Lycopodium clavatum ekstraktının SKBR-3 insan meme kanseri hücreleri üzerindeki apoptotik etkileri

Yıl 2023, Cilt: 48 Sayı: 4, 1207 - 1216, 29.12.2023

Mohammad Reza Dastouri Yusuf Küçükbağrıaçık

https://doi.org/10.17826/cumj.1336606

Öz

Amaç: Meme kanseri dünya çapında önemli bir halk sağlığı sorunudur. Bitkisel kaynaklı doğal bileşikler, düşük toksisiteleri, güvenilirlikleri ve potansiyel etkinlikleri nedeniyle kanserle mücadelede umut verici adaylar olarak kabul edilmektedir. Bu çalışmada, Lycopodium clavatum'un etanol ekstraktının SKBR-3 insan meme kanseri hücreleri üzerindeki apoptotik etkisi araştırılmıştır.
Gereç ve Yöntem: İnsan meme kanseri hücrelerinin canlılığını değerlendirmek için farklı dozlarda (100, 200 ve 300 µg/mL) ve sürelerde (12, 24 ve 48 saat) Lycopodium clavatum etanol ekstraktı uygulamasının etkisi, WST-1 sitotoksisite testi kullanılarak incelenmiştir. Ayrıca, Lycopodium clavatum etanol ekstraktının apoptoz mekanizması, içsel (BAX ve Kaspaz-9) ve dışsal (Kaspaz-8 ve Kaspaz-3) yolaklar ile araştırılmıştır.
Bulgular: Lycopodium clavatum etanol ekstraktı uygulaması SKBR-3 hücrelerinde bu etkinin uygulanan doz ve tedavi süresine bağlı olarak sitotoksik bir etki göstermiştir. LC-EE ile 12 saatlik inkübasyondan sonra kontrol grubuna kıyasla; 100 µg/mL verilen grupta %10, 200 µg/mL verilen grupta %25, 300 µg/mL verilen grupta %40’lık bir hücre ölümü gözlenmiştir. Ayrıca, yapılan gözlemler Lycopodium clavatum tedavisinin BAX, Kaspaz-9, Kaspaz-8 ve Kaspaz-3 gibi apoptozla ilişkili proteinlerin aktivasyonunu tetiklediğini göstermektedir.
Sonuç: Lycopodium clavatum etanol ekstraktının SKBR-3 hücrelerinde anti-kanser etkisini içsel ve dışsal apoptotik yolları aktive ederek gerçekleştirebileceğini ortaya koymuştur. Bu bulgular, Lycopodium clavatum'un insan meme kanserine karşı etkili bir anti-kanser ajanı olarak yeni terapötik stratejilerin geliştirilmesine yardımcı olabileceğini göstermektedir.

Anahtar Kelimeler

Lycopodium clavatum ekstraktı, SKBR-3, insan meme kanseri, sitotoksisite, apoptoz

Proje Numarası

1919B012217673

Apoptotic effects of Lycopodium clavatum extract on SKBR-3 human breast cancer cells

Öz

Purpose: Breast cancer is an important public health problem worldwide. Natural compounds derived from plants have emerged as promising candidates for fighting cancer due to their safety, minimal toxicity, and potential effectiveness. This study investigated the apoptotic effect of the ethanol extract of Lycopodium clavatum on SKBR-3 human breast cancer cells.
Materials and Methods: The effect of applying Lycopodium clavatum ethanol extract at different doses (100, 200, and 300 µg/mL) and duration (12, 24, and 48 hours) to evaluate the viability of human breast cancer cells was investigated using the WST-1 cytotoxicity test. Also, the mechanism of apoptosis of Lycopodium clavatum ethanol extract was investigated by intrinsic (BAX and Caspase-9) and extrinsic (Caspase-8 and Caspase-3) pathways.
Results: The application of Lycopodium clavatum ethanol extract had a cytotoxic effect on SKBR-3 cells and this effect was dependent on the dose and duration of treatment. After 12 hours of incubation with LC-EE, 10%, 25%, and 40% cell death were observed in the 100, 200, and 300 µg/mL groups, respectively, compared to the control group. Additionally, our findings demonstrate that Lycopodium clavatum treatment induces the stimulation of apoptotic proteins, including BAX, Caspase-9, Caspase-8, and Caspase-3.
Conclusion: The anti-cancer effect of Lycopodium clavatum ethanol extract in SKBR-3 cells was determined by activating intrinsic and extrinsic apoptotic pathways. These findings suggest that Lycopodium clavatum may assist in the development of new therapeutic strategies as an effective anti-cancer agent against human breast cancer.

Anahtar Kelimeler

Lycopodium clavatum extract, SKBR-3, human breast cancer, cytotoxicity, apoptosis

Destekleyen Kurum

Scientific and Technological Research Council of Turkey (TUBITAK)

Proje Numarası

1919B012217673

Kaynakça

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