In vitro culture of nodal cuttings in kiwifruit

Year 2023, Volume: 5 Issue: 2, 113 - 122, 30.12.2023

Mehmet Tütüncü Muharrem Özcan

https://doi.org/10.53663/turjfas.1396240

Abstract

This study aimed to determine the effects of plant growth regulators and different medium on in vitro propagation of ˈHaywardˈ and ˈZespri Goldˈ kiwifruits. Micro-cuttings were used as explant sources and cultured in MS and WPM medium containing different concentrations of BA and GA3. Subcultures were carried out at intervals of 4-6 weeks. Contamination rate (%), blackening rate (%), callus development rate (%), shoot rate (%), and propagation coefficient (plantlet/explant) were determined. The micropropagation experiment was carried out according to the randomized plot design. The arc-sin transformation was applied to the percentage values before analysis. Significance levels of the means were compared with the LSD test. It was determined that 0.1% mercury chloride application increased the sterilization efficiency. As the BA concentration in the medium increased, the rate of callus formation of the explants increased. While there was no difference in shoot rates of explants cultured on MS and WPM medium, the propagation rate was higher in MS medium. The propagation coefficient of the ˈHaywardˈ kiwi cultivar was 3.08 in MS medium containing 1.0 mgL-1 BA + 0.5 mgL-1 GA3. MS medium can be recommended as a basic medium for in vitro kiwif propagation.

Keywords

Kiwifruit, Micropropagation, Node culture, Sterilization

References

• Alp, T. (2017). Kiwi. Ordu Ticaret Borsası Yayını, (s 25), 77.
• Barbieri, C., & Morini, S. (1987). Plant regeneration from Actinidia callus cultures. Journal of Horticultural Science, 62(1), 107-109. https://doi.org/10.1080/14620316.1987.11515757
• Cassells, A. C. (1991). Problems in tissue culture: culture contamination. In Micropropagation: technology and application (pp. 31-44). Dordrecht: Springer Netherlands. https://doi.org/10.1007/978-94-009-2075-0_3
• Debenham, M. C., Seelye, J. F., & Mullan, A. C. (2014, August). An in vitro repository for clonal kiwifruit. In XXIX International Horticultural Congress on Horticulture: Sustaining Lives, Livelihoods and Landscapes (IHC2014): 1113 (pp. 93-98). https://doi.org/10.17660/ActaHortic.2016.1113.13
• Ding, S. L., Zhu, X. Z., & Yu, H. M. (1997). Kiwifruit tissue culture. China Fruits, 2, 27-29.
• Gui, Y. L. (1979). Callus induction from stem segments and plantlet regeneration of Chinese gooseberry. Acta Botanica Sinica, 21(4), 339-344.
• Food and Agriculture Organization of The United Nations (2021). Crops and livestock products. https://www.fao.org/faostat/en/#home
• Kim, M., Kim, S. C., Moon, D. Y., & Song, K. J. (2007). Rapid shoot propagation from micro-cross sections of kiwifruit (Actinidia deliciosa cv.‘Hayward’). Journal of Plant Biology, 50, 681-686. https://doi.org/10.1007/BF03030613
• Kumar, S., & Sharma, D. R. (2002). Review Article In vitro propagation of kiwifruit. The Journal of Horticultural Science and Biotechnology, 77(5), 503-508. https://doi.org/10.1080/14620316.2002.11511530
• Kumar, S., C. Subodh, H. Gupta & Sharma, D. R. (1998) Micropropagation of Actinidia deliciosa from axillary buds. Phytomorphology, 48(3), 303-307.
• Leifert, C., Morris, C. E., & Waites, W. M. (1994). Ecology of microbial saprophytes and pathogens in tissue culture and field-grown plants: reasons for contamination problems in vitro. Critical Reviews in Plant Sciences, 13(2), 139-183. https://doi.org/10.1080/07352689409701912.
• Lin, Q. L., Chen, Z. Q., & Wu, J. S. (1994). Propagation in vitro of some excellent clones of kiwifruit. Journal of Fujian Agricultural University, 23(3), 271-274.
• Lloyd, G., & McCown, B. (1980). Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Combined Proceedings, International Plant Propagators' Society, 30, 421-427.
• Marino, G., & Bertazza, G. (1990). Micropropagation of Actinidia deliciosa cvs.‘Hayward’ and ‘Tomuri’. Scientia Horticulturae, 45(1-2), 65-74. https://doi.org/10.1016/0304-4238(90)90069-Q
• Monette, P. L. (1986). Micropropagation of kiwifruit using non-axenic shoot tips. Plant Cell, Tissue and Organ Culture, 6, 73-82. https://doi.org/10.1007/BF00037760
• Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
• Pedroso, M. C., Oliveira, M. M., & Pais, M. S. S. (1992). Micropropagation and simultaneous rooting of Actinidia deliciosa var. deliciosa Hayward'. HortScience, 27(5), 443-445. https://doi.org/10.21273/HORTSCI.27.5.443
• Prado, M. J., Gonzalez, M. V., Romo, S., & Herrera, M. T. (2007). Adventitious plant regeneration on leaf explants from adult male kiwifruit and AFLP analysis of genetic variation. Plant Cell, Tissue and Organ Culture, 88(1), 1-10. https://doi.org/10.1007/s11240-006-9116-0
• Ying-qian, Q., & Dong-ping, Y. (1991, February). Advances in Actinidia research in China. In II International Symposium on Kiwifruit, 297 (pp. 51-56). https://doi.org/10.17660/ActaHortic.1992.297.3
• Rugini, E., & Gutiérrez-Pesce, P. (2003). Micropropagation of kiwifruit (Actinidia spp.). In Micropropagation of Woody trees and fruits (pp. 647-669). Dordrecht: Springer Netherlands. https://doi.org/10.1007/978-94-010-0125-0_22
• Singh, C. R. (2018). Review on problems and its remedy in plant tissue culture. Asian Journal of Biological Sciences, 11(4), 165-172. https://doi.org/10.3923/ajbs.2018
• Sivritepe, N., & Tuğ, Y. (2011). Hayward ve Matua kiwi çeşitlerinde mikro çoğaltım. Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 25(1), 97-108.
• Souad, B., Lionakis, S. M., & Gerasopoulos, D. (1998). Effects of explant type on proliferation and rooting of kiwi in vitro. Advances in Horticultural Science, 12(3), 123-126.
• Tanimoto, G. (1994). Propagation “In: Kiwifruit Growing and Handling”. Univ. of California, Division of Agriculture and Natural Resources, Publication, 3344, 21-24.
• Üçler, A. Ö. Yahyaoğlu, Z. & Güneş, İ. (2000). Sürgün çoğaltılması ile in vıtro’da elde edilen kiwi (Actinidia chinensis Planch.) sürgünlerinin farklı yöntemlerle köklendirilmesi üzerine bir çalışma. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 1 (1): 10-17.
• Wang, D., & Gui, Y. L. (1988). Tissue culture of fruit crops in China. Hortscience, 23(6), 962-965.
• Wang, T., & Gleave, A. P. (2012). Applications of biotechnology in kiwifruit (Actinidia). Innovations in Biotechnology, 1-30. https://doi.org/10.5772/28673
• Wiyaporn S., S. Subhadrabandhu & Sahavacharin, O. (1990). In vitro vegetative multiplication of kiwi plant. Acta Horticulturae, 279, 447-459. https://doi.org/10.17660/ActaHortic.1990.279.4