Dermatofitlerin İzolasyonu, Moleküler Tanımlanması ve Bununla İlişkili Bazı Çevresel Faktörlerin İncelenmesi

Yıl 2023, Cilt: 1 Sayı: 1, 1 - 9, 30.06.2023

Murad Khamees Mushıb Waleed Khalid Ahmed Muhammet Gaffaroğlu

Öz

Bu çalışmanın amacı, dermatofitlerin laboratuvarda fungal test yöntemleriyle izole edilmesi
ve teşhis edilmesi, ortaya çıkan türlerin tanımlanması ve mevcut yerel izolatların Ulusal
Biyoteknoloji Bilgi Merkezi (NCBI) tarafından sağlananlarla karşılaştırılmasıdır.
Dermatofit izolatları, kolonilerinin makroskopik ve mikroskopik özellikleri incelenerek
tanımlanmıştır. Teşhislerinin doğruluğundan emin olmak için, PCR tekniği kullanılarak
elde edilen sonuçlar, primerlerin (ITS1, ITS4) incelenen mantarların genomlarını
çoğalttığını ve çoğaltılan bantların (600 baz çifti) arasında değiştiğini göstermiştir. Bu
çalışmadaki 1.2.3.4 numaralı izolatlar, izolatlarla benzerlik göstermektedir. Genbank'ta
OP752127 numarasıyla kayıtlı Candida albicans. 5.6.7.8.9 numaralı izolatlar da OP752433
numaralı GenBank'ta kayıtlı Candida glabrata izolatları ile benzerlik göstermektedir.
10.11.12 numaralı türler OP712459 numaralı GenBank'ta kayıtlı Candida tropicalis
izolatları ile benzerlik göstermektedir. 13.14.15 ve 16 numaralı izolatlar GenBank
OP712621'de kayıtlı Trichophyton mentagrophytes izolatları ile benzerlik göstermektedir.
17 ve 18 numaralı izolatlar da Scopulariopsis brevicaulis ile benzerlik göstermektedir ve
Genbank OP752128'de kayıtlıdır.

Anahtar Kelimeler

Moleküler Tanımlama, Dermatofitler, PCR

Kaynakça

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