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Kadmiyum Toksisitesi Altındaki Buğdayda Salisilik Asidin Apoplastik Antioksidatif Sistem Üzerine Etkileri

Yıl 2023, Cilt: 6 Sayı: 1, 46 - 56, 17.12.2023
https://doi.org/10.57244/dfbd.1298625

Öz

Bu çalışmada Salisilik asit (SA) uygulamasının, kadmiyum (Cd) stresine maruz bırakılmış buğdayın (Triticum aestivum L.) Bolal çeşidine ait yapraklarındaki apoplastik antioksidan enzim (katalaz, peroksidaz ve süperoksit dismutaz) aktiviteleri ile lipid peroksidasyonu ve hidrojen peroksit miktarı üzerine etkileri araştırılmıştır. Bitkiler 22/20 oC’de toplam 18 gün büyütülmüşlerdir. Bitki yapraklarına 12. gün farklı konsantrasyonlarda (0.01, 0.1, 1 mM) SA uygulanmış ve bundan 3 gün sonra, bitkilerin yetiştiği ortama farklı konsantrasyonlarda (25, 50, 100 μM) Cd uygulanmıştır. On sekizinci gün bitki yaprakları araştırmamızda deney materyali olarak kullanılmışlardır. SA uygulamaları, 50 ve 100 μM Cd’de katalaz (CAT) aktivitesini artırırken POX aktivitesini düşürmüştür. 25 ve 50 μM Cd’e maruz kalmış buğday yapraklarında ise SOD aktivitesini genelde artırmıştır. Ancak, 100 μM Cd’de aktiviteyi düşürmüştür. SA uygulamaları, 25, 50 ve 100 μM Cd etkisi altındaki buğdayda lipid peroksidasyonunu (LPO) düşürürken 25 μM Cd’de SA’nın 3 konsantrasyonuda H2O2 miktarını artırmıştır. SA 50 μM Cd’de H2O2 miktarını artırmıştır. 100 μM Cd’de 0.01 ve 0.1 mM SA uygulamaları aktiviteyi artırırken, 1 mM ise düşürmüştür.
Sonuçlarımız bitkilere Cd stresine maruz kalmadan uygulanan SA’nın hem apoplastik antiokdidatif enzimleri hem de LPO ve H2O2 miktarlarını düzenleyerek bir koruma sağlayabildiğini göstermektedir.

Kaynakça

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Toplam 35 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Makaleler
Yazarlar

Deniz Tiryaki

Ökkeş Atıcı 0000-0001-6256-5101

Sinem Karakuş 0000-0002-6698-153X

Yayımlanma Tarihi 17 Aralık 2023
Gönderilme Tarihi 17 Mayıs 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 6 Sayı: 1

Kaynak Göster

APA Tiryaki, D., Atıcı, Ö., & Karakuş, S. (2023). Kadmiyum Toksisitesi Altındaki Buğdayda Salisilik Asidin Apoplastik Antioksidatif Sistem Üzerine Etkileri. Doğu Fen Bilimleri Dergisi, 6(1), 46-56. https://doi.org/10.57244/dfbd.1298625