Determination of Antimicrobial Activity of Cream Formulation Developed with Hibiscus rosa-sinensis Extract and Probiotic

Yıl 2023, Cilt: 13 Sayı: 2, 126 - 132, 22.12.2023

Ali Sağlam Meltem Aşan Özüsağlam İrem Çelik

https://doi.org/10.53518/mjavl.1327299

Öz

Medicinal plants are an indispensable source of new and effective pharmaceutical products. In the study, the biological activities of water extract obtained from the H. rosa-sinensis leaf was determined and its potential for use in the pharmaceutical and cosmetic industries was investigated. Disc diffusion assay and micro-dilution method against clinical origin test microorganisms were used to assess biological activity. The Extract showed a zone of inhibition on the tested bacteria and yeasts in the 6.85 mm to 10.74 mm range. MIC and MBC or MFC values of the Extract was determined as 6.25-12.50 µg/µL and 12.50-50 µg/µL. Then, the cream formulation containing H. rosa-sinensis leaf water extract and/or human milk originated probiotic candidate Limosilactobacillus fermentum MA-7 strain have been developed. The Extract and probiotic containing cream formulations showed variable antibacterial and antifungal effects on the tested clinical originated microorganisms. The highest inhibition zone diameters of Cream + Extract + Probiotic formulation group was obtained against Candida glabrata RSKK 04019 (9.09 mm) and Escherichia coli O157:H7 (9.04 mm). The results indicate that L. fermentum MA-7 and H. rosa-sinensis water extract, alternative to synthetic antimicrobials, may be used as a natural bioactive ingredient in daily personal care and clinical applications in the near future.

Anahtar Kelimeler

Camellia, Cream formulation, Extract, Natural additive, Probiotic

Kaynakça

• Aladeboyeje, O., and Şanlı, N. Ö. (2021). Fermented traditional probiotic beverages of Turkish origin: a concise review. International Journal of Life Sciences and Biotechnology, 4(3), 546-564. https://doi.org/10.38001/ijlsb.936982
• Al-Shammari, K. I., Batkowska, J., Gryzińska, M., Wlazło, Ł., Ossowski, M., & Nowakowicz-Dębek, B. (2022). The use of selected herbal preparations for the disinfection of Japanese quail hatching eggs, Poultry Science, 101(10), 102066. https://doi.org/10.1016/j.psj.2022.102066
• Al-Zoreky, N. S. (2009). Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels. International Journal of Food Microbiology, 134(3), 244-248. https://doi.org/10.1016/j.ijfoodmicro.2009.07.002
• Anil, K., & Ashatha, S. (2012). Review on Hibiscus rosa sinensis. International Journal of Research in Pharmaceutical and Biomedical Sciences, 3(2), 534-538. http://www.ijrpbsonline.com/files/13-3213.pdf
• Asan-Ozusaglam, M., & Celik, I. (2023). White pitahaya as a natural additive: potential usage in cosmetic industry. Foods and Raw materials, 11(1), 57-63. https://dx.doi.org/10.21603/2308-4057-2023-1-552
• Asan-Ozusaglam, M., & Gunyakti, A. (2019). Lactobacillus fermentum strains from human breast milk with probiotic properties and cholesterol-lowering effects. Food Science and Biotechnology, 28, 501-509. https://doi.org/10.1007/s10068-018-0494-y
• Baj, T., Biernasiuk, A., Wróbel, R., & Malm, A. (2020). Chemical composition and in vitro activity of Origanum vulgare L., Satureja hortensis L., Thymus serpyllum L. and Thymus vulgaris L. essential oils towards oral isolates of Candida albicans and Candida glabrata. Open Chemistry, 18(1), 108-118. https://doi.org/10.1515/chem- 2020-0011
• Bhaskar, A., & Nithya, V. (2012). Evaluation of the wound-healing activity of Hibiscus rosa sinensis L (Malvaceae) in Wistar albino rats. Indian Journal of Pharmacology, 44(6), 694. https://doi.org/10.4103%2F0253-7613.103252
• Calixto, J. B. (2019). The role of natural products in modern drug discovery. Anais da Academia Brasileira de Ciências, 91. https://doi.org/10.1590/0001-3765201920190105
• Harris-Tryon, T .A., & Grice, E. A. (2022). Microbiota and maintenance of skin barrier function. Science, 376(6596), 940-945. https://doi.org/10.1126/science.abo0693
• Haryani, Y., Halid, N. A., Guat, G. S., Nor-Khaizura, M. A. R., Hatta, A., Sabri, S., ... & Hasan, H. (2023). Characterization, molecular identification, and antimicrobial activity of lactic acid bacteria isolated from selected fermented foods and beverages in Malaysia. FEMS Microbiology Letters, 370, fnad023. https://doi.org/10.1093/femsle/fnad023
• Khristi, V., & Patel, V. H. (2016). Therapeutic potential of Hibiscus rosa sinensis: A review. International Journal of Nutrition and Dietetics, 4(2), 105-123. http://dx.doi.org/10.17654/ND004020105
• Kim, S. K., Guevarra, R. B., Kim, Y. T., Kwon, J., Kim, H., Cho, J. H., Kim, J. B., & Lee, J. H. (2019). Role of probiotics in human gut microbiome-associated diseases. Microbiol. Biotechnol, 29(9), 1335-1340. https://doi.org/10.4014/jmb.1906.06064
• Kowalska-Krochmal, B., & Dudek-Wicher, R. (2021). The minimum inhibitory concentration of antibiotics: Methods, interpretation, clinical relevance. Pathogens, 10(2), 165. https://doi.org/10.3390/pathogens10020165
• Lee J.Y., Kim Y., Kim J.I., Lee H.Y., Moon G.S. and Kang C.H. (2022). Improvements in Human Keratinocytes and Antimicrobial Effect Mediated by Cell-Free Supernatants Derived from Probiotics. Fermentation, 8(7), 332- 339. https://doi.org/10.3390/fermentation8070332
• Nakatsuji, T., Chen, T. H., Narala, S., Chun, K. A., Two, A. M., Yun, T., ... & Gallo, R. L. (2017). Antimicrobials from human skin commensal bacteria protect against Staphylococcus aureus and are deficient in atopic dermatitis. Science Translational Medicine, 9(378), eaah4680. https://doi.org/10.1126/scitranslmed.aah4680
• Palani Kumar, M. K., Halami, P. M., & Serva Peddha, M. (2021). Effect of Lactobacillus fermentum MCC2760- based probiotic curd on hypercholesterolemic C57BL6 mice. ACS Omega, 6(11): 7701-7710. https://doi.org/10.1021/acsomega.1c00045
• Pandey, A., Mazumder, A., & and Das, S. (2023). A critical evaluation of beneficial gut flora: Probiotics, prebiotics, postbiotics and synbiotics to treat constipation and haemorrhoids. Allelopathy Journal, 59(2), 187- 196.
• Patel, R., Patel, A., Vaghasiya, D., & Nagee, A. (2012). Antimicrobial evaluation of Hibiscus rosa-sinensis plant extracts against some pathogenic bacteria. Bulletin of Environmental and Scientific Research, 1(3-4), 14-17. http://www.besr.org.in/
• Pooja, K. A., Dudeja, S., Chauhan, R., Hemalata, S., Beniwal, V., Chhokar, V., & Kumar, A. (2016). Antimicrobial activity of ethno-medicinal plants against cariogenic pathogens. Journal of Medicinal Plants Studies, 4(3), 283-290. https://www.plantsjournal.com/archives/2016/vol4issue3/PartD/4-4-37-333.pdf
• Roudsari, M. R., Karimi, R., Sohrabvandi, S., & Mortazavian, A. M. (2015). Health effects of probiotics on the skin. Critical Reviews in Food Science and Nutrition, 55(9), 1219-1240. https://doi.org/10.1080/10408398.2012.680078
• Shandilya, S., & Pathak, V. (2020). Chemical constituents & pharmacological effects of Hibiscus rosa-sinensis (china rose)–a review. World Journal of Pharmaceutical Research, 10(1), 858-869. https://doi.org/10.20959/wjpr20211-19535
• Salmerón-Manzano E, Garrido-Cardenas JA, Manzano-Agugliaro F. Worldwide Research Trends on Medicinal Plants. Int J Environ Res Public Health. 2020 May 12;17(10):3376. https://doi.org/10.3390/ijerph17103376
• Schifano, E., Zinno, P., Guantario, B., Roselli, M., Marcoccia, S., Devirgiliis, C., & Uccelletti, D. (2019). The foodborne strain Lactobacillus fermentum MBC2 triggers pept-1-dependent pro-longevity effects in Caenorhabditis elegans. Microorganisms, 7(2), 45.
• Uçar, M. A., Derun, E. M., & Pişkin, M. B. (2023). Determination of usage potential of Hypericum perforatum, Hypericum capitatum, Centaurea cyanus extracts and creams in the cosmetic industry. Sigma, 41(3):443- 450. 10.14744/sigma.2023.00051
• Udo, I. J., Ben, M. G., Etuk, C. U., & Tiomthy, A. I. (2016). Phytochemical, proximate and antibacterial properties of Hibiscus rosa-sinensis L. Leaf. Journal of Medicinal Plants Studies, 4(5), 193-195.
• Woodhams, D. C., Rollins-Smith, L. A., Reinert, L. K., Lam, B. A., Harris, R. N., Briggs, C. J., & Bigler, L. (2020). Probiotics modulate a novel amphibian skin defense peptide that is antifungal and facilitates growth of antifungal bacteria. Microbial Ecology, 79, 192-202. https://doi.org/10.1007/s00248-019-01385-9