Kurkumin ve beta-karotenin sisplatinin neden olduğu over hasarı üzerindeki etkilerinin karşılaştırılması

Yıl 2023, Cilt: 48 Sayı: 4, 1248 - 1257, 29.12.2023

Ayşe Ceyhan Münevver Baran Pınar Alişan Suna Özge Cengiz Mat Arzu Yay

https://doi.org/10.17826/cumj.1345688

Öz

Amaç: Cisplatin (CIS) kaynaklı over hasarına karşı kurkumin (CUR) ve beta-karotenin (BC) potansiyel koruyucu etkilerinin histolojik ve immünohistokimyasal yöntemlerle araştırılması amaçlanmıştır.
Gereç ve Yöntem: Çalışmada, rastgele yedi gruba ayrılan 56 dişi Wistar albino sıçan kullanıldı. Kontrol sıçanları herhangi bir tedavi görmedi; sham grubuna gavajla 1 ml/kg susam yağı verildi; CIS grubuna 5 mg/kg CIS; CUR grubuna 200 mg/kg CUR; BC grubuna 200 mg/kg BC; CUR + CIS grubuna, CUR uygulamasından sonra CIS uygulandı; BC + CIS grubu, BC ile ön işleme tabi tutuldu, ardından CIS uygulandı. Tüm grupların overleri son uygulamadan beş gün sonra eksize edildi. Elde edilen dokularda, histopatolojik değerlendirme yapıldı ve folikül rezervi hesaplandı. Anti-Mullerian hormon (AMH) ve nükleer faktör-kappa B (NF-κB) ekspresyonu immünohistokimyasal olarak saptandı. Apoptoz ise, TUNEL yöntemi kullanılarak değerlendirildi.
Bulgular: CUR ve BC'nin, CIS'in neden olduğu azalan primordial, primer, preantral, sekonder ve tersiyer folikül sayılarına karşı koruyucu olduğu gözlendi. AMH immünoreaktivitesi, kontrol ve CUR + CIS grupları için hemen hemen aynıydı. CUR ve BC gruplarında kontrollere kıyasla over NF-κB ekspresyon seviyelerini düşürdü. TUNEL pozitif apoptotik hücre sayıları, CUR'nin BC'den daha güçlü bir anti-apoptotik etki gösterdiğini gösterdi.
Sonuç: CIS’nin neden olduğu over hasarına karşı CUR'nin BC'den daha fazla antioksidan ve antiinflamatuar etkiye sahip olduğunu gösterilmiştir.

Anahtar Kelimeler

Anti-Mullerian hormone, apoptosis, beta-karoten, cisplatin, kurkumin, NF-κB

Proje Numarası

project no. TYL-2016-7069

Comparison of effects of curcumin and beta-carotene on ovarian damage caused by cisplatin

Öz

Purpose: We aimed the potential protective effects of curcumin (CUR) and beta-carotene (BC) against cisplatin (CIS)-induced ovarian damage using histological and immunohistochemical methods.
Materials and Methods: We used 56 female Wistar albino rats, divided randomly into seven groups. Control rats did not receive any treatment; the sham group was administered 1 ml/kg sesame oil by gavage; the CIS group 5 mg/kg CIS; the CUR group 200 mg/kg CUR; the BC group 100 mg/kg BC; the CUR + CIS group, CIS after administration of CUR; the BC + CIS group was pretreated with BC, then administered CIS. The ovaries of all groups were excised five days after the last application. We assessed histopathology and counted and classified follicles. Expression of anti-Mullerian hormone (AMH) and nuclear factor-kappa B (NF-κB) was detected immunohistochemically. Apoptosis was evaluated using the TUNEL method.
Results: CUR and BC are protective against decreased numbers of primordial, primary, preantral, secondary, and tertiary follicles caused by CIS. Both Cur and BC reduced ovarian NF-κB levels in comparison to the control group, and AMH immunoreactivity was almost identical for the control and CUR + CIS groups. Apoptotic cell counts indicated that CUR exerts a stronger anti-apoptotic effect than BC.
Conclusion: CUR has a protective effect against ovarian damage brought on by CIS and greater antioxidant and anti-inflammatory properties than BC.

Anahtar Kelimeler

Anti-Mullerian hormon, Apoptosis, beta-karoten, cisplatin, kurkumin, NF-κB

Destekleyen Kurum

This study was supported by grants from the Erciyes University Scientific Research Projects Unit

Proje Numarası

project no. TYL-2016-7069

Kaynakça

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