytial virus and non-respiratory syncytial virus bronchiolitis. Turkiye Klinikleri J Med Sci 2011; 31: 1507-13.
  • Tanır G, Doğru Ü, Uzunali Ö, Akar N. Frequency and clinical features of respiratory syncytial virus (RSV) infections in infants with lower respiratory tract infection. T Klin Pediatri 2000; 9: 93-7.
  • Yılmaz G, Uzel N, Isık N, Baysal SU, Aslan S, Badur S. Viral lower respiratory tract infections in children in İstanbul, Turkey. Ped Infect Dis J 1999; 18: 173.
  • Kanra G, Tezcan S, Yılmaz G, Turkish National Respiratory Syncytial Virus (RSV) Team. Respiratory syncytial virus epidemiology in Turkey, Turk J Pediatr 2005; 47; 303-8.
  • Wang W, Cavailler P, Ren P, et al. Molecular monitoring of causative viruses in child acute respiratory infection in endemo-epidemic situations in Shanghai. J Clin Virol 2010; 49: 211-8.
  • Do AH, van Doorn HR, Nghiem MN, et al. Viral etiologies of acute respiratory infections among hospitalized Vietnamese children in Ho Chi Minh City, 2004-2008. PLoS One 2011;6(3): 18176.
  • Bezerra PG, Britto MC, Correia JB, et al. Viral and atypical bacterial detection in acute respiratory infection in children under five years. PLoS One 2011; 6(4): 18928.
  • Loens K, Van Heirstraeten L, Malhotra-Kumar S, Goossens H, Ieven M. Optimal sampling sites and methods for detection of pathogens possibly causing community-acquired lower respiratory tract infections. J Clin Microbiol 2009; 47: 21-31.
  • Özyörük D. Respiratory syncytial virus infections in childhood. STED 2003: 12: 94-6.
  • Frobert E, Escuret V, Javouhey E, et al. Respiratory viruses in children admitted to hospital intensive care units: evaluating the CLART® Pneumovir DNA array. J Med Virol 2011; 83: 150-5.
There are 23 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section Review
Authors

Sinem Akçalı This is me

Nisel Yılmaz This is me

Özlem Güler This is me

Tamer Şanlidağ1 This is me

Murat Anıl This is me

Publication Date October 1, 2013
Published in Issue Year 2013 Volume: 48 Issue: 3

Cite

APA Akçalı, S., Yılmaz, N., Güler, Ö., Şanlidağ1, T., et al. (2013). Alt solunum yolu enfeksiyonu olan çocuklarda solunum yolu viral etkenlerinin sıklığı. Türk Pediatri Arşivi, 48(3), 215-220.
AMA Akçalı S, Yılmaz N, Güler Ö, Şanlidağ1 T, Anıl M. Alt solunum yolu enfeksiyonu olan çocuklarda solunum yolu viral etkenlerinin sıklığı. Türk Pediatri Arşivi. October 2013;48(3):215-220.
Chicago Akçalı, Sinem, Nisel Yılmaz, Özlem Güler, Tamer Şanlidağ1, and Murat Anıl. “Alt Solunum Yolu Enfeksiyonu Olan çocuklarda Solunum Yolu Viral Etkenlerinin sıklığı”. Türk Pediatri Arşivi 48, no. 3 (October 2013): 215-20.
EndNote Akçalı S, Yılmaz N, Güler Ö, Şanlidağ1 T, Anıl M (October 1, 2013) Alt solunum yolu enfeksiyonu olan çocuklarda solunum yolu viral etkenlerinin sıklığı. Türk Pediatri Arşivi 48 3 215–220.
IEEE S. Akçalı, N. Yılmaz, Ö. Güler, T. Şanlidağ1, and M. Anıl, “Alt solunum yolu enfeksiyonu olan çocuklarda solunum yolu viral etkenlerinin sıklığı”, Türk Pediatri Arşivi, vol. 48, no. 3, pp. 215–220, 2013.
ISNAD Akçalı, Sinem et al. “Alt Solunum Yolu Enfeksiyonu Olan çocuklarda Solunum Yolu Viral Etkenlerinin sıklığı”. Türk Pediatri Arşivi 48/3 (October 2013), 215-220.
JAMA Akçalı S, Yılmaz N, Güler Ö, Şanlidağ1 T, Anıl M. Alt solunum yolu enfeksiyonu olan çocuklarda solunum yolu viral etkenlerinin sıklığı. Türk Pediatri Arşivi. 2013;48:215–220.
MLA Akçalı, Sinem et al. “Alt Solunum Yolu Enfeksiyonu Olan çocuklarda Solunum Yolu Viral Etkenlerinin sıklığı”. Türk Pediatri Arşivi, vol. 48, no. 3, 2013, pp. 215-20.
Vancouver Akçalı S, Yılmaz N, Güler Ö, Şanlidağ1 T, Anıl M. Alt solunum yolu enfeksiyonu olan çocuklarda solunum yolu viral etkenlerinin sıklığı. Türk Pediatri Arşivi. 2013;48(3):215-20.