EFFECT OF BITTER MELON AGAINST CISPLATIN AND VALPROIC ACID-INDUCED GENOTOXICITY IN Drosophila melanogaster Meigen

Çağla Ersöz; Deniz Altun Çolak

doi:10.23902/trkjnat.1302555

Research Article

EFFECT OF BITTER MELON AGAINST CISPLATIN AND VALPROIC ACID-INDUCED GENOTOXICITY IN Drosophila melanogaster Meigen

Year 2023, Volume: 24 Issue: 2, 57 - 66, 15.10.2023

Çağla Ersöz Deniz Altun Çolak

https://doi.org/10.23902/trkjnat.1302555

Abstract

In human body, which is exposed to number of chemicals and physical agents in daily life, malfunctions may occur from time to time in detoxification mechanisms that will prevent the harmful effects of genotoxic agents, whose negative effects cannot be eliminated. In this study, the genoprotective effects of bitter melon (Momordica charantia L.) seed and fruit extracts against the genotoxic potential of the antineoplastic agent cisplatin and the antiepileptic drug valproic acid in 72±4 hours old transheterozygous larvae of the fruit fly Drosophila melanogaster Meigen were determined by the Somatic Mutation and Recombination Test (SMART). The results showed that the mutation frequencies approached the negative control values by inhibiting the formation of chemical agent-induced mutant clones in all doses in the experimental groups in which plant extracts were applied at doses of 1.25, 2.5 ve 5 mg/mL, while the mutation frequency increased only in the groups that applied cisplatin and valproic acid. These results show that co-administration of cisplatin and valproic acid reduces the genotoxic effect of M. charantia.

Keywords

Genotoxic effect, Momordica charantia, Somatic mutation, Recombination Test

Project Number

FYL-2018-560

References

1. Abbas, Z. & Rehman, S. 2018. An overview of cancer treatment modalities. Neoplasm, 1: 139-157. http://dx.doi.org/10.5772/intechopen.76558
2. Adewole, K.E., Attah, A.F. & Osawe, S.O. 2021. Exploring phytotherapeutic approach in the management of valproic acid-induced toxicity. Advances in Traditional Medicine, 23: 347-367. https://doi.org/10.1007/s13596-021-00575-6
3. Amkiss, S., Dalouh, A. & Idaomar, M. 2021. Chemical composition, genotoxicity and antigenotoxicity study of Artemisia herba-alba using the eye and wing SMART assay of Drosophila melanogaster. Arabian Journal of Chemistry, 4(3): 102976. https://doi.org/10.1016/j.arabjc.2020.102976
4. Anilakumar, K.R., Kumar, G.P. & Ilaiyaraja, N. 2015. Nutritional, pharmacological and medicinal properties of Momordica charantia. International Journal of Nutrition and Food Sciences, 4(1): 75-83.
5. Ateşci, F.C., Oğuzhanoğlu, N.K., Baltalarli, B., Karadağ, F., Ozdel, O. & Karagöz, N. 2003. Psychiatric disorders in cancer patients and associated factors. Turkish Journal of Psychiatry, 14(2): 145-152.
6. Aydın, Ş. & Sohtaoğlu, M. 2015. Neurologic problems accompanying lung cancer. Bulletin of Thoracic Surgery, 9(4): 307-313. https://doi.org/10.5152/tcb.2015.076
7. Becit, M., Dilsiz, S.A. & Başaran, N. 2021. Effects of pycnogenol and its combinations with cisplatin on hepatocellular carcinoma cell viability. FABAD Journal of Pharmaceutical Sciences, 46(1): 13-22.
8. Cauli, O. 2021. Oxidative stress and cognitive alterations induced by cancer chemotherapy drugs: A scoping review. Antioxidants, 10(7): 1116. https://doi.org/10.3390/antiox10071116
9. Chateauvieux, S., Morceau, F., Dicato, M. & Diederich, M. 2010. Molecular and therapeutic potential and toxicity of valproic acid. Journal of Biomedicine and Biotechnology, 2010: 1-18. https://doi.org/10.1155/2010/479364
10. Chekka, S.V. & Mantipelly, N.K. 2020. Momordica charantia: A natural medicinal plant. GSC Biological and Pharmaceutical Sciences, 12(2): 129-135. https://doi.org/10.30574/gscbps.2020.12.2.0251
11. Christen, W.G., Gaziano, J.M. & Hennekens, C.H. 2000. Design of Physicians' Health Study II-a randomized trial of beta-carotene, vitamins E and C, and multivitamins, in prevention of cancer, cardiovascular disease, and eye disease, and review of results of completed trials. Annals of Epidemiology, 10(2): 125-134.
12. Çolak, D.A. & Uysal, H. 2021. Determination of the genotoxic effects of various dioxins by Drosophila wing spot test. Indian Journal of Experimental Biology, 59(09): 640-645.
13. Dasari, S. & Tchounwou, P.B. 2014. Cisplatin in cancer therapy: molecular mechanisms of action. European Journal of Pharmacology, 740: 364-378. https://doi.org/10.1016/j.ejphar.2014.07.025
14. Denli, M., Aydin, H.I., Döndaröz, R., Özişik, T., Erdem, E. & Baltaci, V. 2000. Genotoxicity evaluation in female patients on valproic acid monotherapy using alkaline single cell gel electrophoresis (comet assay). Eastern Journal of Medicine, 5(2): 61-65.
15. Dillard, C.J. & German, J.B. 2000. Phytochemicals: nutraceuticals and human health. Journal of the Science of Food and Agriculture, 80(12): 1744-1756.
16. Duarte, D., Cardoso, A. & Vale, N. 2021. Synergistic growth inhibition of HT-29 colon and MCF-7 breast cancer cells with simultaneous and sequential combinations of antineoplastics and CNS drugs. International Journal of Molecular Sciences, 22(14): 7408. https://doi.org/10.3390/ijms22147408
17. Dugbartey, G.J., Peppone, L.J. & de Graaf, I.A. 2016. An integrative view of cisplatin-induced renal and cardiac toxicities: Molecular mechanisms, current treatment challenges and potential protective measures. Toxicology, 371: 58-66. https://doi.org/10.1016/j.tox.2016.10.001
18. Ersöz, Ç. & Çolak, D.A. 2019. The effect of bitter melon against cisplatin and valproic acid-induced toxicity on the percentage of survival and lifespan in Drosophila melanogaster. Eurasian Journal of Biological and Chemical Sciences, 2(2): 73-78.
19. Fiest, K.M., Sauro, K.M., Wiebe, S., Patten, S.B., Kwon, C.S., Dykeman, J., Pringsheim, T., Lorenzetti, D.L. & Jetté, N. 2017. Prevalence and incidence of epilepsy: a systematic review and meta-analysis of international studies. Neurology, 88(3): 296-303. https://doi.org/10.1212/WNL.0000000000003509
20. Frei, H. & Würgler, F.E. 1988. Statistical methods to decide whether mutagenicity test data from Drosophila assays indicate a positive, negative, or inconclusive result. Mutation Research/Environmental Mutagenesis and Related Subjects, 203(4): 297-308.
21. Frölich, A. & Würgler, F.E. 1990. Drosophila wing-spot test: improved detectability of genotoxicity of polycyclic aromatic hydrocarbons. Mutation Research/Environmental Mutagenesis and Related Subjects, 234(2): 71-80.
22. Gaitatzis, A. & Sander, J.W. 2013. The long-term safety of antiepileptic drugs. CNS Drugs, 27(6): 435-455.
23. Gomez-Ruiz, S., Maksimović-Ivanić, D., Mijatović, S. & Kaluđerović, G.N. 2012. On the discovery, biological effects, and use of cisplatin and metallocenes in anticancer chemotherapy. Bioinorganic Chemistry and Applications, 2012.
24. Graf, U., Würgler, F.E., Katz, A.J., Frei, H., Juon, H., Hall, C.B. & Kale, P.G. 1984. Somatic mutation and recombination test in Drosophila melanogaster. Environmental Mutagenesis, 6(2): 153-188.
25. Graf, U., Frei, H., Kägi, A., Katz, A.J. & Würgler, F.E. 1989. Thirty compounds tested in the Drosophila wing spot test. Mutation Research/Genetic Toxicology, 222(4): 359-373.
26. Gunjan, M., Naing, T.W., Saini, R.S., Ahmad, A., Naidu, J.R. & Kumar, I. 2015. Marketing trends & future prospects of herbal medicine in the treatment of various disease. World Journal of Pharmaceutical Research, 4(9): 132-155.
27. Guterres, Z.R., Zanetti, T.A., Sennes-Lopes, T.F. & Gomes da Silva, A.F. 2015. Genotoxic and antigenotoxic potential of Momordica charantia Linn (Cucurbitaceae) in the wing spot test of Drosophila melanogaster. Journal of Medicinal Food, 18(10): 1136-1142. https://doi.org/10.1089/jmf.2014.0099
28. Idaomar, M., El Hamss, R., Bakkali, F., Mezzoug, N., Zhiri, A., Baudoux, D., Munoz-Serrano, A., Liemans, V. & Alonso-Moraga, A. 2002. Genotoxicity and antigenotoxicity of some essential oils evaluated by wing spot test of Drosophila melanogaster. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 513(1-2): 61-68.
29. Kastenbaum, M.A. & Bowman, K.O. 1970. Tables for determining the statistical significance of mutation frequencies. Mutation Research, 9: 527-549.
30. Katz, A.J. 1987. Genotoxic effects of cisplatin in somatic tissue of Drosophila melanogaster. Environmental and Molecular Mutagenesis, 10(2): 197-203.
31. Khalid, Z., Hassan, S.M., Mughal, S.S., Hassan, S.K. & Hassan, H. 2021a. Phenolic profile and biological properties of Momordica charantia. Chemical and Biomolecular Engineering, 6(1): 17. https://doi.org/10.11648/j.cbe.20210601.13
32. Khalid, Z., Hassan, S., Shahzad, S. & Khurram, H. 2021b. A review on biological attributes of Momordica charantia. Advances in Bioscience and Bioengineering, 9(1): 8-12. https://doi.org/10.11648/j.abb.20210901.12
33. Lheureux, P.E. & Hantson, P. 2009. Carnitine in the treatment of valproic acid-induced toxicity. Clinical Toxicology, 47(2): 101-111.
34. Li, Z., Xia, A., Li, S., Yang, G., Jin, W., Zhang, M. & Wang, S. 2020. The pharmacological properties and therapeutic use of bitter melon (Momordica charantia L.). Current Pharmacology Reports, 6(3): 103-109. https://doi.org/10.1007/s40495-020-00219-4
35. Marsh, J.L. & Thompson, L.M. 2006. Drosophila in the study of neurodegenerative disease. Neuron, 52(1): 169-178. https://doi.org/10.1016/j.neuron.2006.09.025
36. Minagawa, H., Uchitomi, Y., Yamawaki, S. & Ishitani, K. 1996. Psychiatric morbidity in terminally III cancer patients: A prospective study. Cancer: Interdisciplinary International Journal of the American Cancer Society, 78(5): 1131-1137.
37. Mollet, P. & Würgler, F.E. 1974. Detection of somatric recombination and mutation in Drosophila a method for testing genetic activity of chemical compounds. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 25(3): 421-424.
38. Nanau, R.M. & Neuman, M.G. 2013. Adverse drug reactions induced by valproic acid. Clinical Biochemistry, 46(15): 1323-1338.
39. Nenclares, P. & Harrington, K.J. 2020. The biology of cancer. Medicine, 48(2): 67-72. https://doi.org/10.1016/j.mpmed.2019.11.001
40. Nerurkar, P.V., Johns, L.M., Buesa, L.M., Kipyakwai, G., Volper, E., Sato, R., Shah, P., Feher, D., Williams, P.G. & Nerurkar, V.R. 2011. Momordica charantia (bitter melon) attenuates high-fat diet-associated oxidative stress and neuroinflammation. Journal of Neuroinflammation. 8: 1-19. https://doi.org/10.1186/1742-2094-8-64
41. Olusanya, T.O., Haj Ahmad, R.R., Ibegbu, D.M., Smith, J.R. & Elkordy, A.A. 2018. Liposomal drug delivery systems and anticancer drugs. Molecules. 23(4): 907. https://doi.org/10.3390/molecules23040907
42. Ornoy, A., Becker, M., Weinstein-Fudim, L. & Ergaz, Z. 2020. S-adenosine methionine (SAME) and valproic acid (VPA) as epigenetic modulators: Special emphasis on their interactions affecting nervous tissue during pregnancy, International Journal of Molecular Sciences. 21(10): 3721. https://doi.org/10.3390/ijms21103721
43. Pal, R., Kumar, B., Akhtar, M.J. & Chawla, P.A. 2021. Voltage gated sodium channel inhibitors as anticonvulsant drugs: A systematic review on recent developments and structure activity relationship studies. Bioorganic Chemistry, 115: 105230. https://doi.org/10.1016/j.bioorg.2021.105230
44. Paul, A., Bandyopadhyay, S., Acharyya, P. & Raychaudhuri, S.S. 2010. Studies on genetic diversity of twelve accessions of Momordica charantia L. using morphological, RAPD and SCAR Markers. Asian Journal of Plant Sciences, 9(8): 471.
45. Poovitha, S. & Parani, M. 2020. Protein extracts from Momordica charantia var. charantia and M. charantia var. muricata show anti‐lipidemic and antioxidant properties in experimental type 2 diabetic rats, Journal of Food Biochemistry, 44(9): e13370. https://doi.org/10.1111/jfbc.13370
46. Prakash, S., Kumar, M., Kumari, N., Thakur, M., Rathour, S., Pundir, A., Kumar, S.A., Punia, S.B., Dhumal, S., Singh, S., Thiyagarajan, A., Sharma, A., Sharma, M., Changan, S., Sasi, M., Senapathy, M., Pradhan, P.C., Garg, N.T., Tamilsevan, I., Nitin M., Abdel- Daim, M.M., Puri, S., Natta, S., Dey, A., Amarowicz, R. & Mekhemar, M. 2021. Plant-Based Antioxidant Extracts and Compounds in the Management of Oral Cancer, Antioxidants, 10(9): 1358. https://doi.org/10.3390/antiox10091358
47. Rashid, A., Aqeel, M., Malik, B. & Salim, D.S. 2021. The prevalence of psychiatric disorders in breast cancer patients; a cross-sectional study of breast cancer patients experience in Pakistan, Nature-Nurture Journal of Psychology, 1(1): 1-7.
48. Sarıkaya, R. & Çakır, Ş. 2005. Genotoxicity testing of four food preservatives and their combinations in the Drosophila wing spot test. Environmental Toxicology and Pharmacology, 20(3): 424-430. https://doi.org/10.1016/j.etap.2005.05.002
49. Schneider, D. 2000. Using Drosophila as a model insect. Nature Reviews Genetics, 1(3): 218-226. https://doi.org/10.1038/35042080
50. Shona, S.I., Rizk, A.A., El Sadik, A.O., Emam, H.Y. & Ali, E.N. 2018. Effect of valproic acid administration during pregnancy on postnatal development of cerebellar cortex and the possible protective role of folic acid. Folia Morphologica, 77(2): 201-209. https://doi.org/10.5603/FM.a2017.0100
51. Singh, D., Gupta, S., Verma, I., Morsy, M.A., Nair, A.B. & Ahmed, A.S.F. 2021. Hidden pharmacological activities of valproic acid: A new insight. Biomedicine & Pharmacotherapy, 142: 112021. https://doi.org/10.1016/j.biopha.2021.112021
52. Stein, K.D., Syrjala, K.L. & Andrykowski, M.A. 2008. Physical and psychological long‐term and late effects of cancer. Cancer, 112(S11): 2577-2592. https://doi.org/10.1002/cncr.23448
53. Szabad, J., Soós, I., Polgár, G. & Héjja, G. 1983. Testing the mutagenicity of malondialdehyde and formaldehyde by the Drosophila mosaic and the sex-linked recessive lethal tests. Mutation Research/Environmental Mutagenesis and Related Subjects, 113(2): 117-133.
54. Yavarpour-Bali, H., Ghasemi-Kasman, M. & Pirzadeh, M. 2019. Curcumin-loaded nanoparticles: A novel therapeutic strategy in treatment of central nervous system disorders. International Journal of Nanomedicine. 14: 4449. https://doi.org/10.2147/IJN.S208332
55. Vale, C.R.D., Silva, C.R., Oliveira, C.M.A.D., Silva, A.L., Carvalho, S.D. & Lee, C.C. 2013. Assessment of toxic, genotoxic, antigenotoxic, and recombinogenic activities of Hymenaea courbaril (Fabaceae) in Drosophila melanogaster and mice. Genetics and Molecular Research, 12(3): 2712-2724. http://dx.doi.org/10.4238/2013.July.30.9
56. Würgler, F.E. 1986. Mutagenicity assays detecting recombination. Progress in Clinical and Biological Research. 209: 85-90.
57. Zhuo, C., Xun, Z., Hou, W., Ji, F., Lin, X., Tian, H., Zheng, W., Chen, M., Liu, C., Wang, W. & Chen, C. 2019. Surprising anticancer activities of psychiatric medications: old drugs offer new hope for patients with brain cancer, Frontiers in Pharmacology. 10: 1262. https://doi.org/10.3389/fphar.2019.01262
58. Zimmering, S., Cruces, M.P., Pimentel, E., Arceo, C., Carrasco, G. & Olvera, O. 1997. On the recovery of single spots with the flr phenotype in the wing spot test in Drosophila. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 379(1): 77-82. https://doi.org/10.1016/S0027-5107(97)00110-3

Year 2023, Volume: 24 Issue: 2, 57 - 66, 15.10.2023

Çağla Ersöz Deniz Altun Çolak

https://doi.org/10.23902/trkjnat.1302555

Abstract

Günlük hayatta birçok kimyasal ve fiziksel ajana maruz kalan insan vücudunda, genotoksik ajanların zararlı etkilerini önleyecek detoksifikasyon mekanizmalarında zaman zaman bozukluklar oluşabilmekte ve bu ajanların olumsuz etkileri ortadan kaldırılamamaktadır. Bu çalışmada, antineoplastik bir ajan olan cisplatin ve bir antiepileptik ilaç olan valproik asidin 72±4 saatlik Drosophila melanogaster Meigen transheterozigot larvalarındaki genotoksik potansiyeline karşı acı kavun (Momordica charantia L.) tohum ve meyve ekstraktlarının genoprotektif etkileri Somatik Mutasyon ve Rekombinasyon Testi (SMART) ile belirlenmiştir. 1,25; 2,5 ve 5 mg/mL dozlarında bitki ekstraktları uygulanan deney gruplarında, mutasyon frekanslarının tüm dozlarda kimyasal madde kaynaklı mutant klon oluşumunu engelleyerek negatif kontrole yaklaşırken mutasyon frekansının sadece sisplatin ve valproik asit eklenen gruplarda arttığı gözlenmiştir. Bu sonuçlar, sisplatin ve valproik asitin birlikte uygulanmasının M. charantia’nın genotoksik etkisinin azalttığını göstermektedir.

Supporting Institution

Erzincan Binali Yıldırım University Research Foundation

Project Number

FYL-2018-560

References

1. Abbas, Z. & Rehman, S. 2018. An overview of cancer treatment modalities. Neoplasm, 1: 139-157. http://dx.doi.org/10.5772/intechopen.76558
2. Adewole, K.E., Attah, A.F. & Osawe, S.O. 2021. Exploring phytotherapeutic approach in the management of valproic acid-induced toxicity. Advances in Traditional Medicine, 23: 347-367. https://doi.org/10.1007/s13596-021-00575-6
3. Amkiss, S., Dalouh, A. & Idaomar, M. 2021. Chemical composition, genotoxicity and antigenotoxicity study of Artemisia herba-alba using the eye and wing SMART assay of Drosophila melanogaster. Arabian Journal of Chemistry, 4(3): 102976. https://doi.org/10.1016/j.arabjc.2020.102976
4. Anilakumar, K.R., Kumar, G.P. & Ilaiyaraja, N. 2015. Nutritional, pharmacological and medicinal properties of Momordica charantia. International Journal of Nutrition and Food Sciences, 4(1): 75-83.
5. Ateşci, F.C., Oğuzhanoğlu, N.K., Baltalarli, B., Karadağ, F., Ozdel, O. & Karagöz, N. 2003. Psychiatric disorders in cancer patients and associated factors. Turkish Journal of Psychiatry, 14(2): 145-152.
6. Aydın, Ş. & Sohtaoğlu, M. 2015. Neurologic problems accompanying lung cancer. Bulletin of Thoracic Surgery, 9(4): 307-313. https://doi.org/10.5152/tcb.2015.076
7. Becit, M., Dilsiz, S.A. & Başaran, N. 2021. Effects of pycnogenol and its combinations with cisplatin on hepatocellular carcinoma cell viability. FABAD Journal of Pharmaceutical Sciences, 46(1): 13-22.
8. Cauli, O. 2021. Oxidative stress and cognitive alterations induced by cancer chemotherapy drugs: A scoping review. Antioxidants, 10(7): 1116. https://doi.org/10.3390/antiox10071116
9. Chateauvieux, S., Morceau, F., Dicato, M. & Diederich, M. 2010. Molecular and therapeutic potential and toxicity of valproic acid. Journal of Biomedicine and Biotechnology, 2010: 1-18. https://doi.org/10.1155/2010/479364
10. Chekka, S.V. & Mantipelly, N.K. 2020. Momordica charantia: A natural medicinal plant. GSC Biological and Pharmaceutical Sciences, 12(2): 129-135. https://doi.org/10.30574/gscbps.2020.12.2.0251
11. Christen, W.G., Gaziano, J.M. & Hennekens, C.H. 2000. Design of Physicians' Health Study II-a randomized trial of beta-carotene, vitamins E and C, and multivitamins, in prevention of cancer, cardiovascular disease, and eye disease, and review of results of completed trials. Annals of Epidemiology, 10(2): 125-134.
12. Çolak, D.A. & Uysal, H. 2021. Determination of the genotoxic effects of various dioxins by Drosophila wing spot test. Indian Journal of Experimental Biology, 59(09): 640-645.
13. Dasari, S. & Tchounwou, P.B. 2014. Cisplatin in cancer therapy: molecular mechanisms of action. European Journal of Pharmacology, 740: 364-378. https://doi.org/10.1016/j.ejphar.2014.07.025
14. Denli, M., Aydin, H.I., Döndaröz, R., Özişik, T., Erdem, E. & Baltaci, V. 2000. Genotoxicity evaluation in female patients on valproic acid monotherapy using alkaline single cell gel electrophoresis (comet assay). Eastern Journal of Medicine, 5(2): 61-65.
15. Dillard, C.J. & German, J.B. 2000. Phytochemicals: nutraceuticals and human health. Journal of the Science of Food and Agriculture, 80(12): 1744-1756.
16. Duarte, D., Cardoso, A. & Vale, N. 2021. Synergistic growth inhibition of HT-29 colon and MCF-7 breast cancer cells with simultaneous and sequential combinations of antineoplastics and CNS drugs. International Journal of Molecular Sciences, 22(14): 7408. https://doi.org/10.3390/ijms22147408
17. Dugbartey, G.J., Peppone, L.J. & de Graaf, I.A. 2016. An integrative view of cisplatin-induced renal and cardiac toxicities: Molecular mechanisms, current treatment challenges and potential protective measures. Toxicology, 371: 58-66. https://doi.org/10.1016/j.tox.2016.10.001
18. Ersöz, Ç. & Çolak, D.A. 2019. The effect of bitter melon against cisplatin and valproic acid-induced toxicity on the percentage of survival and lifespan in Drosophila melanogaster. Eurasian Journal of Biological and Chemical Sciences, 2(2): 73-78.
19. Fiest, K.M., Sauro, K.M., Wiebe, S., Patten, S.B., Kwon, C.S., Dykeman, J., Pringsheim, T., Lorenzetti, D.L. & Jetté, N. 2017. Prevalence and incidence of epilepsy: a systematic review and meta-analysis of international studies. Neurology, 88(3): 296-303. https://doi.org/10.1212/WNL.0000000000003509
20. Frei, H. & Würgler, F.E. 1988. Statistical methods to decide whether mutagenicity test data from Drosophila assays indicate a positive, negative, or inconclusive result. Mutation Research/Environmental Mutagenesis and Related Subjects, 203(4): 297-308.
21. Frölich, A. & Würgler, F.E. 1990. Drosophila wing-spot test: improved detectability of genotoxicity of polycyclic aromatic hydrocarbons. Mutation Research/Environmental Mutagenesis and Related Subjects, 234(2): 71-80.
22. Gaitatzis, A. & Sander, J.W. 2013. The long-term safety of antiepileptic drugs. CNS Drugs, 27(6): 435-455.
23. Gomez-Ruiz, S., Maksimović-Ivanić, D., Mijatović, S. & Kaluđerović, G.N. 2012. On the discovery, biological effects, and use of cisplatin and metallocenes in anticancer chemotherapy. Bioinorganic Chemistry and Applications, 2012.
24. Graf, U., Würgler, F.E., Katz, A.J., Frei, H., Juon, H., Hall, C.B. & Kale, P.G. 1984. Somatic mutation and recombination test in Drosophila melanogaster. Environmental Mutagenesis, 6(2): 153-188.
25. Graf, U., Frei, H., Kägi, A., Katz, A.J. & Würgler, F.E. 1989. Thirty compounds tested in the Drosophila wing spot test. Mutation Research/Genetic Toxicology, 222(4): 359-373.
26. Gunjan, M., Naing, T.W., Saini, R.S., Ahmad, A., Naidu, J.R. & Kumar, I. 2015. Marketing trends & future prospects of herbal medicine in the treatment of various disease. World Journal of Pharmaceutical Research, 4(9): 132-155.
27. Guterres, Z.R., Zanetti, T.A., Sennes-Lopes, T.F. & Gomes da Silva, A.F. 2015. Genotoxic and antigenotoxic potential of Momordica charantia Linn (Cucurbitaceae) in the wing spot test of Drosophila melanogaster. Journal of Medicinal Food, 18(10): 1136-1142. https://doi.org/10.1089/jmf.2014.0099
28. Idaomar, M., El Hamss, R., Bakkali, F., Mezzoug, N., Zhiri, A., Baudoux, D., Munoz-Serrano, A., Liemans, V. & Alonso-Moraga, A. 2002. Genotoxicity and antigenotoxicity of some essential oils evaluated by wing spot test of Drosophila melanogaster. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 513(1-2): 61-68.
29. Kastenbaum, M.A. & Bowman, K.O. 1970. Tables for determining the statistical significance of mutation frequencies. Mutation Research, 9: 527-549.
30. Katz, A.J. 1987. Genotoxic effects of cisplatin in somatic tissue of Drosophila melanogaster. Environmental and Molecular Mutagenesis, 10(2): 197-203.
31. Khalid, Z., Hassan, S.M., Mughal, S.S., Hassan, S.K. & Hassan, H. 2021a. Phenolic profile and biological properties of Momordica charantia. Chemical and Biomolecular Engineering, 6(1): 17. https://doi.org/10.11648/j.cbe.20210601.13
32. Khalid, Z., Hassan, S., Shahzad, S. & Khurram, H. 2021b. A review on biological attributes of Momordica charantia. Advances in Bioscience and Bioengineering, 9(1): 8-12. https://doi.org/10.11648/j.abb.20210901.12
33. Lheureux, P.E. & Hantson, P. 2009. Carnitine in the treatment of valproic acid-induced toxicity. Clinical Toxicology, 47(2): 101-111.
34. Li, Z., Xia, A., Li, S., Yang, G., Jin, W., Zhang, M. & Wang, S. 2020. The pharmacological properties and therapeutic use of bitter melon (Momordica charantia L.). Current Pharmacology Reports, 6(3): 103-109. https://doi.org/10.1007/s40495-020-00219-4
35. Marsh, J.L. & Thompson, L.M. 2006. Drosophila in the study of neurodegenerative disease. Neuron, 52(1): 169-178. https://doi.org/10.1016/j.neuron.2006.09.025
36. Minagawa, H., Uchitomi, Y., Yamawaki, S. & Ishitani, K. 1996. Psychiatric morbidity in terminally III cancer patients: A prospective study. Cancer: Interdisciplinary International Journal of the American Cancer Society, 78(5): 1131-1137.
37. Mollet, P. & Würgler, F.E. 1974. Detection of somatric recombination and mutation in Drosophila a method for testing genetic activity of chemical compounds. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 25(3): 421-424.
38. Nanau, R.M. & Neuman, M.G. 2013. Adverse drug reactions induced by valproic acid. Clinical Biochemistry, 46(15): 1323-1338.
39. Nenclares, P. & Harrington, K.J. 2020. The biology of cancer. Medicine, 48(2): 67-72. https://doi.org/10.1016/j.mpmed.2019.11.001
40. Nerurkar, P.V., Johns, L.M., Buesa, L.M., Kipyakwai, G., Volper, E., Sato, R., Shah, P., Feher, D., Williams, P.G. & Nerurkar, V.R. 2011. Momordica charantia (bitter melon) attenuates high-fat diet-associated oxidative stress and neuroinflammation. Journal of Neuroinflammation. 8: 1-19. https://doi.org/10.1186/1742-2094-8-64
41. Olusanya, T.O., Haj Ahmad, R.R., Ibegbu, D.M., Smith, J.R. & Elkordy, A.A. 2018. Liposomal drug delivery systems and anticancer drugs. Molecules. 23(4): 907. https://doi.org/10.3390/molecules23040907
42. Ornoy, A., Becker, M., Weinstein-Fudim, L. & Ergaz, Z. 2020. S-adenosine methionine (SAME) and valproic acid (VPA) as epigenetic modulators: Special emphasis on their interactions affecting nervous tissue during pregnancy, International Journal of Molecular Sciences. 21(10): 3721. https://doi.org/10.3390/ijms21103721
43. Pal, R., Kumar, B., Akhtar, M.J. & Chawla, P.A. 2021. Voltage gated sodium channel inhibitors as anticonvulsant drugs: A systematic review on recent developments and structure activity relationship studies. Bioorganic Chemistry, 115: 105230. https://doi.org/10.1016/j.bioorg.2021.105230
44. Paul, A., Bandyopadhyay, S., Acharyya, P. & Raychaudhuri, S.S. 2010. Studies on genetic diversity of twelve accessions of Momordica charantia L. using morphological, RAPD and SCAR Markers. Asian Journal of Plant Sciences, 9(8): 471.
45. Poovitha, S. & Parani, M. 2020. Protein extracts from Momordica charantia var. charantia and M. charantia var. muricata show anti‐lipidemic and antioxidant properties in experimental type 2 diabetic rats, Journal of Food Biochemistry, 44(9): e13370. https://doi.org/10.1111/jfbc.13370
46. Prakash, S., Kumar, M., Kumari, N., Thakur, M., Rathour, S., Pundir, A., Kumar, S.A., Punia, S.B., Dhumal, S., Singh, S., Thiyagarajan, A., Sharma, A., Sharma, M., Changan, S., Sasi, M., Senapathy, M., Pradhan, P.C., Garg, N.T., Tamilsevan, I., Nitin M., Abdel- Daim, M.M., Puri, S., Natta, S., Dey, A., Amarowicz, R. & Mekhemar, M. 2021. Plant-Based Antioxidant Extracts and Compounds in the Management of Oral Cancer, Antioxidants, 10(9): 1358. https://doi.org/10.3390/antiox10091358
47. Rashid, A., Aqeel, M., Malik, B. & Salim, D.S. 2021. The prevalence of psychiatric disorders in breast cancer patients; a cross-sectional study of breast cancer patients experience in Pakistan, Nature-Nurture Journal of Psychology, 1(1): 1-7.
48. Sarıkaya, R. & Çakır, Ş. 2005. Genotoxicity testing of four food preservatives and their combinations in the Drosophila wing spot test. Environmental Toxicology and Pharmacology, 20(3): 424-430. https://doi.org/10.1016/j.etap.2005.05.002
49. Schneider, D. 2000. Using Drosophila as a model insect. Nature Reviews Genetics, 1(3): 218-226. https://doi.org/10.1038/35042080
50. Shona, S.I., Rizk, A.A., El Sadik, A.O., Emam, H.Y. & Ali, E.N. 2018. Effect of valproic acid administration during pregnancy on postnatal development of cerebellar cortex and the possible protective role of folic acid. Folia Morphologica, 77(2): 201-209. https://doi.org/10.5603/FM.a2017.0100
51. Singh, D., Gupta, S., Verma, I., Morsy, M.A., Nair, A.B. & Ahmed, A.S.F. 2021. Hidden pharmacological activities of valproic acid: A new insight. Biomedicine & Pharmacotherapy, 142: 112021. https://doi.org/10.1016/j.biopha.2021.112021
52. Stein, K.D., Syrjala, K.L. & Andrykowski, M.A. 2008. Physical and psychological long‐term and late effects of cancer. Cancer, 112(S11): 2577-2592. https://doi.org/10.1002/cncr.23448
53. Szabad, J., Soós, I., Polgár, G. & Héjja, G. 1983. Testing the mutagenicity of malondialdehyde and formaldehyde by the Drosophila mosaic and the sex-linked recessive lethal tests. Mutation Research/Environmental Mutagenesis and Related Subjects, 113(2): 117-133.
54. Yavarpour-Bali, H., Ghasemi-Kasman, M. & Pirzadeh, M. 2019. Curcumin-loaded nanoparticles: A novel therapeutic strategy in treatment of central nervous system disorders. International Journal of Nanomedicine. 14: 4449. https://doi.org/10.2147/IJN.S208332
55. Vale, C.R.D., Silva, C.R., Oliveira, C.M.A.D., Silva, A.L., Carvalho, S.D. & Lee, C.C. 2013. Assessment of toxic, genotoxic, antigenotoxic, and recombinogenic activities of Hymenaea courbaril (Fabaceae) in Drosophila melanogaster and mice. Genetics and Molecular Research, 12(3): 2712-2724. http://dx.doi.org/10.4238/2013.July.30.9
56. Würgler, F.E. 1986. Mutagenicity assays detecting recombination. Progress in Clinical and Biological Research. 209: 85-90.
57. Zhuo, C., Xun, Z., Hou, W., Ji, F., Lin, X., Tian, H., Zheng, W., Chen, M., Liu, C., Wang, W. & Chen, C. 2019. Surprising anticancer activities of psychiatric medications: old drugs offer new hope for patients with brain cancer, Frontiers in Pharmacology. 10: 1262. https://doi.org/10.3389/fphar.2019.01262
58. Zimmering, S., Cruces, M.P., Pimentel, E., Arceo, C., Carrasco, G. & Olvera, O. 1997. On the recovery of single spots with the flr phenotype in the wing spot test in Drosophila. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 379(1): 77-82. https://doi.org/10.1016/S0027-5107(97)00110-3

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Details

Primary Language	English
Subjects	Toxicology
Journal Section	Research Article/Araştırma Makalesi
Authors	Çağla Ersöz This is me 0000-0001-6535-8714 Deniz Altun Çolak 0000-0002-3576-0355
Project Number	FYL-2018-560
Publication Date	October 15, 2023
Submission Date	May 30, 2023
Acceptance Date	September 18, 2023
Published in Issue	Year 2023 Volume: 24 Issue: 2

Cite

APA	Ersöz, Ç., & Altun Çolak, D. (2023). EFFECT OF BITTER MELON AGAINST CISPLATIN AND VALPROIC ACID-INDUCED GENOTOXICITY IN Drosophila melanogaster Meigen. Trakya University Journal of Natural Sciences, 24(2), 57-66. https://doi.org/10.23902/trkjnat.1302555
AMA	Ersöz Ç, Altun Çolak D. EFFECT OF BITTER MELON AGAINST CISPLATIN AND VALPROIC ACID-INDUCED GENOTOXICITY IN Drosophila melanogaster Meigen. Trakya Univ J Nat Sci. October 2023;24(2):57-66. doi:10.23902/trkjnat.1302555
Chicago	Ersöz, Çağla, and Deniz Altun Çolak. “EFFECT OF BITTER MELON AGAINST CISPLATIN AND VALPROIC ACID-INDUCED GENOTOXICITY IN Drosophila Melanogaster Meigen”. Trakya University Journal of Natural Sciences 24, no. 2 (October 2023): 57-66. https://doi.org/10.23902/trkjnat.1302555.
EndNote	Ersöz Ç, Altun Çolak D (October 1, 2023) EFFECT OF BITTER MELON AGAINST CISPLATIN AND VALPROIC ACID-INDUCED GENOTOXICITY IN Drosophila melanogaster Meigen. Trakya University Journal of Natural Sciences 24 2 57–66.
IEEE	Ç. Ersöz and D. Altun Çolak, “EFFECT OF BITTER MELON AGAINST CISPLATIN AND VALPROIC ACID-INDUCED GENOTOXICITY IN Drosophila melanogaster Meigen”, Trakya Univ J Nat Sci, vol. 24, no. 2, pp. 57–66, 2023, doi: 10.23902/trkjnat.1302555.
ISNAD	Ersöz, Çağla - Altun Çolak, Deniz. “EFFECT OF BITTER MELON AGAINST CISPLATIN AND VALPROIC ACID-INDUCED GENOTOXICITY IN Drosophila Melanogaster Meigen”. Trakya University Journal of Natural Sciences 24/2 (October 2023), 57-66. https://doi.org/10.23902/trkjnat.1302555.
JAMA	Ersöz Ç, Altun Çolak D. EFFECT OF BITTER MELON AGAINST CISPLATIN AND VALPROIC ACID-INDUCED GENOTOXICITY IN Drosophila melanogaster Meigen. Trakya Univ J Nat Sci. 2023;24:57–66.
MLA	Ersöz, Çağla and Deniz Altun Çolak. “EFFECT OF BITTER MELON AGAINST CISPLATIN AND VALPROIC ACID-INDUCED GENOTOXICITY IN Drosophila Melanogaster Meigen”. Trakya University Journal of Natural Sciences, vol. 24, no. 2, 2023, pp. 57-66, doi:10.23902/trkjnat.1302555.
Vancouver	Ersöz Ç, Altun Çolak D. EFFECT OF BITTER MELON AGAINST CISPLATIN AND VALPROIC ACID-INDUCED GENOTOXICITY IN Drosophila melanogaster Meigen. Trakya Univ J Nat Sci. 2023;24(2):57-66.

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