Laurus nobilis Esansiyel Yağının Sıçanlarda İndometasin Kaynaklı Mide Ülserinin Azaltılmasında Potansiyel Rolü

Yıl 2024, Cilt: 9 Sayı: 1, 89 - 96, 11.03.2024

Hilal Üstündağ Necip Gökhan Taş Nezahat Kurt Gizem Issin Ferdane Danışman Kalındemirtaş Selcan Karakuş

https://doi.org/10.26453/otjhs.1417755

Öz

Amaç: Bu çalışmanın amacı, sıçanlarda indometasin (INDO) ile indüklenen gastrik ülserlere karşı Laurus nobilis yaprakları uçucu yağının (LANO) gastroprotektif etkilerini araştırmaktır.
Materyal ve Metot: Bu çalışmada, INDO ile indüklenen gastrik ülser modeli kullanıldı. 30 adet Sprague-Dawley sıçan beş gruba ayrıldı (n=6): Kontrol, LANO, INDO, famotidin (FAM) ile INDO, LANO ile INDO. INDO (25 mg/kg) ülserleri indüklerken, LANO ve FAM oral gavaj yoluyla sırasıyla 200 mg/kg ve 40 mg/kg olarak uygulandı. Gastrik dokular ülserasyon açısından histopatolojik incelemeye tabi tutuldu ve biyokimyasal analizlerle toplam oksidan durum (TOS), toplam antioksidan durum (TAS), oksidatif stres indeksi (OSI) ve nitrik oksit (NO) seviyeleri ölçüldü.
Bulgular: İndometasin grubuyla karşılaştırıldığında, LANO ile tedavi gastrik ülser odaklarının sayısını önemli ölçüde azalttı. Biyokimyasal olarak, LANO uygulaması TOS ve OSI seviyelerini düşürdü ve TAS seviyesini korudu, bu da oksidatif stresin azaldığını göstermektedir. Ayrıca, LANO'nun NO seviyelerini stabilize ettiği görüldü. Bu biyokimyasal bulgular histopatolojik inceleme ile desteklendi.
Sonuç: Çalışmanın sonuçları, LANO'nun INDO kaynaklı mide hasarına karşı korunmada faydalı olabileceğini göstermektedir. LANO'nun oksidatif stres belirteçleri ve NO seviyelerinde gözlenen modülasyonu, mide ülserlerinin yönetiminde potansiyel rolünü ortaya koymaktadır.

Anahtar Kelimeler

İndometasin, Laurus nobilis, mide ülseri, nitrik oksit, oksidatif stres

Potential Role of Laurus nobilis Essential Oil in Reducing Indomethacin-Induced Gastric Ulcer in Rats

Öz

Objective: This study aimed to investigate the gastroprotective effects of Laurus nobilis leaves essential oil (LANO) against indomethacin (INDO)-induced gastric ulcers in rats.
Materials and Methods: In this study, an indomethacin-induced gastric ulcer model was employed. 30 Sprague-Dawley rats were divided into five groups (n=6): Control, LANO, INDO, INDO with famotidine (FAM), and INDO with LANO. Indomethacin (25 mg/kg) induced ulcers, while LANO and FAM were administered by oral gavage at 200 mg/kg and 40 mg/kg, respectively. Gastric tissues underwent histopathological examination for ulceration, and biochemical assays measured total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), and nitric oxide (NO) levels.
Results: Compared to the INDO group, treatment with LANO significantly decreased the number of gastric ulcer foci. Biochemically, LANO moderated TOS and OSI levels and preserved TAS, indicating reduced oxidative stress. Additionally, LANO appeared to stabilize NO levels. These biochemical findings were corroborated by histopathological examination.
Conclusions: The study's results indicate that LANO may be beneficial in protecting against NSAID-induced gastric damage. LANO's observed modulation of oxidative stress markers and NO levels suggests its potential role in managing gastric ulcers.

Anahtar Kelimeler

Gastric ulcer, indomethacin, Laurus nobilis, nitric oxide, oxidative stress

Kaynakça

• 1. Srivastav Y, Kumar V, Srivastava Y, Kumar M. Peptic Ulcer Disease (PUD), Diagnosis, and Current Medication-Based Management Options: Schematic Overview. Journal of Advances in Medical and Pharmaceutical Sciences. 2023;25(11), 14-27. doi:10.9734/jamps/2023/v25i11651
• 2. Malfertheiner P, Schulz C. Peptic ulcer: chapter closed?. Digestive Diseases. 2020;38(2):112-116. doi: 10.1080/10520295.2023.2221040
• 3. Duysak L, Gülaboğlu M, Yüksel TN, Halıcı Z, Canayakın D, Demir GM. Protective Effects of Ethanol Extract from Hippophae rhamnoides L. Leaves against Indomethacin-Induced Gastric Ulcer in Rats. Black Sea Journal of Health Science. 2023;6(4), 697-704. doi: 10.19127/bshealthscience.1336015
• 4. Paparella A, Nawade B, Shaltiel-Harpaz L, Ibdah M. A review of the botany, volatile composition, biochemical and molecular aspects, and traditional uses of Laurus nobilis. Plants. 2022;11(9):1209. doi:10.3390/plants11091209
• 5. Mohammed RR, Omer AK, Yener Z, Uyar A, Ahmed AK. Biomedical effects of Laurus nobilis L. leaf extract on vital organs in streptozotocin-induced diabetic rats: Experimental research. Annals of Medicine and Surgery. 2021;61:188-197. doi:10.1016/j.amsu.2020.11.051
• 6. Belasli A, Ben Miri Y, Aboudaou M, et al. Antifungal, antitoxigenic, and antioxidant activities of the essential oil from laurel (Laurus nobilis L.): Potential use as wheat preservative. Food Science & Nutrition. 2020;8(9):4717-4729. doi:10.1002/fsn3.1650
• 7. Al-Omran SA, Chinnikrishnaiah V, Ganesh VS, et al. Investigation of in vitro and in vivo Pursuits of Laurus nobilis Extract on Acetic Acid-induced Ulcerative Colitis in Wistar Rats. 2023;57(3s):644-651. doi:10.5530/ijper.57.3s.73
• 8. Koçer O, Türkmen M, Eren Y. Osmaniye Yöresinde Doğal Olarak Yetişen Defne (Laurus nobilis L.), Murt (Myrtus communis L.) Bitkilerinin Uçucu Yağ Oran ve Bileşenlerinin Belirlenmesi. Turkish Journal of Agriculture-Food Science and Technology. 2022;10(1):75-80. doi:10.24925/turjaf.v10i1.75-80.4974
• 9. Wong CP, Kobayashi Y, Yang J. Safety studies of LAURESH® a standardized Laurus nobilis leaf extract. Fundamental Toxicological Sciences. 2023;10(4):143-156. doi:10.2131/fts.10.143
• 10. Lee EH, Shin JH, Kim SS, Lee H, Yang SR, Seo SR. Laurus nobilis leaf extract controls inflammation by suppressing NLRP3 inflammasome activation. Journal of cellular physiology. 2019;234(5):6854-6864. doi:10.1002/jcp.27434
• 11. Toktay E, Yayla M, Sahin L, et al. The effects of dragon fruit (Hylocereus polyrhizus) extract on indomethacin‐induced gastric ulcer in rats. Journal of Food Biochemistry. 2022;46(9):e14274. doi:10.1111/jfbc.14274
• 12. Tometri SS, Ahmady M, Ariaii P, Soltani MS. Extraction and encapsulation of Laurus nobilis leaf extract with nano-liposome and its effect on oxidative, microbial, bacterial and sensory properties of minced beef. Journal of Food Measurement and Characterization. 2020;14:3333-3344. doi:10.1007/s11694-020-00578-y
• 13. Natale G, Lazzeri G, Blandizzi C, et al. Seriate histomorphometry of whole rat gastric: an accurate and reliable method for quantitative analysis of mucosal damage. Toxicology and applied pharmacology. 2001;174(1):17-26. doi:10.1006/taap.2001.9193
• 14. Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clinical biochemistry. 2004;37(2):112-119. doi:10.1016/j.clinbiochem.2003.10.014
• 15. Erel O. A new automated colorimetric method for measuring total oxidant status. Clinical biochemistry. 2005;38(12):1103-1111. doi:10.1016/j.clinbiochem.2005.08.008
• 16. Ray A, Gulati K, Henke P. Stress gastric ulcers and cytoprotective strategies: perspectives and trends. Current Pharmaceutical Design. 2020. 26(25):2982-2990.
• 17. Pineda-Peña EA, Chávez-Piña AE. Gaseous mediators (H2S, NO and CO) a new approach in gastrointestinal protection. Mexican Journal of Medical Research ICSA. 2023;11(21):39-47. doi:10.29057/mjmr.v11i21.9436
• 18. Tarnawski AS, Ahluwalia A. The critical role of growth factors in gastric ulcer healing: the cellular and molecular mechanisms and potential clinical implications. Cells. 2021;10(8):1964. doi:10.3390/cells10081964
• 19. Soundararajan L, Dharmarajan A, Samji P. Regulation of pleiotropic physiological roles of nitric oxide signalling. Cellular signalling. 2023;101:110496. doi:10.1016/j.cellsig.2022.110496
• 20. Lanas A. Role of nitric oxide in the gastrointestinal tract. Arthritis research & therapy. 2008;10(2):1-6. doi:10.1186/ar2465
• 21. Sánchez-Mendoza ME, López-Lorenzo Y, Cruz-Antonio L, et al. Gastroprotective effect of juanislamin on ethanol-induced gastric lesions in rats: Role of prostaglandins, nitric oxide and sulfhydryl groups in the mechanism of action. Molecules. 2020;25(9):2246. doi:10.3390/molecules25092246
• 22. Ulucan A, Etiopathogenesis of peptic ulcers and prostaglandin relationship. Van Medical Journal. 2020;27(2):238-245. doi:10.5505/vtd.2020.35744
• 23. Tarnawski AS, Ahluwalia A. Molecular mechanisms of epithelial regeneration and neovascularization during healing of gastric and esophageal ulcers. Current medicinal chemistry. 2012;19(1):16-27. doi:10.2174/092986712803414088
• 24. Liang TY, Deng RM, Li X, et al. The role of nitric oxide in peptic ulcer: a narrative review. Medical Gas Research. 2021;11(1):42. doi:10.4103/2045-9912.310059
• 25. Ugan RA, Un H. The protective roles of butein on indomethacin induced gastric ulcer in mice. The Eurasian Journal of Medicine. 2020;52(3):265. doi:10.5152/eurasianjmed.2020.20022
• 26. Stefanova G, Girova T, Gochev V, et al. Comparative study on the chemical composition of laurel (Laurus nobilis L.) leaves from Greece and Georgia and the antibacterial activity of their essential oil. Heliyon. 2020;6(12). doi:10.1016/j.heliyon.2020.e05491
• 27. Yazıcı M, Mazlum Y, Mehmet N, Çiğdem Ü, Türkmen M, Akaylı T. Effects of adding laurel (Laurus nobilis) essential oil to the diet of tilapia fish on growth and intestinal histology. Aquatic Sciences and Engineering. 2022;37(4):195-204. doi:10.26650/ASE20221101489
• 28. Pérez S, Taléns-Visconti R, Rius-Pérez S, Finamor I, Sastre J. Redox signaling in the gastrointestinal tract. Free Radical Biology and Medicine. 2017;104:75-103. doi:10.1016/j.freeradbiomed.2016.12.048
• 29. Al-Mijalli SH, Mrabti HN, Ouassou H, et al. Chemical Composition, Antioxidant, Anti-Diabetic, Anti-Acetylcholinesterase, Anti-Inflammatory, and Antimicrobial Properties of Arbutus unedo L. and Laurus nobilis L. Essential Oils. Life. 2022;12(11):1876. doi:10.3390/life12111876
• 30. Mssillou I, Agour A, El Ghouizi A, Hamamouch N, Lyoussi B, Derwich E. Chemical composition, antioxidant activity, and antifungal effects of essential oil from Laurus nobilis L. flowers growing in Morocco. Journal of Food Quality. 2020;1-8:8819311. doi:10.1155/2020/8819311