Effects of sleeve gastrectomy on N6-methyladenosine modification in duodenal tissue

Yıl 2023, Cilt: 48 Sayı: 4, 1365 - 1373, 29.12.2023

Badel Arslan Hakan Seyit Murat Eser Akyürek Serdal Arslan Damlanur Sakız Çağatay Han Türkseven

https://doi.org/10.17826/cumj.1370487

Öz

Purpose. In this study, the expression levels of METTL3, METTL14; and WTAP genes involved in the N6-methyladenosine (m6A) pathway, regulating cellular processes including cell renewal, differentiation, invasion; and apoptosis, were investigated in duodenal tissue after sleeve gastrectomy (SG).
Material and Methods: Duodenal biopsies were obtained from 24 obese patients before (BG) and 6 months after (AG) sleeve gastrectomy. Pathological evaluation was performed by H&E staining. Expression levels of METTL14, METTL3, and WTAP genes were analysed by Real-Time PCR.
Results: The METTL14 gene was significantly increased after surgery (BG-AG Median (Q1-Q3): 0.36 [0.07-1.52]-9.83 [0.20-68.12]). METTL3 (BG-AG Median (Q1-Q3): 0.49 [0.32-0.84]-0.67 [0.36-20.5]) and WTAP (BG-AG Median (Q1-Q3): 0.95 [0.17-7.65]-5.54 [2.34-66.07]) gene expressions were not altered. However, a strong positive (r=0.692) and significant relationship was found between METTL3 and WTAP. Chronic inflammation 81.32 %, intestinal metaplasia 10.54 %, activity 68.88 %, atrophy 5.32 %. No malignant findings were detected.
Conclusion: For the first time, we demonstrated that sleeve gastrectomy may increase the expression of the METTL14 gene involved in the m6A-methyladenosine pathway in duodenum. This alteration may lead to gastrointestinal tract diseases.

Anahtar Kelimeler

Sleeve Gastrectomy, Bariatric Surgery, Epigenetic, Duodenum, N6-Methyladenosine

Destekleyen Kurum

This study was supported by the authors.

Duodenal dokuda N6-metiladenozin modifikasyonu üzerine sleeve gastrektomi etkileri

Öz

Amaç: Bu retrospektif çalışmada açık kalp cerrahisi geçiren 80 yaş üstü hastaların operasyon öncesi albümin değeri kullanılarak hesaplanan geriatrik nutrisyonel risk indeksinin, ameliyat sonrası mortalite oranını tahmin edebilme gücü ortaya konulmaya çalışıldı.
Gereç ve Yöntem: Temmuz 2019 – Eylül 2022 tarihleri arasında koroner bypass ve kalp kapağı değişimi nedeniyle açık elektif kalp cerrahisi uygulanan toplam 22 hasta (13 erkek, 9 kadın; ortalama yaş: 82,1±2,4 yıl; dağılım, 80-88 yıl) geriatrik nutrisyonel risk indeksi ile retrospektif olarak incelendi. Bu indekse göre iki grup oluşturuldu. 98 ve altındaki değerler riskli, 99 ve üstündeki değerler risksiz grup olarak belirlendi. Riskli grupta 6 hasta vardı. Diğer 16 hasta risksiz grupta bulunuyordu. Tüm hasta grubu cerrahi işlemler, cinsiyet, yaş, vücut kütle indeksi (kg/m2), atrial fibrilasyon, diyabet, hipertansiyon, sigara içiciliği ve operatif özellikler yönüyle karşılaştırıldı.
Bulgular: Çalışmada geriatrik nutrisyonel risk indeksine göre 6 riskli (%27,3) ve 16 risksiz (%72,7) hasta incelendi ve risk varlığı tespit edilen hastalarda mortalite oranı yüksek (%66,7) bulundu. Risk grubundaki hastalar, risk grubu olmayanlara kıyasla daha düşük vücut kitle indeksi ve albümin değerlerine sahipti (sırasıyla 25,0±1,8 ve 30,1±2,4; 28,1±2,4 ve 36,7±3,1). Ayrıca, ortalama entübasyon değerlerinin yüksek bulunduğu belirlendi (11,2±4,2). Diğer parametreler ile risk varlığı arasında anlamlı bir farklılığa rastlanılmadı.
Sonuç: Açık kalp cerrahisi öncesi değerlendirilen geriatrik nutrisyonel risk indeksi, operasyon sonrası mortalite oranını öngörmede güçlü bir belirleyici olarak kullanılabilir.

Anahtar Kelimeler

Geriatrik nutrisyonel risk indeksi, oktogenarian, albümin, malnütrisyon

Kaynakça

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