Multietiological abortion induced by Brucella melitensis and Chlamydophila abortus in a sheep fetus

Yıl 2023, Cilt: 34 Sayı: 2, 205 - 210, 28.12.2023

İbrahim Deniz Ertan Oruç

https://doi.org/10.35864/evmd.1289382

Öz

In this case, multietiological abortion due to Brucella melitensis (B. melitensis) and Chlamydophila abortus (C. abortus) agents was defined in a sheep abortion. An aborted sheep fetus submitted to Konya Veterinary Control Institute (KVCI) from a sheep farm in Nigde province in Brucella spp. presence was determined by bacterial isolation method and confirmed by slide agglutination test with monospecific A and M sera. C. abortus was detected by real time PCR and immunohistochemistry (IHC) methods. In histopathological examinations, in the lung; hyperemia, hemorrhage and bronchopneumonia, In the heart; in the placenta with degeneration and necrosis of heart muscle cells; hyperemia, hemorrhage, edema, necrosis and mononuclear cell infiltrations were observed. In this study, it was emphasized that in the fight against sheep abortions, multi-etiological abortions involving more than one factor should be taken into consideration, as well as an etiological agent, and it would be appropriate to use multidisciplinary diagnostic methods to determine the abortion factors, thus contributing to the prevention of abortions, which is still an important problem in sheep breeding.

Anahtar Kelimeler

Brucella melitensis, Chlamydophila abortus, Immunohistochemistry, Multietiological abortion, PCR

Destekleyen Kurum

There is no person/organization that financially supports the study and the authors do not have any conflict of interest.

Proje Numarası

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Teşekkür

We would like to thank the Ministry of Agriculture and Forestry General Directorate of Food and Control for permission to publish the case report and the Konya Veterinary Control Institute Directorate for their support.

Bir koyun fetusunda Brucella melitensis ve Chlamydophila abortus tarafından oluşturulan multietiyolojik abort

Öz

Bu olguda, Konya Veteriner Kontrol Enstitüsü Müdürlüğüne (KVKEM) 2021 yılında, Niğde ilindeki bir koyun işletmesinden gönderilen koyun abortunda Brucella melitensis (B. melitensis) ve Chlamydophila abortus (C. abortus) etkenlerine bağlı multietiyolojik abort tanımlandı. Brucella spp. varlığı bakteriyel izolasyon yöntemi ile belirlendi ve monospesifik A ve M serumları ile yapılan lam aglutinasyon testi ile doğrulandı. C. abortus ise real time PZR ve immunohistokimya (İHK) yöntemleri ile tespit edildi. Histopatolojik incelemelerde ise akciğerde; hiperemi, kanama ve bronkopnömoni, Kalpte; kalp kası hücrelerinde dejenerasyon ve nekroz ile plasentada; hiperemi, kanama, ödem, nekroz ve mononükleer hücre infiltrasyonları gözlendi. Bu çalışmanın, koyun abortları ile mücadelede tek bir etiyolojik ajanın yanında birden fazla etkenin karıştığı multietiyolojik abortların da dikkate alınması gerektiği, abort etkenlerini belirlemek için de multidisipliner teşhis yöntemlerinin kullanılmasının uygun olacağını belirtmek ve böylelikle koyun yetiştiriciliğinde halen önemli bir sorun olan abortların önlenmesine katkı sağlaması açısından yayımlanması uygun bulunmuştur.

Anahtar Kelimeler

Brucella melitensis, Chlamydophila abortus, Immunohistochemistry, Multietiological abortion, PCR

Proje Numarası

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Kaynakça

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