Coat proteın variability of Apple mosaic virus isolates from different plant hosts

Yıl 2019, Cilt: 3 Sayı: 2, 247 - 251, 23.12.2019

Filiz Ertunç

Öz

Apple mosaic
virus,  apathogen of stone and pome
fruits and hazelnut worldwide, shows great variability in its biological,
serological and molecular properties. Apple mosaic virus (ApMV) variants were
collected from hazelnut plantations located in Black Sea region of Turkey and
the apple isolates were collected from main apple cultivation areas. The
infection was present on different local hazelnut varieties but the infection
was only detected on Granny Smith apple variety in Turkey. The Rubus plantations surrounding the
orchards were also infected with ApMV. The coat protein sequences of fifteen
ApMV variants from different hazelnut varieties and the ‘Granny Smith’ apple variety
were obtained  and the phylogenetic
analyses  were performed. The sequences
obtained from hazelnut revealed slightly different in nucleic acid and amino
acid composition compared to the sequences obtained from apples in Turkey. Since
the Granny Smith is originated from abroad, the infection might be imported by
the infected plant material to Turkey.

Anahtar Kelimeler

Apple mosaic virus, apple, hazelnut, coat protein variability

Destekleyen Kurum

TUBITAK

Proje Numarası

106O447

Teşekkür

The authors are grateful to TUBITAK for supporting this research by the project coded 106O447 and Ankara University Biotechnology Institute for providing coat protein gene sequences of Turkish ApMV isolates.

Kaynakça

• Alrefai R, L.L. Domier, C. J. D’Arcy, S. S. Kobran 1995.The complete nucleotide sequence of apple mosaic virus RNA-3. Archives of Virology 140, 1247-1256.
• Ertunc F, S. Topkaya, A. Sezer, 2014. Distribution and molecular detection of apple mosaic virus in apple and hazelnut in Turkey. African Journal of Biotechnology 13, 3144-3149.
• Hall T. A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acid Symposium Series 41, 95-98.
• Grimova L, L. Winkowska, P. Rysanek, P. Svoboda, K. Petrzik, 2013. Reflects the coat protein variability of apple mosaic virus host reference. Virus Genes 47, 119-125.
• Kobylko T and B. Nowak, 2006. Detection and occurence of Apple mosaic virus in hazelnut in South-East Poland. Journal of Plant Pathology 122.
• Korkmaz, G., H.M. Sipahioğlu, M. Usta, 2013. Survey of Apple mosaic virüs in apple-growing provinces of East Anatolia (Malatya and Van) by RNA probe hybridization assay and RT-PCR. Turkish Journal of Agriculture and Forestry 37, 711-718.
• Lakshmi V, V. Hallan, R. Ram, N. Ahmed, A.A. Zaidi, A. Varma, 2011. Diversity of Apple mosaic virus isolates in India based on coat protein and movement protein genes. Indian Journal of Virology 22(1), 44-49. Lee P G, K.H. Ryu, H.R. Kim, D.W. Lee, J.S. Kim, M.H. Park, Y.M. Noh, S.H. Cho, D.H. Han, C.H. Lee, 2002. Cloning and phylogenetic characterization of coat protein genes of two isolates of Apple mosaic virus from ‘Fuji’ apple. Plant Pathology Journal 18, 259-265.
• Menzel N, W. Jelkmann, E. Maiss, 2002. Detection of four apple viruses by multiplex RT-PCR assays with coamplification of plant m-RNA as internal control. Journal of Virological Methods 99, 89-92.
• Nemeth M., 1996. Virus, mycoplasma and rickettsia diseases of fruit trees. Dordrecht, Netherlands, Martinus Nijhoff Publishers.
• Rott M. E. and W. Jelkmann, 2001. Characterization and detection of several filamentous viruses of cherry, adaptation of an alternative cloning method (DOP-PCR and modification of an RNA extraction protocol. European Journal of Plant Pathology 107, 411-420.
• Shiel P.J., R.H. Alrefai, L.L. Domier, S.S. Kobran, P.H. Berger, 1995. The complete sequence of Apple mosaic virus RNA-3. Archieves of Virology 140, 1247-1256.
• Tamura K, D. Peterson, N. Peterson, G. Stecher, M. Nei, S. Kumar, 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution 28, 2731-2739.
• Thompson J.D., TJ Gibson, F. Plewniak, F. Jeanmougin , D.G. Higgins, 1997. The Clustal_ X: Windows interface flexible stratejies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25 (24), 4876-4882.
• Tombisana T, T. Rana, V. Hallan, R. Ram, A.A. Zaidi, 2009. Molecular characterization of the Indian Strain of Apple mosaic virus isolated from apple (Malus domestitica. Phytoparasitica 37, 375-379.
• TUİK, 2017. Agricultural statistics of Turkey, database.(http://tuik.gov.tr)
• Van Regenmortel M. H. V., C.M. Fauquet, D.H.L. Bishop, E.B. Cartens, M.K. Estes, M.K. Lemon, J. Maniloff , M.A. Mayo, D.J. McGeoch, C.R. Pringle, R.B. Wickner, 2000. Virus Taxonomy, Seventh Report of the International Committee on Taxonomy of Viruses. Academic Press