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Potential Importance of miRNA Studies in Identification of Body Fluids: A Current Perspective

Yıl 2024, Cilt: 38 Sayı: 1, 80 - 92, 29.04.2024

Öz

Although there are many findings at crime scenes that can be considered as evidence, biological samples are among the most important types of findings that reveal the relationship between the victim, the suspect and the crime scene; blood, menstrual blood, semen, vaginal secretions and saliva. Enzymatic, serological and microscopic methods have been used for years in the identification of biological fluids used in identification. Since biological samples are found at crime scenes in very small quantities and are often in a degraded state, identification of the samples becomes difficult. However, recently, forensic studies on the identification of body fluids with miRNAs, which are accepted as "non degraded evidence" due to their high stability, have reversed this situation. The general opinion in the majority of studies is that miRNAs can be used in the detection of body fluids. The modeling used during analysis is of great importance, especially in the correct analysis and classification of mixtures and risky samples due to their high sensitivity. As a result, according to the literature review, it has been suggested that the reason for not reaching definitive and common results in miRNA studies is the diversity or difference of analysis and modeling methods used in the studies. For future studies, it is recommended to conduct further research with a general and accepted common method. In this review, it is aimed to summarize various studies conducted recently on miRNAs from different aspects.

Kaynakça

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  • Hanson EK, Lubenow H, Ballantyne J. Identification of forensically relevant body fluids using a panel of differentially expressed microRNAs. Anal Biochem. 2009;387:303–14. https://doi.org/10.1016/j.ab.2009.01.037
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Vücut Sıvılarının Kimliklendirilmesinde miRNA Çalışmalarının Potansiyel Önemi: Güncel Bir Bakış

Yıl 2024, Cilt: 38 Sayı: 1, 80 - 92, 29.04.2024

Öz

Olay yerlerinde, delil niteliği kazanabilecek olan birçok bulgu bulunsa da mağdur, şüpheli ve olay yeri ilişkisini ortaya çıkaran en önemli bulgu türlerinden olan biyolojik örnekler; kan, menstrual kan, meni, vajinal salgılar ve tükürüktür. Kimliklendirmede kullanılan biyolojik sıvıların tanımlanmasında enzimatik, serolojik ve mikroskobik yöntemler yıllardır kullanılmaktadır. Biyolojik örnekler olay yerlerinde çok küçük miktarlarda bulunduklarından ve çoğunlukla bozunmuş halde olduklarından dolayı örneklerin tespiti zorlaşmaktadır. Ancak son zamanlarda, yüksek stabiliteleri nedeniyle ‘’bozunmadan kalabilecek delil’’ niteliğinde kabul gören miRNA’lar ile vücut sıvılarının tanımlamasına yönelik yapılan adli çalışmalar bu durumu tersine çevirmektedir. Yapılan çalışmaların çoğunluğundaki genel görüş, miRNA’ların vücut sıvılarının tespitinde kullanılabilir olduğu yönündedir. Özellikle yüksek hassasiyetleri nedeniyle karışım halinde bulunan ve riskli örneklerin doğru bir şekilde analiz edilip sınıflandırılmasında, analiz yapılırken kullanılan modellemeler büyük önem taşımaktadır. Sonuç olarak yapılan literatür taramalarına göre, miRNA çalışmalarında kesin ve ortak sonuçlara tam anlamıyla ulaşılamamış olmanın nedeni olarak, çalışmalarda kullanılan analiz ve modelleme yöntemlerinin çeşitliliği veya farklılığı öne sürülmüştür. İleride yapılacak olan çalışmalar için genel ve kabul görmüş ortak bir yöntemle daha fazla araştırma yapılması önerilmektedir. Bu derlemede, son zamanlarda miRNA’lar ile yapılan çeşitli çalışmaların farklı yönleriyle ele alınarak özetlenmesi amaçlanmıştır.

Kaynakça

  • Çetli E, Tatar D, Özkoçak V. Adli bilimlerde DNA parmak izine adli genetik ve adli antropolojik bakış. Bitlis Eren Üniversitesi Fen Bilimleri Derg. 2019;8(4):1545–56. https://doi.org/10.17798/bitlisfen.537780
  • Al-Mawlah YH, Jebor MA, Abdulla AA. The effect of mixing seminal fluid and vaginal secretion on the expression of miRNA markers in a simulated forensic scientific detection. Ann Rom Soc Cell Biol. 2021;25(4):11477–82.
  • Alakoç YD. Adli bilimlerde DNA analizleri. Ankara Sağlık Hizmetleri Derg. 2019;9(2):1–8.
  • He H, Han N, Ji C, Zhao Y, Hu S, Kong Q, Ye J, Ji A, Sun Q. Identification of five types of forensic body fluids based on stepwise discriminant analysis. Forensic Sci Int Genet. 2020;48:102337. https://doi.org/10.1016/j. fsigen.2020.102337
  • Rao A, Rana M, Pradhan A, Sinha M. RNA and DNA based identification of body fluids. Forensic DNA Typing: Principles, Applications and Advancements. 2020;87–104. https://doi.org/10.1007/978-981-15-6655-4_5
  • Rhodes C, Lewis C, Szekely J, Campbell A, Creighton MRA, Boone E, Seashols-Williams S. Developmental validation of a microRNA panel using quadratic discriminant analysis for the classification of seven forensically relevant body fluids. Forensic Sci Int Genet. 2022;102692. https://doi.org/10.1016/j.fsigen.2022.102692
  • Glynn CL. Potential applications of microRNA profiling to forensic investigations. RNA. 2020;26(1):1–9. https://doi. org/10.1261/rna.072173.119
  • Li Z, Lv M, Peng D, Xiao X, Fang Z, Wang Q, Zhang L. Feasibility of using probabilistic methods to analyse microRNA quantitative data in forensically relevant body fluids: a proofof-principle study. Int J Legal Med. 2021;135:2247–61. https://doi.org/10.1007/s00414-021-02678-w
  • Hanson EK, Lubenow H, Ballantyne J. Identification of forensically relevant body fluids using a panel of differentially expressed microRNAs. Anal Biochem. 2009;387:303–14. https://doi.org/10.1016/j.ab.2009.01.037
  • Han J, Han N, Xu Z, Zhang C, Liu J, Ruan M. Expression profile of circular RNA and construction of circular RNA, Micro RNA network in salivary adenoid cystic carcinoma. Cancer Cell Int. 2021;21(1):1–11. https://doi.org/10.1186/ s12935-020-01681-2
  • Menini M, De Giovanni E, Bagnasco F, Delucchi F, Pera F, Baldi D, Pesce P. Salivary micro-RNA and oral squamous cell carcinoma: a systematic review. J Pers Med. 2021;11(2):101. https://doi.org/10.3390/jpm11020101
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  • Lewis CA, Layne TR, Seashols‐Williams SJ. Detection of microRNAs in DNA extractions for forensic biological source identification. J Forensic Sci. 2019;64(6):1823–30. https://doi.org/10.1111/1556-4029.14070
  • Praihirunkit P. miRNAs: perspective towards the use for body fluid identification. Siriraj Med J. 2020;72(6):512–26. https://doi.org/10.33192/Smj.2020.70
  • Xiao Y, Chen D, Peng D, Li Z, Qu S, Zhang R, Liu G, Zheng Y, Mengyu T, Xue J, Zhang Y, Zhu J, Liang W. Establishment of a co-analysis system for personal identification and body fluid identification: a preliminary report. Int J Legal Med. 2022;136(6):1565–75. https://doi.org/10.1007/s00414-022- 02886-y
  • Turiello R, Nouwairi RL, Landers JP. Taking the microfluidic approach to nucleic acid analysis in forensics: review and perspectives. Forensic Sci Int Genet. 2023;63:102824. https://doi.org/10.1016/j.fsigen.2022.102824
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  • Bamberg M, Bruder M, Dierig L, Kunz SN, Schwender M, Wiegand P. Best of both: A simultaneous analysis of mRNA and miRNA markers for body fluid identification. Forensic Sci Int Genet. 2022;59:102707. https://doi.org/10.1016/j.fsigen.2022.102707
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  • Lewis CA, Seashols‐Williams SJ. A combined molecular approach utilizing microbial DNA and microRNAs in a qPCR multiplex for the classification of five forensically relevant body fluids. J Forensic Sci. 2024;69:282–90. https://doi.org/10.1111/1556-4029.15400
Toplam 76 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Adli Biyoloji
Bölüm Derlemeler
Yazarlar

Özlem Arat 0000-0002-4685-5990

Dilek Kaya-akyüzlü 0000-0002-3305-0587

Yayımlanma Tarihi 29 Nisan 2024
Gönderilme Tarihi 27 Şubat 2024
Kabul Tarihi 15 Nisan 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 38 Sayı: 1

Kaynak Göster

Vancouver Arat Ö, Kaya-akyüzlü D. Vücut Sıvılarının Kimliklendirilmesinde miRNA Çalışmalarının Potansiyel Önemi: Güncel Bir Bakış. ATD. 2024;38(1):80-92.

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