Chemical Composition and Antibacterial Activity of Volatile Compounds Genista carinalis Plant

Yıl 2024, Cilt: 12 Sayı: 2, 1192 - 1200, 29.04.2024

Hilmican Çalışkan Merve Argon Muazzez Gürgan Eser Temine Şabudak

https://doi.org/10.29130/dubited.1316704

Öz

Essential oils play a significant role in the plant chemistry. Natural products have been widely used in antiviral, antibacterial, antiparasitic, antifungal, insecticidal, medicinal and cosmetic applications since the Middle Age. Our aim in this study was to investigate the volatile components of the Genista carinalis Griseb. (Fabaceae) plant grown in the Trakya region by GC-MS method and to determine the antibacterial activity of the raw extracts. Volatile components of n-hexane extract from the plant G. carinalis were investigated using the GC-MS method. As a result of our research on the volatile components of G. carinalis, fifty-two bioactive components were identified. The ethyl acetate extract of G. carinalis was the most effective on both Gram-positive and Gram-negative bacteria tested, with higher antibacterial activity against Gram positive bacteria.

Anahtar Kelimeler

Genista, Genista carinalis, Fabaceae, Essential Oil, Antibacterial activity

Genista carinalis Bitkisinin Uçucu Bileşiklerinin Kimyasal Bileşenleri ve Antibakteriyel Aktivitesi

Öz

Uçucu yağlar, bitki kimyasında önemli bir rol oynamaktadır. Doğal ürünler, Orta Çağ'dan beri antiviral, antibakteriyel, antiparazitik, antifungal, böcek öldürücü, tıbbi ve kozmetik uygulamalarında yaygın olarak kullanılmaktadır. Bu çalışmadaki amacımız, Trakya bölgesinde yetişen Genista carinalis Griseb. (Fabaceae) bitkisinin uçucu bileşenlerini GC-MS yöntemi ile araştırmak ve ham ekstrelerinde antibakteriyel aktivitesini belirlemektir. G. carinalis bitkisinden elde edilen n-hekzan ekstresinin uçucu bileşenleri, GC-MS yöntemi kullanılarak araştırıldı. G. carinalis'in uçucu bileşenleri üzerine yaptığımız araştırma sonucunda elli iki adet biyoaktif bileşen tespit edilmiştir. Çalışmada denenen hem Gram-pozitif hem de Gram-negatif bakteriler üzerinde en yüksek antibakteriyel etki G. carinalis’in etil asetat ekstresi ile elde edilmiştir. Bu ekstrenin Gram-pozitif bakterilere etkisinn Gram-negatiflere oranla daha yüksek olduğu gözlenmiştir.

Anahtar Kelimeler

Genista, Genista carinalis, Fabaceae, Uçucu yağ, Antibakteriyel aktivite

Kaynakça

• [1] D. Runyoro, M. Matee, N. Olipa, C. Joseph, H. Mbwambo, “Screening of Tanzanian medicinal plants for anti-candida activity,” BMC Complementary and Alternative Medicine, vol. 6, pp. 1-10, 2006.
• [2] L. Ercan, M. Dogru, “Determination of activity of Nasturtium officinale and its content of volatile organic compounds and fatty acids,” Journal of Agriculture and Nature, vol. 25, pp.11-21, 2022.
• [3] A. Garrido, L.A. Atencio, R. Bethancourt, A. Bethancourt, H. Guzman, M. Gutiérrez, A.A. Durant-Archibold, “Antibacterial activity of volatile organic compounds produced by the octocoral-associated bacteria Bacillus sp. BO53 and Pseudoalteromonas sp. GA327,” Antibiotics, vol. 9, no. 12, pp. 923, 2020.
• [4] E. Celik, G.Y. Celik, “Bitki uçucu yağlarının antimikrobiyal özellikleri,” Orlab On-Line Mikrobiyoloji Dergisi, vol. 5, no. 2, pp. 1-6, 2007.
• [5] F. Bakkali, S. Averbeck, D. Averbeck, M. Idaomar, “Biological effects of essential oils-A review,” Food and Chemical Toxicology, vol. 46, no. 2, pp. 446-475, 2008.
• [6] R. Laxminarayan, A. Duse, C. Wattal, A.K.M. Zaidi, H.F.L. Wertheim, N. Sumpradit, E. Vlieghe, G.L. Hara, I.M. Gould, H. Goossens, C. Greko, A.D. So, M. Bigdeli, G. Tomson, W. Woodhouse, E. Ombaka, A.Q. Peralta, F.N. Qamar, F. Mir, S. Kariuki, Z.A. Bhutta, A. Coates, R. Bergstrom, G.D. Wright, E.D. Brown, O. Cars, “Antibiotic resistance-the need for global solutions,” In The Lancet Infectious Diseases, vol. 13, no. 12, pp. 1057-1098, 2013.
• [7] S. Kırbag, E. Bagcı, “Picea abies (L.) Karst. ve Picea orientalis (L.) Link uçucu yağlarının antimikrobiyal aktivitesi üzerine bir araştırma,” Journal of Qafqaz University, vol. 3, no. 1, pp. 183-190, 2000.
• [8] T. Baytop, Türkiye’de Bitkiler ile Tedavi, Ankara, Türkiye: Nobel Tıp Kitapevleri, 1999.
• [9] P.E. Gibbs, Flora of Turkey and the East Aegean Islands, Edinburgh, UK: Edinburgh University Press, 1970.
• [10] T. Sabudak, H. Caliskan, H.H. Orak, M. Ozer, “Biological activity of new flavonoids and phenolic compounds from Cirsium italicum (Savi) DC.” Natural Product Research, vol. 35 no. 10, pp. 1613-1619, 2021.
• [11] K.K. Keshala, A.M.P.W. Bandara, C. Padumadasa, L.D.C. Peiris, “Bioactivities and GC-MS profiling of Malewana Madhumeha Choorna polyherbal hot infusion,” South African Journal of Botany, vol. 140, pp. 194-203, 2021.
• [12] D. Gulen, T. Sabudak, H.H. Orak, H. Caliskan, M. Ozer, “Bioactive compounds antibacterial and antifungal activities of two Cirsium species,” Acta Scientiarum Polonorum Hortorum Cultus, vol. 18, no. 5, pp. 213-221, 2019.
• [13] M. Balouiri, M. Sadiki, S.K. Ibnsouda, “Methods for in vitro evaluating antimicrobial activity: A review,” Journal of Pharmaceutical Analysis, vol. 6, no. 2, pp. 71-79, 2016.
• [14] N.A. Awadh Ali, B.K. Chhetri, N.S. Dosoky, K. Shari, A.J.A. Al-Fahad, L. Wessjohann, W.N. Setzer, “Antimicrobial, antioxidant and cytotoxic activitirs of Ocimum forskolei and Teucrium yemense (Lamiaceae) essential oils,” Medicines (Basel), vol. 4, no. 2, pp. 17, 2017.
• [15] T. Lograda, A.N. Chaker, P. Chalard, M. Ramdani, J.C. Chalchat, H. Silini, G. Figueredo, “Chemical composition and antimicrobial activity of essential oil of Genista numidica Spach. and G. saharae Coss et Dur,” Asian Journal of Plant Sciences, vol. 8, no. 7, pp. 495-499, 2009.
• [16] T. Lograda, A.N. Chaker, J.C. Chalchat, M. Ramdani, H. Silini, G. Figueredo, P. Chalard, “Chemical composition and antimicrobial activity of essential oils of Genista ulicina and G. vepres,” Natural Product Communications, vol. 5, no. 5, pp. 835-838, 2010.
• [17] D. Rigano, A. Russo, C. Formisano, V. Cardile, F. Senatore, “Antiproliferative and cytotoxic effects on malignant melanoma cells of essential oils from the aerial parts of Genista sessilifolia and G. tinctoria,” Natural Product Communications, vol. 5, no. 7, pp. 1127-1132, 2010.
• [18] B. Poorabbas, J. Mardaneh, Z. Rezaei, M. Kalani, G. Pouladfar, M.H. Alami, J. Soltani, A. Shamsi-Zadeh, S. Abdoli-Oskooi, M.J. Saffar, A. Alborzi, “Nosocomial infections: Multicenter surveillance of antimicrobial resistance profile of Staphylococcus aureus and Gram negative rods isolated from blood and other sterile body fluids in Iran,” Iranian Journal of Microbiology, vol. 7, no. 3, pp. 127-135, 2015.
• [19] C. Yavuz, D.D. Dereli Kılıç, A. Ayar, T. Yıldırım, “Antibacterial effects of methanol extracts of some plant species belonging to Lamiaceae family,” International Journal of Secondary Metabolite, vol. 4, no. 3, pp. 429-433, 2017.
• [20] Q. Zhang, C. Yue, Y. Zhang, Y. Lu, Y. Hao, Y. Miao, J. Li, Z. Liu, “Six metal-organic frameworks assembled from asymmetric triazole carboxylate ligands: Synthesis, crystal structures, photoluminescence properties and antibacterial activities,” Inorganica Chimica Acta, vol. 473, pp. 112-120, 2018.
• [21] N. Gugala, D. Vu, M.D. Parkins, R.J. Turner, “Specificity in the susceptibilities of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus clinical isolates to six metal antimicrobials,” Antibiotics, vol. 8, no. 2, pp. 51, 2019.
• [22] M.J. Kohoude, F. Gbaguidi, P. Agbani, M.A. Ayedoun, S. Cazaux, J. Bouajila, M. Eko, J. Kohoude, “Chemical composition and biological activities of extracts and essential oil of Boswellia dalzielii leaves,” Pharmaceutical Biology, vol. 55, no. 1, pp. 33-42, 2016.
• [23] T. Sharmin, R. Sultana, F. Hossain, S.K. Shakil, F. Hossen, M. Mamun, O. Rashid, “Neuropharmacological and antibacterial effects of the ethyl acetate extract of Diospyros malabarica (Ebenaceae) seeds,” Clinical Phytoscience, vol. 4, no. 1, pp. 1-8, 2018.
• [24] J.D. Tamokou, D.J. Simo Mpetga, P. Keilah Lunga, M. Tene, P. Tane, J.R. Kuiate, “Antioxidant and antimicrobial activities of ethyl acetate extract, fractions and compounds from stem bark of Albizia adianthifolia (Mimosoideae),” BMC Complementary and Alternative Medicine, vol. 12, no. 1, pp. 1-10, 2012.
• [25] B.T.S. Yff, K.L. Lindsey, M.B. Taylor, D.G. Erasmus, J.A. Jager, “The pharmacological screening of Pentanisia prunelloides and the isolation of the antibacterial compound palmitic acid,” Journal of Ethnopharmacology, vol. 79, no. 1, pp. 101-107, 2002.
• [26] D.A. Gado, M.A. Abdalla, A.S. Ahmed, B. Madikizela, S.M. Nkadimeng, M.M. Ehlers, L.J. McGaw, “In vitro antibacterial activity of Loxostylis alata extracts and isolated compounds against Salmonella species,” BMC Complementary Medicine and Therapies, vol. 21, no. 1, 1-16, 2021.
• [27] G. Casillas-Vargas, C. Ocasio-Malave, S. Medina, C. Morales-Guzman, R.G. Del Valle, N.M. Carballeira, D.J. Sanabria-Rios, “Antibacterial fatty acids: An update of possible mechanisms of action and implications in the development of the next-generation of antibacterial agents,” Progress in Lipid Research, vol. 82, no. 101093, 2021.