Cytotoxic Effect of Clethodim Exposure on Liver Cells&

Year 2024, Volume: 11 Issue: 2, 454 - 462, 30.04.2024

Ergün Ergenekon Ömer Erman Nihal Şimşek Özek

https://doi.org/10.30910/turkjans.1442321

Abstract

Clethodim, a herbicide group pesticide, is classified as a toxicity class II agent. The development toxicity, immunotoxicity, neurotoxicity and reproductive toxicity of this herbicide have been demonstrated in zebrafish and rat. There are no in vitro studies on the hepatoxic effects of clethodim. In this study, for the first time, clethodim was tested with liver cell line models. THLE-2 and B129 cells were treated with a wide range of clethodim for 96 hours. After that, its hepatoxic effects were evaluated by MTT and SRB analyses. Both assays revealed that the cellular proliferation was strikingly decreased with the dose dependent manner. Especially, 1000 µg/ml dose of the clethodim exposure supressed the cellular proliferation at a %90 and %75 for B129 and THLE-2 cells. Moreover, a significant reduction was acquired in both cells number and volume with the high doses of this cyclohexanedione class herbicide. These cytotoxic impacts can be strongly related to the herbicide elaved oxidative stress. In the light of our results, the long and high doses of clethodim exposure has a hepatoxic effect and its toxic target organ is be liver. Hence, further studies on the use of clethodim-based herbicides are urgently needed.

Keywords

Clethodim, human liver cell, mouse liver cell, MTT, SRB assay.

Supporting Institution

Atatürk University Scientific Research Projects Coordination Unit

Project Number

BAP, No: FYL-2023-12045

Thanks

We would be very grateful to is Anatolia High Technology Application and Research Center (DAYTAM) of Atatürk University for supporting lab facility.

Klethodim’e Maruz Kalmanın Karaciğer Hücreleri Üzerindeki Sitotoksik Etkisi&

Abstract

Herbisit grubu pestisit olan kletodim, toksisite sınıfı II ajan olarak sınıflandırılır. Bu herbisitin gelişim toksisitesi, immünotoksisitesi, nörotoksisitesi ve üreme toksisitesi zebra balığı ve sıçanlarda gösterilmiştir. Kletodim'in hepatoksik etkilerine ilişkin in vitro çalışma bulunmamaktadır. Bu çalışmada ilk kez kletodim karaciğer hücre hattı modelleriyle test edildi. THLE-2 ve B129 hücreleri, 96 saat boyunca geniş bir aralıktaki klethodim ile muamele edildi. Daha sonra hepatoksik etkileri MTT ve SRB testleri ile değerlendirildi. Her iki analiz de hücresel proliferasyonun doza bağlı olarak çarpıcı biçimde azaldığını ortaya çıkardı. Özellikle 1000 µg/ml dozunda kletodim maruziyetinin B129 ve THLE-2 hücrelerinde hücresel poliferasyonu %90 ve %75 oranında baskıladığı görüldü. Ayrıca sikloheksandion sınıfı herbisitin yüksek dozları ile hem hücre sayısında hem de hacminde önemli bir azalma elde edilmiştir. Bu sitotoksik etkiler, herbisitlerin neden olduğu oksidatif stresle güçlü bir şekilde ilişkili olabilir. Sonuçlarımız ışığında, kletodim'e uzun ve yüksek dozda maruz kalmanın hepatoksik etkiye sahip olduğu ve toksik hedef organının karaciğer olduğu görülmüştür. Bu nedenle, kletodim bazlı herbisit kullanımı hakkınca acil olarak daha fazla çalışmaya ihtiyaç vardır.

Keywords

Kletodim, insan karaciğer hücresi, fare karaciğer hücresi, MTT, SRB testleri.

Supporting Institution

Atatürk Üniversitesi Bilimsel Araştırmalar ve Koordinasyon Birimi

Project Number

BAP, No: FYL-2023-12045

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