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Siyah Sarımsak Ekstraktının Ratlarda Deneysel Mide Ülseri Modelinde Koruyucu Etkilerinin Araştırılması

Yıl 2024, Cilt: 17 Sayı: 1, 62 - 67, 31.03.2024
https://doi.org/10.30607/kvj.1416456

Öz

Bu çalışmada gastrik ülserin önlenmesinde siyah sarımsak ekstraktının etkilerinin değerlendirilmesi amaçlandı. Bu amaçla 2-3 aylık 28 adet Spraque dawley ırkı rat rastgele olarak 4 gruba ayrıldı. Kontrol grubu (CG), Ülser grubu (UG), Ülser + pantoprazole tedavi grubu (PTG) ve Ülser + Siyah sarımsak grubu (BGG). Kontrol grubu hariç diğer gruplara 25 mg.kg-1 dozda indomethazin verilerek mide ülseri oluşturuldu. Daha sonra tedavi gruplarına Pantoprazol (20 mg.kg-1) ve siyah sarımsak eksraktı (275 mg.rat) uygulandı. Çalışma sonrasında mide örnekleri alınarak makroskobik (ülser skorlaması ve ülseratif alan mm2) ve histopatoloji incelemesi (HE boyaması) yapıldı. Biyokimyasal analizlerde oksidan/antioksidan parametre analizleri kapsamında malondialdehid (MDA, pg. ml-1) seviyesi ile süperoksid dismutaz (SOD, ng. ml-1) ve katalaz (CAT, ng. ml-1) aktiviteleri değerlendirildi. Elde edilen makroskobik sonuçlara göre; BGG ve UG arasında istatisliksel anlamlı değişimler bulundu (p<0.0001). PTG tüm gruplara göre daha iyi etki gösterdi. Histopatolojide; CG’de herhangi bir lezyona rastlanmadı. PTG ile CG arasında benzerlik vardı. UG’de yoğun kanama ve ülser odakları mevcutken, BGG’de sadece sınırlı fokal ülser bulundu. MDA’da değerler en yüksel UG’de görüldü. PTG ve BCG arasında istatisliksel anlam yoktu. SOD aktivitesi en düşük UG olup, yine PTG ve BGG’de benzerlik vardı. CAT aktivitesi en yüksek CG’de en düşük ise UG’de belirlendi. Sonuç olarak; tek ve yüksek dozda (%5 w.v, 275 mg.rat) uygulanan Siyah sarımsak ekstraktı mide ülserine karşı kısmi koruyucu etkinlik göstermiştir.

Kaynakça

  • Ahmed, T., & Wang, C. K. (2021). Black Garlic and Its Bioactive Compounds on Human Health Diseases: A Review.Molecules (Basel, Switzerland), 26(16),5028. doi.org/10.3390/molecules26165028
  • Badr, G. M., & Al-Mulhim, J.A. (2014). The protective effect of aged garlic extract on nonsteroidal anti-inflammatory drug-induced gastric inflammations in male albino rats, Evidence-based complementary and alternative medicine: eCAM, 759642. https://doi.org/10.1155/2014/759642
  • Boyacioglu, M., Kum, C., Sekkin, S., Yalinkilinc, H. S., Avci, H., Epikmen, E. T., & Karademir, U. (2016). The effects of lycopene on DNA damage and oxidative stress on indomethacin-induced gastric ulcer in rats, Clinical nutrition (Edinburgh, Scotland), 35(2), 428–435. https://doi.org/10.1016/j.clnu.2015.03.006
  • Boyacıoglu, M. (2019). Protective Effects of Silymarin on Indomethacin-Induced Gastric Ulcer in Rats, Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 16(2), 109-114.
  • Cantarella, G., Martinez, G., Cutuli, V. M., Loreto, C., D'Alcamo, M., Prato, A., Clementi, G. (2005). Adrenomedullin modulates COX-2 and HGF expression in reserpine-injuried gastric mucosa in the rat, European journal of pharmacology, 518(2-3), 221-226.
  • Dong, M., Yang, G., Liu, H., Liu, X., Lin, S., Sun, D., & Wang, Y. (2014). Aged black garlic extract inhibits HT29 colon cancer cell growth via the PI3K/Akt signaling pathway, Biomedical Reports, 2(2), 250-254.
  • El-Ashmawy, N. E., Khedr, E. G., El-Bahrawy, H. A., & Selim, H. M. (2016). Gastroprotective effect of garlic in indomethacin induced gastric ulcer in rats, Nutrition (Burbank, Los Angeles County, Calif.), 32(7-8), 849–854. https://doi.org/10.1016/j.nut.2016.01.010
  • Jeong, Y.; Ryu, J.; Shin, J.; Kang, M.; Kang, J.; Han, J.; Kang, D. (2016). Comparison of anti-oxidant and anti-inflammatory effects between fresh and aged black garlic extracts, Molecules, 21, 430.
  • Kim, D.; Kang, M.J.; Hong, S.S.; Choi, Y.-H.; Shin, J.H. (2017). Anti inflammatory effects of functionally active compounds isolated from aged black garlic, Phytotherapy research, 31, 53–61.
  • Lakshmi, V., Singh, N., Shrivastva, S., Mishra, S. K., Dharmani, P., Mishra, V., & Palit, G. (2010). Gedunin and photogedunin of Xylocarpus granatum show significant anti-secretory effects and protect the gastric mucosa of peptic ulcer in rats, Phytomedicine: international journal of phytotherapyandphytopharmacology.17(8-9),569–574. doi.org/10.1016/j.phymed.2009.10.016
  • Lee, T.W.; Bae, E.; Kim, J.H.; Jang, H.N.; Cho, H.S.; Chang, S.H.; Park, D.J. (2019). The aqueous extract of aged black garlic ameliorates colistin-induced acute kidney injury in rats, Renal failure, 41, 24–33.
  • Maldonado, P.D.; Barrera, D.; Medina-Campos, O.N.; Hernández-Pando, R.; Ibarra-Rubio, M.E.; Pedraza-Chaverri, J. (2003). Aged garlic extract attenuates gentamicin induced renal damage and oxidative stress in rats, Life sciences, 73, 2543–2556.
  • Memariani, Z., Sharifzadeh, M., Bozorgi, M., Hajimahmoodi, M., Farzaei, M. H., Gholami, M., Siavoshi, F., & Saniee, P. (2017). Protective effect of essential oil of Pistacia atlantica Desf. on peptic ulcer: role of α-pinene, Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan. 37(1), 57–63. https://doi.org/10.1016/s0254-6272(17)30027-4
  • Oloyede, H. O., Adaja, M. C., Ajiboye, T. O., & Salawu, M. O. (2015). Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats, Journal of integrative medicine, 13(2), 105–114. https://doi.org/10.1016/S2095-4964(15)60160-1
  • Sabiu, S., Garuba, T., Sunmonu, T., Ajani, E., Sulyman, A., Nurain, I., & Balogun, A. (2015). Indomethacin-induced gastric ulceration in rats: Protective roles of Spondias mombin and Ficus exasperata, Toxicology reports, 2, 261–267. https://doi.org/10.1016/j.toxrep.2015.01.002
  • Suleyman H, Albayrak A, Bilici M, Cadirci E, Halici Z. (2010). Different mechanisms in formation and prevention of indomethacin-induced gastric ulcers, Inflammation, 33: 224–34.
  • Tak, H.-M.; Kang, M.-J.; Kim, K.M.; Kang, D.; Han, S.; Shin, J.-H. (2014). Anti-inflammatory activities of fermented black garlic, Journal of the Korean Society of Food Science and Nutrition, 43, 1527–1534.
  • Wang, X., Jiao, F., Wang, Q. W., Wang, J., Yang, K., Hu, R. R., Wang, Y. S. (2012). Aged black garlic extract induces inhibition of gastric cancer cell growth in vitro and in vivo, Molecular Medicine Reports, 5(1), 66-72.

Investigation of the Protective Effects of Black Garlic Extract an Experimental Gastric Ulcer Model in Rats

Yıl 2024, Cilt: 17 Sayı: 1, 62 - 67, 31.03.2024
https://doi.org/10.30607/kvj.1416456

Öz

This study aimed to evaluate the effects of black garlic extract in preventing gastric ulcers. For this purpose, twenty-eight 2-3 month-old Spraque dawley rats were randomly divided into four groups: the Control group (CG), Ulcer group (UG), Ulcer + pantoprazole treatment group (PTG), and Ulcer + Black garlic group (BGG). Stomach ulcers were induced by administering indomethacin at a dose of 25 mg.kg-1 to all groups except the control group. Then, pantoprazole (20 mg.kg-1) and black garlic extract (275 mg. rat) were administered to the treatment groups. After the study, stomach samples were collected and macroscopic (ulcer scoring and ulcerative area mm2) and histopathology examination (HE) were performed. In biochemical analysis, MDA (pg.ml-1), SOD (ng.ml-1) and CAT (ng.ml-1) levels were determined in the tissue. According to the macroscopic results obtained; Statistically significant changes were found between BGG and UG (p<0.0001). PTG showed better effects than all groups. In histopathology; no lesion was found on CG. There was a similarity between PTG and CG. While there were intense bleeding and ulcer foci in UG, only limited focal ulcers were found in BGG. MDA, the highest values were seen in UG. There was no statistical significance between PTG and BCG. The lowest SOD activity was in UG, and there was a similarity in PTG and BGG. The highest CAT activity was determined in CG and the lowest in UG. In conclusion; black garlic extract applied in a single and high dose (5% w.v-1, 275 mg. rat) showed partial protective activity against stomach ulcers.

Etik Beyan

Aydin Adnan Menderes University Animal Experiments Local Ethics Committee (Approval No: 64583101/2023/156)

Destekleyen Kurum

Self-funded.

Kaynakça

  • Ahmed, T., & Wang, C. K. (2021). Black Garlic and Its Bioactive Compounds on Human Health Diseases: A Review.Molecules (Basel, Switzerland), 26(16),5028. doi.org/10.3390/molecules26165028
  • Badr, G. M., & Al-Mulhim, J.A. (2014). The protective effect of aged garlic extract on nonsteroidal anti-inflammatory drug-induced gastric inflammations in male albino rats, Evidence-based complementary and alternative medicine: eCAM, 759642. https://doi.org/10.1155/2014/759642
  • Boyacioglu, M., Kum, C., Sekkin, S., Yalinkilinc, H. S., Avci, H., Epikmen, E. T., & Karademir, U. (2016). The effects of lycopene on DNA damage and oxidative stress on indomethacin-induced gastric ulcer in rats, Clinical nutrition (Edinburgh, Scotland), 35(2), 428–435. https://doi.org/10.1016/j.clnu.2015.03.006
  • Boyacıoglu, M. (2019). Protective Effects of Silymarin on Indomethacin-Induced Gastric Ulcer in Rats, Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 16(2), 109-114.
  • Cantarella, G., Martinez, G., Cutuli, V. M., Loreto, C., D'Alcamo, M., Prato, A., Clementi, G. (2005). Adrenomedullin modulates COX-2 and HGF expression in reserpine-injuried gastric mucosa in the rat, European journal of pharmacology, 518(2-3), 221-226.
  • Dong, M., Yang, G., Liu, H., Liu, X., Lin, S., Sun, D., & Wang, Y. (2014). Aged black garlic extract inhibits HT29 colon cancer cell growth via the PI3K/Akt signaling pathway, Biomedical Reports, 2(2), 250-254.
  • El-Ashmawy, N. E., Khedr, E. G., El-Bahrawy, H. A., & Selim, H. M. (2016). Gastroprotective effect of garlic in indomethacin induced gastric ulcer in rats, Nutrition (Burbank, Los Angeles County, Calif.), 32(7-8), 849–854. https://doi.org/10.1016/j.nut.2016.01.010
  • Jeong, Y.; Ryu, J.; Shin, J.; Kang, M.; Kang, J.; Han, J.; Kang, D. (2016). Comparison of anti-oxidant and anti-inflammatory effects between fresh and aged black garlic extracts, Molecules, 21, 430.
  • Kim, D.; Kang, M.J.; Hong, S.S.; Choi, Y.-H.; Shin, J.H. (2017). Anti inflammatory effects of functionally active compounds isolated from aged black garlic, Phytotherapy research, 31, 53–61.
  • Lakshmi, V., Singh, N., Shrivastva, S., Mishra, S. K., Dharmani, P., Mishra, V., & Palit, G. (2010). Gedunin and photogedunin of Xylocarpus granatum show significant anti-secretory effects and protect the gastric mucosa of peptic ulcer in rats, Phytomedicine: international journal of phytotherapyandphytopharmacology.17(8-9),569–574. doi.org/10.1016/j.phymed.2009.10.016
  • Lee, T.W.; Bae, E.; Kim, J.H.; Jang, H.N.; Cho, H.S.; Chang, S.H.; Park, D.J. (2019). The aqueous extract of aged black garlic ameliorates colistin-induced acute kidney injury in rats, Renal failure, 41, 24–33.
  • Maldonado, P.D.; Barrera, D.; Medina-Campos, O.N.; Hernández-Pando, R.; Ibarra-Rubio, M.E.; Pedraza-Chaverri, J. (2003). Aged garlic extract attenuates gentamicin induced renal damage and oxidative stress in rats, Life sciences, 73, 2543–2556.
  • Memariani, Z., Sharifzadeh, M., Bozorgi, M., Hajimahmoodi, M., Farzaei, M. H., Gholami, M., Siavoshi, F., & Saniee, P. (2017). Protective effect of essential oil of Pistacia atlantica Desf. on peptic ulcer: role of α-pinene, Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan. 37(1), 57–63. https://doi.org/10.1016/s0254-6272(17)30027-4
  • Oloyede, H. O., Adaja, M. C., Ajiboye, T. O., & Salawu, M. O. (2015). Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats, Journal of integrative medicine, 13(2), 105–114. https://doi.org/10.1016/S2095-4964(15)60160-1
  • Sabiu, S., Garuba, T., Sunmonu, T., Ajani, E., Sulyman, A., Nurain, I., & Balogun, A. (2015). Indomethacin-induced gastric ulceration in rats: Protective roles of Spondias mombin and Ficus exasperata, Toxicology reports, 2, 261–267. https://doi.org/10.1016/j.toxrep.2015.01.002
  • Suleyman H, Albayrak A, Bilici M, Cadirci E, Halici Z. (2010). Different mechanisms in formation and prevention of indomethacin-induced gastric ulcers, Inflammation, 33: 224–34.
  • Tak, H.-M.; Kang, M.-J.; Kim, K.M.; Kang, D.; Han, S.; Shin, J.-H. (2014). Anti-inflammatory activities of fermented black garlic, Journal of the Korean Society of Food Science and Nutrition, 43, 1527–1534.
  • Wang, X., Jiao, F., Wang, Q. W., Wang, J., Yang, K., Hu, R. R., Wang, Y. S. (2012). Aged black garlic extract induces inhibition of gastric cancer cell growth in vitro and in vivo, Molecular Medicine Reports, 5(1), 66-72.
Toplam 18 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Farmakoloji
Bölüm ARAŞTIRMA MAKALESİ
Yazarlar

Serdar Aktaş 0000-0003-3243-8093

Erken Görünüm Tarihi 8 Mart 2024
Yayımlanma Tarihi 31 Mart 2024
Gönderilme Tarihi 8 Ocak 2024
Kabul Tarihi 5 Mart 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 17 Sayı: 1

Kaynak Göster

APA Aktaş, S. (2024). Investigation of the Protective Effects of Black Garlic Extract an Experimental Gastric Ulcer Model in Rats. Kocatepe Veterinary Journal, 17(1), 62-67. https://doi.org/10.30607/kvj.1416456
AMA Aktaş S. Investigation of the Protective Effects of Black Garlic Extract an Experimental Gastric Ulcer Model in Rats. kvj. Mart 2024;17(1):62-67. doi:10.30607/kvj.1416456
Chicago Aktaş, Serdar. “Investigation of the Protective Effects of Black Garlic Extract an Experimental Gastric Ulcer Model in Rats”. Kocatepe Veterinary Journal 17, sy. 1 (Mart 2024): 62-67. https://doi.org/10.30607/kvj.1416456.
EndNote Aktaş S (01 Mart 2024) Investigation of the Protective Effects of Black Garlic Extract an Experimental Gastric Ulcer Model in Rats. Kocatepe Veterinary Journal 17 1 62–67.
IEEE S. Aktaş, “Investigation of the Protective Effects of Black Garlic Extract an Experimental Gastric Ulcer Model in Rats”, kvj, c. 17, sy. 1, ss. 62–67, 2024, doi: 10.30607/kvj.1416456.
ISNAD Aktaş, Serdar. “Investigation of the Protective Effects of Black Garlic Extract an Experimental Gastric Ulcer Model in Rats”. Kocatepe Veterinary Journal 17/1 (Mart 2024), 62-67. https://doi.org/10.30607/kvj.1416456.
JAMA Aktaş S. Investigation of the Protective Effects of Black Garlic Extract an Experimental Gastric Ulcer Model in Rats. kvj. 2024;17:62–67.
MLA Aktaş, Serdar. “Investigation of the Protective Effects of Black Garlic Extract an Experimental Gastric Ulcer Model in Rats”. Kocatepe Veterinary Journal, c. 17, sy. 1, 2024, ss. 62-67, doi:10.30607/kvj.1416456.
Vancouver Aktaş S. Investigation of the Protective Effects of Black Garlic Extract an Experimental Gastric Ulcer Model in Rats. kvj. 2024;17(1):62-7.

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