Antibacterial Activity of Verbascum antinori

Yıl 2018, Cilt: 10 Sayı: 3, 395 - 398, 23.10.2018

Basaran Dulger Gorkem Dulger

https://doi.org/10.18521/ktd.378960

Cited By: 1

Öz

Objective: In current study, biological screening of the methanol extract obtained from Verbascum antinori Boiss. et Heldr. (Scrophulariaceae) was carry out on antibacterial effects.

Methods: Antibacterial activity of the extract was evaluated for Staphylococcus aureus ATCC 6538P, Proteus vulgaris ATCC 8427, Klebsiella pneumoniae UC57, Micrococcus luteus CCM 169, Escherichia coli ATCC 11230, Listeria monocytogenes ATCC 15313, Bacillus cereus ATCC 7064 and Pseudomonas aeruginosa ATCC 27853 by disk diffusion method.

Results: The extract has potential effect against the Gram-positive bacteria, but no activity was observed against the Gram-negative bacteria used in the present study.

Conclusions: On the basis of the mentioned results, V. antinori may be a potential source of antibacterial agent.

Anahtar Kelimeler

Antibacterial activity, Verbascum antinori

Verbascum antinori Bitkisinin Antibakteriyal Aktivitesi

Öz

Amaç: Bu çalışmada, Verbascum antinori Boiss. et Heldr. (Scrophulariaceae) bitkisinden elde edilen metanol ekstraktının antibakteriyal aktivitesi araştırılmıştır.

Gereç ve Yöntem: Ekstraktın antibakteriyal aktivitesi, Staphylococcus aureus ATCC 6538P, Proteus vulgaris ATCC 8427, Klebsiella pneumoniae UC57, Micrococcus luteus CCM 169, Escherichia coli ATCC 11230, Listeria monocytogenes ATCC 15313, Bacillus cereus ATCC 7064 ve Pseudomonas aeruginosa ATCC 27853 bakterilerine karşı disk difüzyon metodu kullanılarak belirlenmiştir.

Bulgular: Elde edilen ekstraktın, çalışmada kullanılan Gram-pozitif bakterilere karşı oldukça etkin olduğu, ancak Gram-negatif bakterilere karşı ise etkisiz olduğu saptanmıştır.

Sonuç: V. antinori bitkisinden elde edilen ekstraktın antibakteriyal ajan olarak kullanılabilirliği saptanmış ve ilerki çalışmalarda bu bitkinin antibakteriyal bileşiklerinin belirlenmesine yönelik çalışmaların gerekliliği öngörülmüştür.

Anahtar Kelimeler

Antibakteriyal Aktivite, Verbascum antinori

Kaynakça

• 1. Akdemir Z, Kahraman C, Tatli I, Kupeli Akkol E, Suntar I, Keles H. Bioassay-guided isolation of anti-inflammatory, antinociceptive and wound healer glycosides from the flowers of Verbascum mucronatum Lam. J Ethnopharmacol 2011; 136:436–43. 2. Firat M. Verbascum kurdistanicum (Scrophulariaceae), a new species from Hakkâri, Turkey. PhytoKeys 2015; 52: 89–94. 3. Zeybek N. Pharmaceutical Botany (in Turkish). E.Ü. Eczacılık Fak. Yayınları, No. 1. Bornova Izmir, E.Ü. Basımevi, 1985. 4. Khan NH, nur-E Kamal M.S.A, Rahman M. Antibacterial activity of Euphorbia thymifolia Linn. Indian J Med Res 1988; 87:395-397. 5. Collins CM, Lyne PM. Microbiological Methods. Butterworths and Co. Ltd., London, 1987; p. 316. 6. Dulger B. Antimicrobial activity of some endemic Scrophulariaceae from Turkey. Pharm Biol 2006; 44: 672-676. 7. Klimek B, Lavaud C, Massiot G. Saponins from Verbascum nigru. Phytochemistry 1992; 31: 4368-4370 8. Nazemiyeh H, Rahman MM, Gibbons S, Nahar L, De-Lazar A, Ghahramani MA, Talebpour AH, Sarker SD. Assessment of the antibacterial activity of phenylethanoid glycosides from Phlomis lanceolata against multiple-drug-resistant strains of Staphylococcus aureus. J Nat Med 2008; 62: 91-95. 9. Tatli II, Akdemir ZS. Chemical constitituents of Verbascum L. species. FABAD J Pharm Sci 2004; 29, 93-107. 10. Tatli II, Schuhly W, Akdemir ZS. Secondary metabolites from bioactive methanolic extract of Verbascum pysnostachyum Boiss. & Helder flowers. Hacettepe University J Fac Pharm 2007; 27:23-32. 11. Ozcan B, Esen M, Caliskan M, Mothana RA, Cihan AC, Yolcu H. Antimicrobial and antioxidant activities of the various extracts of Verbascum pinetorum Boiss. O. Kuntze (Scrophulariaceae). Eur Rev Med Pharmacol Sci 2011; 15: 900-905 12. Projen SJ, Novick RP. The molecular basis of pathogenicity. In: Archer G, Crossley K, Editors. The Stapyhylocci in Human Diseases. New York: Churcill Livingstone 1997; p. 55-87. 13. National Nosocomial Infections Survellianca (NNIS) System Report, data summary from January 1992 through June 2004, isuused October 2004. Am J Infect Control 2004; 32:470-485. 14. Dulger B, Kırmızı S, Arslan H, Guleryuz G. Antimicrobial Activity of Three Endemic Verbascum L. Species. Pharm Biol 2002; 40(8):587-589. 15. Dulger B, Ugurlu E, Aki C, Suerdem TB, Camdeviren A, Tazeler G. Evaluation of antimicrobial activity of some endemic Verbascum, Sideritis, and Stachys species from Turkey. Pharm Biol 2005; 43(3), 270-274. 16. Dulger B, Gonuz A. Antimicrobial Acitivity of The Some Endemic Verbascum, Salvia, and Stachys species. Pharm Biol 2004; 42(4-5):301-304. 17. Dulger B, Ugurlu E. Evaluation of antimicrobial activity of some endemic Scrophulariaceae members from Turkey. Pharm Biol 2005; 43(3):275-279. 18. Klimek B. Verbascoside in the flowers of some Verbascum species. Acta Pol Pharm 1991; 48 (3-4):51-54. 19. Kalpoutzakis E, Aligiannis N, Mitakou S, Skaltsounis AL. Verbaspinoside, a new iridoid glycoside from Verbascum spinosum. J Nat Prod 1999; 62: 342-344. 20. Hartleb I, Seifert K. Triterpenoid saponins from Verbascum songaricum. Phytochemistry 1995; 38: 221-224. 21. Farbood MI, MacNeil JH, Ostovar K. Effects of rosemary spice extractive on growth of microorganisms in meats. J Milk Food Technol 1976; 39:675-679. 22. Cetin TE, Gurler N. Bakterilerin antibiyotiklere duyarlilik deneyinin yapilmasi. Kukem Dergisi 1989; 12: 2-5. 23. Outtara B, Simard RE, Holley RA, Pietta GJP, Begin A. Antimicrobial activity of selected fatty acids and essential oils against six meat spoilage organisms. Int J Food Microbiol 1997; 37: 155-162.