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Helicobacter pylori ile Enfekte Çocukların Yanak Epiteli Döküntü Hücrelerinde Mikronükleer ve Binükleer Hücre Sıklığının Değerlendirilmesi

Yıl 2023, Cilt: 7 Sayı: 1, 45 - 50, 28.04.2023
https://doi.org/10.29058/mjwbs.1205737

Öz

Amaç: Helicobacter pylori (H. pylori) gastroduodenal inflamasyon, ülser ve atrofik gastrite yol açan
bakteriyel bir patojendir. Mikronüklus (MN)’ lar hücrenin mitoz bölünmesi sırasında ortaya çıkan esas
çekirdeğe dahil olmayan oluşumlardır. Bu çalışmada, H. pylori ile mikronükleer ve binükleer hücre
arasındaki ilişkinin eksfolyatif sitoloji yöntemi ile değerlendirilmesi amaçlanmıştır.
Gereç ve Yöntemler: Bu çalışmada Zonguldak Bülent Ecevit Üniversitesi Uygulama ve Araştırma
Hastanesi Çocuk Gastroenteroloji polikliniğine yaşam kalitesini etkileyecek tarzda ciddi dispeptik
yakınmalarla başvuran ve endoskopik biyopsi yapılan hastalar yer aldı. Biyopsi örnekleri formalinde
fikse edildi ve parafin bloklardan hazırlanan kesitler Hematoksilen & Eozin (H&E) ile boyandı. H.
pylori varlığı açısından ışık mikroskobik olarak değerlendirildi. Biyopsi sonucu sadece H. pylori pozitif
örnekler çalışma grubu (n=30) hiçbir enfeksiyon etkeni saptanmayanlar ise kontrol grubu (n=30) olarak
alındı. Tüm hastalardan alınan yanak epitel hücreleri lamlara yayıldı, % 95’lik etil alkolde fikse edildi ve
Papanicolaou boyama yöntemine göre boyandı. Mikronükleer ve binükleer hücreler iyi boyanmış 1000
epitel hücrede sayıldı. İstatistiksel değerlendirme SPSS 18.0 (SPSS Inc., Chicago, IL, USA) programı
kullanıldı ve p<0.05 anlamlı olarak kabul edildi.
Bulgular: Çalışmamızda yer alan çocuklar 7 ile 15 yaşları arasında olup çalışma grubunun yaş ortalaması
11,87± 2,92, kontrol grubunun ise 11,63± 2.73’tür. H. pylori pozitif çocuklarda mikronükleer ve
binükleer hücreler kontrol grubuna göre anlamlı derecede yüksek bulundu (p<0.001).
Sonuç: Mikronükleer ve binükleer hücreler, H.pylori patogenezinde genotoksik hasarın ve düzensiz
sitoplazma bölünmesinin önemli olduğunu vurgulamıştır. Kullanılan yöntemin H. pylori ön tanısında kolay
uygulanabilen, az maliyetli invaziv olmayan tanı yöntemi olma yönünde aday olabileceği düşünülmekle
birlikte bu sonucun daha geniş örneklemle teyit edilmesine ihtiyaç bulunmaktadır

Destekleyen Kurum

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Proje Numarası

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Teşekkür

Füruzan Köktürk

Kaynakça

  • 1. Marshall BJ, Warren JR. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 1984;1:1311.
  • 2. Sharndama HC, Mba IE. Helicobacter pylori: An up-to-date overview on the virulence and pathogenesis mechanisms. Braz J Microbiol 2022;53(1):33-50.
  • 3. Tünger Ö. Helicobacter pylori infeksiyonları. Turkish Journal of Infection 2008;22 (2):107-115.
  • 4. Mărginean CO, Cotoi OS, Pitea AM, Mocanu S, Mărginean C. Assessment of the relationship between Helicobacter pylori infection, endoscopic appearance and histological changes of the gastric mucosa in children with gastritis (a single center experience). Rom J Morphol Embryol 2013;54(3 Suppl):709-715.
  • 5. Honar N, Minazadeh A, Shakibazad N, Haghighat M, Saki F, Javaherizadeh H. Diagnostic accuracy of urea breath test for Helicobacter pylori infection in children with dyspepsia in comparison to histopathology. Arq Gastroenterol 2016;53(2):108-112.
  • 6. Ustundag GH, Altuntas H, Soysal YD, Kokturk F. The effects of synbiotic “bifidobacterium lactis B94 plus inulin” addition on standard triple therapy of helicobacter pylori eradication in children. Can J Gastroenterol Hepatol 2017;2017:8130596.
  • 7. Güven B, Gülerman F, Kaçmaz B. Helicobacter pylori enfeksiyonu olan çocuklarda tanı testlerinin, klinik ve histopatolojik bulguların değerlendirilmesi. Van Tıp Derg 2019;26(3):298.
  • 8. Wang YK, Kuo FC, Liu CJ, Wu MC, Shih HY, Wang SS, Wu JY, Kuo CH, Huang YK, Wu DC. Diagnosis of Helicobacter pylori infection: Current options and developments. World J Gastroenterol 2015;21(40):11221-11235.
  • 9. Schmausser B, Mueller SO, Eck M, Möller M, Müller-Hermelink H, Stopper H: Helicobacter pylori induces DNA damage in vitro. Cancer Lett 2000;152(2):145-149.
  • 10. Kalisperati P, Spanou E, Pateras IS, Korkolopoulou P, Varvarigou A, Karavokyros I, Gorgoulis VG, Vlachoyiannopoulos PG, Sougioultzis S. Inflammation, DNA damage, helicobacter pylori and gastric tumorigenesis. Front Genet 2017;8:20.
  • 11. Kidane D. Molecular mechanisms of H. pylori-Induced DNA double-strand breaks. Int J Mol Sci 2018;19(10):2891
  • 12. Chmiela M, Karwowska Z, Gonciarz W, Allushi B, Stączek P. Host pathogen interactions in Helicobacter pylori related gastric cancer. World J Gastroenterol 2017;23(9):1521-1540.
  • 13. Oral D, Yirün A, Erkekoğlu P. Epigenetic and genetic changes caused by Helicobacter pylori and their roles in gastric carcinogenesis. J Fac Pharm Ankara 2019:43(3):285-308.
  • 14. Demirel S, Zamani AG. Mikronükleus tekniği ve kullanım alanları. Genel Tıp Derg 2002;12(3):123.
  • 15. Şekeroğlu V, Atlı Şekeroğlu Z. Genotoksik hasarın belirlenmesinde mikronükleus testi. Turk Hij Den Biyol Derg 2011;68(4):241.
  • 16. Safi Oz Z. Micronucleus scoring: An available approach in the evaluation of genomic damage in exfoliative cervicovaginal cells. Ann Cytol Pathol 2020:5(1):064-067.
  • 17. Lu Y, Liu Y, Yang C. Evaluating in vitro DNA damage using comet assay. J Vis Exp 2017;(128):56450.
  • 18. Bloching M, Hofmann A, LautenschlaÈ ger CL, Berghaus A, Grummt T. Exfoliative cytology of normal buccal mucosa to predict the relative risk of cancer in the upper aerodigestive tract using the MN-assay. Oral Oncol 2000;36(6):550-555.
  • 19. Cohen J. Statistical Power Analysis for the Behavioral Sciences, Revised ed. 1977.
  • 20. Tolbert PE, Shy CM, Allen JW. Micronuclei and other nuclear anomalies in buccal smears: Methods development. Mutat Res 1992;271(1):69-77.
  • 21. Qh Z, Rq L. Helicobacter pylori in the oral cavity and gastric mucosa: A meta-analysis. J Oral Pathol Med 2011;40(4):317- 324.
  • 22. Guevara B, Cogdill AG. Helicobacter pylori: A review of current diagnostic and management strategies. Dig Dis Sci 2020;65(7):1917-1931.
  • 23. Mommersteeg MC, Yu J, Peppelenbosch MP, Fuhler GM. Genetic host factors in Helicobacter pylori-induced carcinogenesis: Emerging new paradigms. Biochim Biophys Acta Rev Cancer 2018;1869(1):42-52.
  • 24. Suárez S, Sueiro RA, Araujo M, Pardo F, Menéndez MD, Pardiñas MC, Alvarez A. Increased frequency of micronuclei in peripheral blood lymphocytes of subjects infected with Helicobacter pylori. Mutat Res 2007;626(1-2):162-170.
  • 25. Thomas P, Hecker J, Faunt J, Fenech M. Buccal micronucleus cytome biomarkers may be associated with Alzheimer’s disease. Mutagenesis 2007;22(6):371-379.
  • 26. Safi Oz Z. The Interaction between Human papillomavirus proteins and cytoskeletal filaments. In: Davy Vanden Broeck, (editor). Human Papillomavirus and Related Diseases - From Bench to Bedside - Research aspects. Intech Publisher, 2012: 291.

Evaluation of Micronucleer and Binucleer Cells Frequencies in Buccal Epithelial Cells of Children Infected with Helicobacter pylori

Yıl 2023, Cilt: 7 Sayı: 1, 45 - 50, 28.04.2023
https://doi.org/10.29058/mjwbs.1205737

Öz

Aim: Helicobacter pylori (H. pylori) is a bacterial pathogen that causes gastroduodenal inflammation,
ulcer and atrophic gastritis. Micronuclei (MNs) are formations that are not included in the main nucleus
that emerge during the mitosis of the cell. In this study, it was aimed to evaluate the relationship between
H. pylori and micronuclear and binuclear cells using exfoliative cytology method.
Material and Methods: In this study, patients who applied to the Pediatric Gastroenterology outpatient clinic of Zonguldak Bülent Ecevit
University Practice and Research Hospital with severe dyspeptic complaints that would affect their quality of life and underwent endoscopic
biopsy were included. Biopsy samples were fixed in formalin and sections prepared from paraffin blocks were stained with Hematoxylin &
Eosin (H&E). It was evaluated light microscopically for the presence of H. pylori. Only H. pylori positive specimens as a result of biopsy were
taken as the study group (n=30), and those with no infectious agents were taken as the control group (n=30). Buccal epithelial cells from all
patients were spread on slides, fixed in 95% ethanol and stained according to the Papanicolaou technique. Micronuclear and binuclear cells
were counted in 1000 well-stained epithelial cells. Statistical evaluation SPSS 18.0 (SPSS Inc., Chicago, IL, USA) program was used and
p<0.05 was considered significant.
Results: The children in our study were between the ages of 7 and 15, and the mean age of the study group was 11.87±2.92, and the control
group was 11.63±2.73. Micronuclear and binuclear cells were found to be significantly higher in H. pylori positive children compared to the
control group (p<0.001).
Conclusion: Micronuclear and binuclear cells emphasized that genotoxic damage and irregular cytoplasm division are important in the
pathogenesis of H.pylori. Although it is thought that the method used may be a candidate to be an easy-to-apply, low-cost non-invasive
diagnostic method in the preliminary diagnosis of H. pylori, this result needs to be confirmed with a larger sample.

Proje Numarası

-

Kaynakça

  • 1. Marshall BJ, Warren JR. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 1984;1:1311.
  • 2. Sharndama HC, Mba IE. Helicobacter pylori: An up-to-date overview on the virulence and pathogenesis mechanisms. Braz J Microbiol 2022;53(1):33-50.
  • 3. Tünger Ö. Helicobacter pylori infeksiyonları. Turkish Journal of Infection 2008;22 (2):107-115.
  • 4. Mărginean CO, Cotoi OS, Pitea AM, Mocanu S, Mărginean C. Assessment of the relationship between Helicobacter pylori infection, endoscopic appearance and histological changes of the gastric mucosa in children with gastritis (a single center experience). Rom J Morphol Embryol 2013;54(3 Suppl):709-715.
  • 5. Honar N, Minazadeh A, Shakibazad N, Haghighat M, Saki F, Javaherizadeh H. Diagnostic accuracy of urea breath test for Helicobacter pylori infection in children with dyspepsia in comparison to histopathology. Arq Gastroenterol 2016;53(2):108-112.
  • 6. Ustundag GH, Altuntas H, Soysal YD, Kokturk F. The effects of synbiotic “bifidobacterium lactis B94 plus inulin” addition on standard triple therapy of helicobacter pylori eradication in children. Can J Gastroenterol Hepatol 2017;2017:8130596.
  • 7. Güven B, Gülerman F, Kaçmaz B. Helicobacter pylori enfeksiyonu olan çocuklarda tanı testlerinin, klinik ve histopatolojik bulguların değerlendirilmesi. Van Tıp Derg 2019;26(3):298.
  • 8. Wang YK, Kuo FC, Liu CJ, Wu MC, Shih HY, Wang SS, Wu JY, Kuo CH, Huang YK, Wu DC. Diagnosis of Helicobacter pylori infection: Current options and developments. World J Gastroenterol 2015;21(40):11221-11235.
  • 9. Schmausser B, Mueller SO, Eck M, Möller M, Müller-Hermelink H, Stopper H: Helicobacter pylori induces DNA damage in vitro. Cancer Lett 2000;152(2):145-149.
  • 10. Kalisperati P, Spanou E, Pateras IS, Korkolopoulou P, Varvarigou A, Karavokyros I, Gorgoulis VG, Vlachoyiannopoulos PG, Sougioultzis S. Inflammation, DNA damage, helicobacter pylori and gastric tumorigenesis. Front Genet 2017;8:20.
  • 11. Kidane D. Molecular mechanisms of H. pylori-Induced DNA double-strand breaks. Int J Mol Sci 2018;19(10):2891
  • 12. Chmiela M, Karwowska Z, Gonciarz W, Allushi B, Stączek P. Host pathogen interactions in Helicobacter pylori related gastric cancer. World J Gastroenterol 2017;23(9):1521-1540.
  • 13. Oral D, Yirün A, Erkekoğlu P. Epigenetic and genetic changes caused by Helicobacter pylori and their roles in gastric carcinogenesis. J Fac Pharm Ankara 2019:43(3):285-308.
  • 14. Demirel S, Zamani AG. Mikronükleus tekniği ve kullanım alanları. Genel Tıp Derg 2002;12(3):123.
  • 15. Şekeroğlu V, Atlı Şekeroğlu Z. Genotoksik hasarın belirlenmesinde mikronükleus testi. Turk Hij Den Biyol Derg 2011;68(4):241.
  • 16. Safi Oz Z. Micronucleus scoring: An available approach in the evaluation of genomic damage in exfoliative cervicovaginal cells. Ann Cytol Pathol 2020:5(1):064-067.
  • 17. Lu Y, Liu Y, Yang C. Evaluating in vitro DNA damage using comet assay. J Vis Exp 2017;(128):56450.
  • 18. Bloching M, Hofmann A, LautenschlaÈ ger CL, Berghaus A, Grummt T. Exfoliative cytology of normal buccal mucosa to predict the relative risk of cancer in the upper aerodigestive tract using the MN-assay. Oral Oncol 2000;36(6):550-555.
  • 19. Cohen J. Statistical Power Analysis for the Behavioral Sciences, Revised ed. 1977.
  • 20. Tolbert PE, Shy CM, Allen JW. Micronuclei and other nuclear anomalies in buccal smears: Methods development. Mutat Res 1992;271(1):69-77.
  • 21. Qh Z, Rq L. Helicobacter pylori in the oral cavity and gastric mucosa: A meta-analysis. J Oral Pathol Med 2011;40(4):317- 324.
  • 22. Guevara B, Cogdill AG. Helicobacter pylori: A review of current diagnostic and management strategies. Dig Dis Sci 2020;65(7):1917-1931.
  • 23. Mommersteeg MC, Yu J, Peppelenbosch MP, Fuhler GM. Genetic host factors in Helicobacter pylori-induced carcinogenesis: Emerging new paradigms. Biochim Biophys Acta Rev Cancer 2018;1869(1):42-52.
  • 24. Suárez S, Sueiro RA, Araujo M, Pardo F, Menéndez MD, Pardiñas MC, Alvarez A. Increased frequency of micronuclei in peripheral blood lymphocytes of subjects infected with Helicobacter pylori. Mutat Res 2007;626(1-2):162-170.
  • 25. Thomas P, Hecker J, Faunt J, Fenech M. Buccal micronucleus cytome biomarkers may be associated with Alzheimer’s disease. Mutagenesis 2007;22(6):371-379.
  • 26. Safi Oz Z. The Interaction between Human papillomavirus proteins and cytoskeletal filaments. In: Davy Vanden Broeck, (editor). Human Papillomavirus and Related Diseases - From Bench to Bedside - Research aspects. Intech Publisher, 2012: 291.
Toplam 26 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Sağlık Kurumları Yönetimi
Bölüm Araştırma Makalesi
Yazarlar

Zehra Safi Öz 0000-0003-1359-5026

Gonca Handan Şahan Üstündağ 0000-0003-3027-833X

Halime Topuz Altuntaş 0000-0002-0696-8173

Nilüfer Onak Kandemir 0000-0001-9035-4475

Proje Numarası -
Yayımlanma Tarihi 28 Nisan 2023
Kabul Tarihi 20 Ocak 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 7 Sayı: 1

Kaynak Göster

Vancouver Safi Öz Z, Şahan Üstündağ GH, Topuz Altuntaş H, Onak Kandemir N. Helicobacter pylori ile Enfekte Çocukların Yanak Epiteli Döküntü Hücrelerinde Mikronükleer ve Binükleer Hücre Sıklığının Değerlendirilmesi. Med J West Black Sea. 2023;7(1):45-50.

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