Secondary metabolite estimation and antioxidant potential assessment of purple bell Thunbergia erecta (Benth.) T. Anderson

Yıl 2024, Cilt: 11 Sayı: 1, 23 - 36, 05.02.2024

Nitin Kochar Jayshree Vyas Khushbu Vyas Anil Chandewar Dharmendra Mundhada

https://doi.org/10.21448/ijsm.1247610

Öz

To quantify the aqueous and methanolic extracts for primary and secondary metabolites, and the antioxidant potential of leaf extracts of the Thunbergia erecta plant and to adopt them in Ayurvedic medications for various illnesses. Primary metabolites like carbohydrates, proteins, and secondary metabolites such as flavonoids, alkaloids, total phenols, and tannins were estimated using standard procedures. The 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), hydrogen peroxide, and phosphomolybdenum free radical scavenging activities were employed to evaluate the antioxidative potential. The phytochemical examination of T. erecta exhibited the presence of carbohydrates, proteins, amino acids, alkaloids, total polyphenolics, flavonoids, and tannins in significant quantity. The in vitro antioxidant potential of the species, clearly verifies that methanolic extract shows prominent antioxidant properties followed by the hydroalcoholic extract. From this study, it can be concluded that this species is effective in scavenging free radicals and may be a powerful antioxidant. The T. erecta leaf extract showed the existence of bioactive components which are known to exhibit medicinal activities. The findings of this study suggest that these plant leaves could be a potential source of natural antioxidants that could have great importance as therapeutic agents in preventing various diseases.

Anahtar Kelimeler

Antioxidant activity, Phytochemicals, Primary metabolites, Secondary metabolites, Thunbergia erecta

Kaynakça

• Atere, T.G., Akinloye, O.A., Ugbaja, R.N., Ojo, D.A., & Dealtry, G. (2018). In vitro antioxidant capacity and free radical scavenging evaluation of standardized extract of Costus afer leaf. Food Science and Human Wellness, 7(4), 266 272. https://doi.org/10.1016/j.fshw.2018.09.004
• Begum, A., Hossen, A., Moly, A.A., Bhuiyan, Md.M.R., & Shahed-Al-Mahmud, Md.S.-A.-M. (2019). In Vivo Sedative and Anxiolytic Activities of Thunbergia erecta (Acanthaceae) Leaves Activate Gamma-Aminobutyric Acid (GABA) Mediated Hyperpolarization in Swiss Albino Mice. Pharmacology & Pharmacy, 10(04), 177 193. https://doi.org/10.4236/pp.2019.104016
• Chan, E.W.C., & Lim, Y.Y. (2006). Antioxidant Activity of Thunbergia Laurifolia Tea. Journal of Tropical Forest Science, 18(2), 130–136.
• Chandel, V., Choubey, A., & Asati, S. (2020). Evaluation of In Vitro Antiurolithiatic activity of Thunbergia erecta. Plant Archives, 20(2), 4295–4299.
• Daniel Geedhu, S., & Krishnakumari S. (2014). Quantitative Analysis of Primary and Secondary Metabolites in Aqueous Hot Extract of Eugenia uniflora (L.) Leaves. 2015, 8(1), 334–338.
• Darwish, W.S., Khadr, A.E.S., Kamel, M.A.E.N., Abd Eldaim, M.A., El Sayed, I.E.T., Abdel-Bary, H. M., Ullah, S., & Ghareeb, D.A. (2021). Phytochemical Characterization and Evaluation of Biological Activities of Egyptian Carob Pods (Ceratonia siliqua L.) Aqueous Extract: In Vitro Study. Plants, 10(12), 2626. https://doi.org/10.3390/plants10122626
• Das, K., Dang, R., Sivaraman, G., & Ellath, R.P. (2018). Phytochemical Screening for Various Secondary Metabolites, Antioxidant, and Anthelmintic Activity of Coscinium fenestratum Fruit Pulp: A New Biosource for Novel Drug Discovery. The Turkish Journal of Pharmaceutical Sciences, 15(2), 156–165. https://doi.org/10.4274/tjps.54376
• Doraiswamy, R., & Saminathan, V. (2020). Phytochemical analysis, antioxidant and anticancer activities of durian (Durio zibethinus Murr.) fruit extract. Journal of Research in Pharmacy, 24(6), 882–892. https://doi.org/10.35333/JRP.2020.247
• El-Din, M.I.G., George, M.Y., & Youssef, F.S. (2023). Chemical characterization of the polyphenolic rich fraction of Thunbergia erecta and its therapeutic potential against doxorubicin and cyclophosphamide-induced cognitive impairment in rats. Journal of Ethnopharmacology, 307, 116213. https://doi.org/10.1016/j.jep.2023.116213
• Ganguly, S., Kumar, J., & Seal, T. (2021). Characterization of secondary metabolites in different parts of Ocimum gratissimum L. by in vitro antioxidant activity and high-performance liquid chromatography–diode-array detector analysis. Pharmacognosy Magazine, 17(74), 209–215. https://doi.org/10.4103/pm.pm_550_20
• Hayat, J., Akodad, M., Moumen, A., Baghour, M., Skalli, A., Ezrari, S., & Belmalha, S. (2020). Phytochemical screening, polyphenols, flavonoids, and tannin content, antioxidant activities, and FTIR characterization of Marrubium vulgare L. from 2 different localities of Northeast of Morocco. Heliyon, 6(11), e05609. https://doi.org/10.1016/j.heliyon.2020.e05609
• Hidayati, J.R., Yudiati, E., Pringgenies, D., Oktaviyanti, D.T., & Kusuma, A.P. (2020). Comparative Study on Antioxidant Activities, Total Phenolic Compound and Pigment Contents of Tropical Spirulina platensis, Gracilaria arcuata and Ulva lactuca Extracted in Different Solvents Polarity. E3S Web of Conferences, 147, 03012. https://doi.org/10.1051/e3sconf/202014703012
• Jain, V., Karibasappa, G., Dodamani, A., & Mali, G. (2017). Estimating the carbohydrate content of various forms of tobacco by the phenol-sulfuric acid method. Journal of Education and Health Promotion, 6(1), 90. https://doi.org/10.4103/jehp.jehp_41_17
• Kaur, A., Kaur, M., Kaur, P., Kaur, H., Kaur, S., & Kaur, K. (2015). Estimation and Comparison of Total Phenolic and Total Antioxidants in Green Tea and Black Tea. Global Journal of Bio-Science and Biotechnology, 2015, 4(1), 116–120.
• Khandelwal, K. (2008). Practical Pharmacognosy, Techniques and Experiment (Nineteenth Edition). Nirali Prakashan.
• Khorasani Esmaeili, A., Mat Taha, R., Mohajer, S., & Banisalam, B. (2015). Antioxidant Activity and Total Phenolic and Flavonoid Content of Various Solvent Extracts from In Vivo and In Vitro Grown Trifolium pratense L. (Red Clover). BioMed Research International, 2015, 1 11. https://doi.org/10.1155/2015/643285
• Kochar, N.I., Vyas, J.C., Vyas, K.B., & Chandewar, A.V. (2023). An Ethnobotanical and Phyto-Pharmacological Review on a MultifacetedOrnate Flowering Plant ‘Thunbergia erecta’. Current Traditional Medicine, 9(4), e190822207750. https://doi.org/10.2174/2215083808666220819155700
• Kusimo, M.O., Ukoha, H., Oludare, A., Afolabi, O., & Agwae, M. (2019). Halochromic properties and antimicrobial potential of crude extracts from five species of ornamental plants. UNED Research Journal, 11(3), 283–291. https://doi.org/10.22458/urj.v11i3.2586
• Li, L.S., Chiroma, S.M., Hashim, T., Adam, S.K., Mohd Moklas, M.A., Yusuf, Z., & Rahman, S.A. (2020). Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract. Heliyon, 6(6), e04141. https://doi.org/10.1016/j.heliyon.2020.e04141
• Maduka, E.A., Yelwa, J.M., Honda, T.J., Ebunu, A.I., & Muhammad, A. (2020). Physio-Chemical Studies of Isoberliniadoka Seedoil for Possible use as Edible Oil. ARC, 7(8), 11–19.
• Mandal, S., Patra, A., Samanta, A., Roy, S., Mandal, A., Mahapatra, T.D., Pradhan, S., Das, K., & Nandi, D.K. (2013). Analysis of the phytochemical profile of Terminalia arjuna bark extracts with antioxidative and antimicrobial properties. Asian Pacific Journal of Tropical Biomedicine, 3(12), 960–966. https://doi.org/10.1016/S2221-1691(13)60186-0
• Maswada, H.F. (2013). Assessment of Total Antioxidant Capacity and Antiradical Scavenging Activity of Three Egyptian Wild Plants. Journal of Medical Sciences, 13(7), 546 554. https://doi.org/10.3923/jms.2013.546.554
• Mishra, B., Kar, D.M., Maharana, L., & Mishra, G.P. (2016). Physicochemical and phytochemical investigation of different fractions from hydroalcoholic extract of Tectona grandis (Linn) barks. Scholars Research Library, 8(4), 80–85.
• Moonmun, D., Majumder R., & Lopamudra A. (2017). Quantitative Phytochemical Estimation and Evaluation of Antioxidant and Antibacterial Activity of Methanol and Ethanol Extracts of Heliconia rostrata. Indian Journal of Pharmaceutical Sciences, 79(01), 79 90. https://doi.org/10.4172/pharmaceutical-sciences.1000204
• Pinchuk, I., Shoval, H., Dotan, Y., & Lichtenberg, D. (2012). Evaluation of antioxidants: Scope, limitations, and relevance of assays. Chemistry and Physics of Lipids, 165(6), 638–647. https://doi.org/10.1016/j.chemphyslip.2012.05.003
• Rahim, N.A., Zakaria, N., Dzulkarnain, S.M.H., Azahar, N.M.Z.M., & Abdulla, M.A. (2017). Antioxidant activity of alstonia Angustifolia ethanolic leaf extract. AIP Conf. Proc. 1891, 020012. https://doi.org/10.1063/1.5005345
• Refaey, M.S., Abdelhamid, R.A., Elimam, H., Elshaier, Y.A.M.M., Ali, A.A., & Orabi, M.A.A. (2021). Bioactive constituents from Thunbergia erecta as potential anticholinesterase and anti-ageing agents: Experimental and in silico studies. Bioorganic Chemistry, 108, 104643. https://doi.org/10.1016/j.bioorg.2021.104643
• Saeed, N., Khan, M.R., & Shabbir, M. (2012). Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complementary and Alternative Medicine, 12(1), 221. https://doi.org/10.1186/1472-6882-12-221
• Senguttuvan, J., Paulsamy, S., & Karthika, K. (2014). Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities. Asian Pacific Journal of Tropical Biomedicine, 4(1), S359 S367. https://doi.org/10.12980/APJTB.4.2014C1030
• Singh, S.K., Patel, J.R., & Dangi, A. (2019). Physicochemical, Qualitative and Quantitative Determination of Secondary Metabolites and Antioxidant Potential of Kalanchoe Pinnata (Lam.) Pers. Leaf Extracts. Journal of Drug Delivery and Therapeutics, 9(1), 220 224. https://doi.org/10.22270/jddt.v9i1.2225
• Sulekha Rani, & Priti. (2019). Estimation of Total Alklaoids in Wild and In-vitro Regenerated Tinospora cordifolia. International Journal of Pharmaceutical Sciences and Research, 10(6), 2777–2784. https://doi.org/10.13040/IJPSR.0975-8232.10(6).2777-84
• Unuofin, J.O., Otunola, G.A., & Afolayan, A.J. (2017). Phytochemical screening and in vitro evaluation of antioxidant and antimicrobial activities of Kedrostis africana (L.) Cogn. Asian Pacific Journal of Tropical Biomedicine, 7(10), 901 908. https://doi.org/10.1016/j.apjtb.2017.09.008