Effect Of Piroxicam On Ileal Anastomoses In Wistar Albino Rats

Yıl 2011, Cilt: 1 Sayı: 2, 20 - 23, 25.11.2011

Elif Özkök Ahmet Kızmaz Bedia Çakmakoğlu Hülya Yılmaz Aydoğan İlhan Yaylım Eraltan Oğuz Öztürk Makbule Aydın

Öz

Objective: The purpose of our study was to examine the influence of piroxicam, non-steroid anti-inflammatory drug, on healing of ileal anastomoses in rats.

Materials and Methods: 20 rats were divided randomly into two groups and submitted to resection and ileal anastomosis. The test group received piroxicam, 2mg/kg/day, beginning one day before operation until sacrification. The animals were sacrificed on third and seventh days after the operation. Tissue homogenate, indicator of tissue collagen content, was hydrolyzed with HQ
and then evaporated to dryness. Evaporated sample was deproteinized in HC1 and after this processes hydroxyprolin was measured spectrophotometrically in supernatant fraction in which taken isopropanol.

Results: In test group, hydroxyproline concentrations were slightly reduced on 3r d day but again increased to the preoperational levels on 7th day after anastomosis (p> 0.05). On the other hand, in the control group, ileal hydroxyproline concentrations were reduced significantly on 3rd day but increased to preoperational levels on 7t h day after the operation.

Conclusion: In conclusion, piroxicam might improve hydroxyproline levels during initial phase of wound healing. 

Anahtar Kelimeler

Piroxicam, Anastomoses, Wistar Albino Rats

Wistar Albino Sıçanlarda Ileal Anastomoz iyileşmesinde Piroksikamın Etkisi

Öz

Amaç: Çalışmamızın amacı bir non steroid antiinflamatuar ilaç olan piroksikamın sıçanlarda ileal anastomoz iyileşmesindeki
etkisini incelemekti.

Gereç ve Yöntem: 20 sıçan randomize olarak iki gruba ayrılarak rezeksiyonlan ve ileal anastomozlan yapıldı. Deney grubuna
operasyondan bir gün önceden sakrifiye edilene kadar 2mg/kg/gün piroksikam verildi. Hayvanlar operasyondan sonraki üçüncü
ve yedinci günlerde sakrifiye edildi. Dokunun kollajen miktarını göstergesi olarak doku homojenatı HC1 içinde hidroliz edilmesine
takiben evapore edildi. Evapore edilmiş örnek HC1 içinde deproteinize edilerek işlem sonrasında süpernatan fraksiyonu isopropanol
içine alınarak hidroksiprolin spektrofotometrik olarak tayin edildi.

Bulgular: Deney grubunda hidroksiprolin konsantrasyonları üçüncü günde hafif düşerken anastomoz sonrası yedinci günde
operasyon öncesi seviyeye yükseldi. Diğer yandan kontrol grubunda ileal hidroksiprolin konsantrasyonu üçüncü günde anlamlı
olarak azalırken operasyon sonrasındaki yedinci günde operasyon öncesi seviyeye yükseldi.

Tartışma: Sonuç olarak piroksikam yara iyileşmesinin başlangıç fazı süresince hidroksiprolin düzeylerini arttırabilmektedir

Kaynakça

• Bergman I , Loxely R. Two improved and simplified methods for the spectrophotometric determination of hydroxyproline. Anal Chem 1963; 35: 1961-1965.
• Brasken P, Lehto M, Ren vail S. Changes in the connective tissue composition of the submucosal layer of colonic anastomosis. An immunohistologic study in rats. Acta Chir Scand 1989; 155: 413-419.
• Brogden RN, Heel RC, Speight TM, Avery GS. Piroxicam. A reappraisal of its pharmacology and therapeutic efficacy. Drugs 1984; 28: 292-323.
• Cronin K, Jackson DS, Dunphy JE. Changing bursting strength and collagen content of the healing colon. Surg Gynecol Obstet 1968; 126: 747-753.
• Goligher JC, Graham NG, Dombai DE FT. Anastomotic dehiscence after anterior resection of rectum and sigmoid. Br J Surg 1970; 57: 109-118.
• Hawley PR. Causes and prevention of colonic anastomotic breakdown. Dis Colon Rectum 1973 ; 16: 272-277.
• Hendriks T, Vereecken TH, Hesp WL, Schillings PH, de Boer HH. Loss of collagen from experimental intestinal anastomoses: early events. Exp Mol Pathol 1985; 42: 411¬418.
• Hendriks T, Mastboom WJ. Healing of experimental intestinal anastomoses. Parameters for repair. Dis Colon Rectum 1990; 33: 891-901.
• Hendricks JM, Hubens G, Wuyts FL, et al. Experimental study of intraperitoneal suramin on the healing of colonic anastomoses. Br J Surg 1999; 86: 1171-1175.
• Hesp FL, Hendriks T, Lubbers EJ, de Boer HH. Wound healing in the intestinal wall: A comparison between experimental ileal and colonic anastomoses. Dis Colon Rectum 1984; 27: 99-104.
• Higgs GA, Vane JR. Inhibition of cyclo-oxygenase and lipoxygenase. Br Med Bull 1983; 39: 265-270. .
• Hunt TK, Hawley PR, Hale J, Goodson W, Thakral KK. Colon repair: The collagenous equilibrium. In: Hunt TK (Editor). Wound healing and wound infection. Theory and surgical practice. New York: Appleton-Century-Crotts, 1980; 153-159.
• Irvin TT, Goligher JC. Aetiology of disruption of intestinal anastomoses. Br J Surg 1973; 60: 461-464.
• Irvin TT, Hunt TK. Reapprasial of the healing process of anastomosis of the colon. Surg Gynecol Obstet 1974; 138: 741-746.
• Jiborn H, Ahonen J, Zederfeldt B. Healing of experimental colonic anastomoses. I . Bursting strength of the colon after left colon resection and anastomosis. Am J Surg 1978; 136: 587-594.
• Jiborn H, Ahonen J, Zederfeldt B. Healing of experimental colonic anastomoses. II. Breaking strength of the colon after left colon resection and anastomosis. Am J Surg 1978;136: 595- 599.
• Jiborn H, Ahonen J, Zederfeldt B. Healing of experimental colonic anastomoses. III. Collagen metabolism in the colon after left colon resection. Am J Surg 1980; 139: 398- 405.
• Jonsson K, Jiborn H, Zederfeldt B. Breaking strength of small intestinal anastomoses. Am J Surg 1983; 145: 800¬803.
• Mann B, Kleinschmidt S, Stremmel W. Prospective study of hand-sutured anastomosis after colorectal resection. Br J Surg 1996; 83:29-31.
• Mastboom WJ, Hendriks T, van Elteren P, de Boer HH. Piroxicam affects collagen changes around experimental intestinal anastomoses. Eur Surg Res 1989; 21: 305-312.
• Mastboom WJ, Hendriks T, van Elteren P, de Boer HH. The influence of non-steroid anti-inflammatory drugs on experimental intestinal anastomoses. Dis Colon Rectum 1991; 34: 236-243.
• Morgenstern L, Yamakawa T, Ben-Shoshan M, Lippman H. Anastomotic leakage after low colonic anastomosis. Clinical and experimental aspects. Am J Surg 1972;123: 104-109.
• Pakkastie TE, Luukkonen PE, Jarvinen HJ. Anastomotic leakage after anterior resection of the rectum. Eur J Surg 1994; 160: 293-297.
• Stromberg BV, Klein L. Collagen formation during the healing of colonic anastomoses. Dis Colon Rectum 1982; 25: 301-304.
• Stromberg BV, Klein L. Localized healing of small intestinal anastomoses. Am Surg 1982; 48: 579-581.
• van der HAMAC, KortWJ,WeijmaIM, JeekelH. Transient protection of incomplete colonic anastomoses with fibrin sealant: an experimental study in the rat. J Surg Res 1993; 55: 256-260.
• Wise L, McAlister W, Stein T, Shuck P. Studies on the healing of anastomoses of small and large intestines. Surg Gynecol Obstet 1975; 141: 190-194.