Multiplication Of Seed Mini Tubers From In Vitro Potato Plantlets

Yıl 2017, Cilt: 11 Sayı: 3, 39 - 41, 31.12.2017

Güngör Yılmaz Abdurrahman Caglı Yasin B. Karan Uğur Pırlak

Öz

The promising clones developed in the last stage of potato breeding should be produced with the meristem culture required for the registration experiments. Production is required to obtain a small number of seeds from the in vitro plantlets produced by the meristem culture. This investigation carried out two different locations (Nigde-Turkey and Tokat-Turkey) in 2016. The aim of this study is to determine in which medium the tubers from the in vitro plantlets can be determined faster and to obtain seeds with suitable characteristics. In this study, 16 different promising clones were used. Plants from in vitro plantles of these clones were compared in pot and soil conditions under net greenhouse conditions. A mixture of 2/3 peat and 1/3 perlite was used as a medium in the pots.

According to the findings, the number of tubers per pot and the number of tubers per plant was higher in pots condition in the net greenhouse than in the field condition in the net greenhouses whereas in the field condition in the net greenhouses, the number of tubers per plant and the number of tubers were less than, although the number of plants planted per unit area was higher. Therefore, it has been determined that more space is required for the amount of seeds required for location experiments.

While the average number of tubers per plant from the tubers planted directly on the soil in the net greenhouses ranged from 2.4 to 5.4, the number in the net greenhouse field condition ranged from 5.7 to 14.1. Similarly, while tuber yield per plant in the field condition ranged from 123.1 to 383.6 g, in the peat-pearlite pot medium ranged from 290.3 to 470.9 g. It was also determined that the tuber seeds produced in pots and peat-perlite medium are more homogenous and healthier. As a result of the study, it was determined that the production of the tubers from the in vitro plantlets in peat-pearlite pots in the net-greenhouse condition is better results than other condition in net greenhouse. It has been determined that more area is required for direct field planting the net greenhouse to produce the same amount of seed nodules.

Anahtar Kelimeler

potato, mini tuber, multiplication of mini tuber, net greenhouse

Kaynakça

• [1]. Anonymous, 2015. Potato Production Yearbook. http://www. fao.org/statistical/
• [2]. Anonim, 2017. Türkiye’ninMilliÇeşitListesi, TarlaBitkileri. http://www.ttsm.gov.tr/
• [3]. Novak, F.J., Zadina, J., Horackova, V. Maskova, I., 1980. The effect of gowth regulators on meristem tip development and in vitro multiplication of Solanum tuberosum L. plants. Potato Research. 23(2): 155-166.
• [4]. Farran, I., Mingo-Castel, A.M., 2006. Potato minituber production using aerophonics: effect of plant density and harvesting intervals. American Journal of Potato Research. 83: 47-53
• [5]. Almekinders CJM, ChujoyEThiele G., 2009. The use of True Potato Seed as a pro-poor technology: The efforts of an International Agricultural Research Institute to innovating potato production. Pot Res. 52: 275-293.
• [6]. Yılmaz, G. Kandemir, N., Yanar, Y. Kınay, A. 2017. Üstün Özelliklere Sahip Patates Genotipleri Kullanılarak Melez Klonların Elde Edilmesi ve Bazı Yerel Patates Çeşitlerinin Islahı. TÜBİTAK-TOVAG 113O928 Nolu proje sonuç raporu.
• [7]. Struik PC, Wiersema SG., 1999. Seed potato technology. Wageningen University Press. The Netherlands.
• [8]. Yılmaz, G. 2016. “Türkiye’de Nişasta ve Şeker Bitkileri Üretimi ve Sektörün Geliştirilmesine Dair Yaklaşımlar”. 2023-2071 Vizyonuyla Tarım. Sayfa: 226-245. TOÇ-BİR SEN. ISBN 978605-85250-1-6. Ankara
• [9]. Otazu, V., 2008. Quality seed potato production using aeoponics. A potato production manual. Centro Internacional de la Papa, Lima, Peru.
• [10]. Rykaczewska, K., 2017. Impact of heat and drought stresses on size and quality of the potato yield. Plant Soil Environ. Vol. 63, 2017, No. 1: 40–46.
• [11]. Levy D., Veilleux R.E., 2007. Adaptation of potato to high temperatures and salinity – A review. American Journal of Potato Research, 84: 487–506.
• [12]. Haverkort A.J. Verhagen, A., 2008. Climate Change and its Repercussions for the Potato Supply Chain. Potato Research, Vol. 51, No. 3-4, pp. 223-237.
• [13]. Bodlaender, K.B.A, 1963. Influence of Temperature, Radia- tion and Photoperiod on Development and Yield. In: J. D. Ivins and F. L. Milthorpe, Eds., Growth of the Potato, Butterworths, London, 1963, pp. 199-210.
• [14]. Van Dam, J., Kooman P. L., Struik P. C., 1996. Effects of Temperature and Photoperiod on Early Growth and Final Number of Tubers in Potato (Solanum tuberosum L). Potato Research, Vol. 39, No. 1, pp. 51-62.
• [15]. Rykaczewska, K., 2013. The Impact of High Temperature during Growing Season on Potato Cultivars with Different Response to Environmental Stressses. American Journal of Plant Sciences. 4, 2386-2393.
• [16]. Negash, K., 2014. Rapid Multiplication Techniques (RMTs): A Tool for the Production of Quality Seed Potato (Solanum Tuberosum L.) in Ethiopia. Assian Journal of Crop Science 6 (3): 176-185.
• [17]. Karan, Y.B., Yılmaz, G. 2016. Effects of Different Minituber Size and Planting Density on Yield and Yield Components of BasciftlikBeyazi Local Potato Cultivar. Journal of New Results in Science 11, 64-69.