Effects of 6-benzylaminopurine and Activated Carbon on Indirect Organogenesis of Fritillaria Imperialis

Yıl 2020, Cilt: 23 Sayı: 2, 297 - 303, 30.04.2020

Nigar Saeed , Sabahattin Cömertpay

https://doi.org/10.18016/ksutarimdoga.vi.627056

Cited By: 1

Öz

Fritillaria imperialis needs 2-3 years to regenerate from seeds, which makes its production
quite challenging. In this study, effects of three concentrations of
6-Benzylaminopurine (BAP) (0.00, 0.10 and 0.15 mg/L) and three different
amounts (0.0, 3.0 and 3.5 g/L) of activated carbon (AC) on the growth of F.
imperialis plantlets in vitro were determined. The auxins were at
similar concentration (0.01 mg/L of NAA and 0.01 mg/L of IAA) for all
treatments. It has been found that the size and number of bulblets, as well as
root and shoot numbers in regenerated plantlets were increased significantly
(p<0.05) by adding 3.5 g/L of AC in culture medium supplemented with 0.10
mg/L of BAP. Moreover, addition of AC significantly reduced the length of roots
in all doses studied (p<0.05). The supreme concentration of BAP (0.15 mg/L)
decreased the bulblet number in all treatments, however, when supplemented with
3.5 g/L of AC, it was found to raise all the values measured (p<0.05).
Overall, the best growth of the plant parts from callus were achieved with the
highest concentration of AC and 1.5 mg/L of BAP.

Anahtar Kelimeler

Activated charcoal, Bulblet, Crown imperial, Cytokinin, Micropropagation

Destekleyen Kurum

Kahramanmaraş Sütçü İmam Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

2017/4-28D

Teşekkür

This work was supported by Scientific Research Projects (BAP) Unit of Kahramanmaras Sutcu Imam University with the project number of 2017/4-28D.

6-benzilaminopurin ve Aktif Karbonun Fritillaria Imperialis'in Dolaylı Organogenezi Üzerindeki Etkileri

Öz

Fritillaria imperialis türü bitkiler, tohumlarını
yenilemek için iki yıldan fazla bir süreye ihtiyacı duymakta ve bu durum
üretimlerini zorlaştırmaktadır. Bu çalışmada, üç farklı derişimdeki
6-Benzilaminopurinin (BAP) (0.00, 0.10 ve 0.15 mg/L) ve üç farklı miktardaki
(0.0, 3.0 ve 3.5 g/L) aktif karbonun (AC) F.
imperialis bitkilerinin büyümesine etkisi in vitro olarak
incelenmiştir. Tüm muamele grupları için benzer konsantrasyonda oksin (0.01
mg/L NAA ve 0.01 mg/L IAA) kullanılmıştır. 0.10 mg/L BAP ilave edilmiş kültür
ortamlarına 3.5 g/L AC eklenmesinin bitkilerin yumru çap uzunluğu ve sayısının
yanında kök ve sürgün sayılarını istatiksel olarak anlamlı bir şekilde (p<0.05) arttırdığı
gözlenmiştir. Dahası, AC eklenmesi, çalışılan tüm dozlarda kök uzunluklarını
önemli ölçüde azaltmıştır (p<0.05). Yüksek BAP konsantrasyonunun (0.15 mg/L)
çalışılan tüm grupların yumru sayılarını azalttığı, ancak 3.5 g/L AC ile
desteklendiğinde ölçülen tüm değerlerin artışını sağladığı bulunmuştur
(p<0.05). ).
Genel olarak, kallustan bitki kısımlarının en iyi şekilde büyümesi, en yüksek
konsantrasyonda AC ve >1.5 mg/L BAP ile elde
edilmiştir.

Anahtar Kelimeler

Aktif karbon, yumru, ters lâle, sitokinin, mikropropagasyon

Proje Numarası

2017/4-28D

Kaynakça

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