Influence of Different Dietary Lipids on the Activity of Metabolic Enzymes in Brown Trout (Salmo trutta)

Yıl 2014, Cilt: 14 Sayı: 1, - , 07.07.2014

Ali Atasever Murat Arslan Necdet Sirkecioglu Abdulkadir Bayir Deniz Ekinci

https://doi.org/10.4194/1303-2712-v14_1_04

Öz

The objective of this experiment was to test the effect of different dietary lipids in brown trout feeding on the activity of metabolic enzymes, such as hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD). Four iso-nitrogenous and iso-lipidic casein-gelatin based experimental diets with varying dietary sources were formulated. The sources of dietary lipid were menhaden oil (MO diet, rich in highly unsaturated fatty acids), hazelnut oil (HO diet, predominantly oleic acid), a blend of linseed oil and soybean oil (1:1) (LO+SO diet, linoleic and linolenic acids), and soybean lecithin (LE diet, phospholipids; mostly linoleic acid). Fish with an initial individual weight of ~1g were distributed randomly in three replicate 50-L glass aquaria with a total of 30 fish per tank. Each tank received one of the four diets referenced above for 6 weeks. In all cases, the activity of all metabolic enzymes significantly increased (P<0.05) in comparison to their activities in initial fish. All enzyme activities had their highest values in fish fed LO+SO and lowest in fish fed HO diet. It is very important to note that total polyunsaturated fatty acid (PUFA) content of the lipids is in the same order indicating that activity of these enzymes are elevated with increasing PUFA concentrations.

Anahtar Kelimeler

Carbonic anhydrase, hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, lipids, brown trout.

Influence of Different Dietary Lipids on the Activity of Metabolic Enzymes in Brown Trout (Salmo trutta)

Öz

Mevcut çalışmanın amacı farklı diyetsel yağ kaynaklarının kahverengi alabalıkta hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) gibi metabolik enzimler üzerine etkisini araştırmaktır. Tek farkları içerdikleri yağ kaynağı olan dört iso-nitrogenous ve iso-lipidik kazein-jelatin tabanlı yem formüle edilmiştir. Diyetsel yağ kaynakları olarak, ringa balığı yağı (MO diyeti, çoklu doymamış yağ asitleri bakımından zengin), fındık yağı (HO diyeti, başlıca oleik asit içeriyor), keten tohumu yağı/ soya yağı karışımı (1:1) (LO+SO diyeti, linoleik asit ve linolenik asit bakımından zengin) ve soya lesitini (LE diyeti, forfolipid yapısında ve çoğunlukla linoleik asit içeriyor) kullanılmıştır. Başlangıç ağırlıkları yaklaşık 1g olan balıklar, 50 litrelik cam akvaryumlara 30’ar adet, üç tekerrürlü olarak konulmuştur. Balıklar deneysel yemlerle 6 hafta boyunca beslenmişlerdir. Altı haftalık deneme besleme denemesi sonunda, bütün gruplarda metabolik enzim aktiviteleri başlangıç değerlerine göre önemli artış göstermiştir (P<0,05). Bütün enzimler en yüksek aktivitelerini LO+SO diyeti ile beslenen balıklarda gösterirken, en düşük aktivitelerini HO diyeti ile beslenen balıklarda göstermişlerdir. Çalışmamız, enzim aktivelerindeki artışların diyetsel yağ kaynaklarındaki çoklu doymamış yağ asitlerinin artışına paralel olarak gerçekleştiğini göstermiştir.

Anahtar Kelimeler

Carbonic anhydrase, hexokinase, lucose-6-phosphatedehydrogenase, 6-phosphogluconate dehydrogenase, yağlar, kahverengi alabalık.

Kaynakça

• Aksakal, E., Ekinci, D., Erdogan, O., Beydemir, S., Alim, Z. and Ceyhun, S.B. 2011. Increasing stocking density causes inhibition of metabolic-antioxidant enzymes and elevates mRNA levels of heat shock protein 70 in rainbow trout. Livestock Science. 141: 69-75. doi: 1016/j.livsci.2011.07.006
• Arslan, M., Sirkecioglu, N., Bayir, A., Arslan, H. and Aras, M. 20 The influence of substitution of dietary fish oil with different vegetable oils on performance and fatty acid composition of brown trout, Salmo trutta. Turkish Journal of Fisheries and Aquatic Sciences, 12: 575-583. doi: 10.4194/1303-2712-v12_3_04
• Arzel, J., Martinez, Lopez, F.X, Métailler, R., Stéphan, G., Viau, M., Gandemer, G. and Guillaume, J. 1994. Effect of dietary lipid on growth performance and body composition of brown trout (Salmo trutta) reared in sea water. Aquaculture, 123: 361-375. doi: 1016/0044-8486(94)90071-X
• Årnesen, P., Krogdahl, A. and Kristiansen, I.Ø. 1993. Lipogenic enzyme activities in liver of Atlantic salmon Salmo salar L. Comparative Biochemistry and Physiology B-Biochemistry and Molecular Biology, 105: 541–546. doi: 10.1016/0305-0491(93)90086-K
• Balaydin, H.T., Soyut, H., Ekinci, D., Goksu, S., Beydemir, S., Menzek, A. and Sahin, E. 2012. Synthesis and carbonic anhydrase inhibitory properties of novel bromophenols including natural products. Journal of Enzyme Inhibition and Medicinal Chemistry, 27: 43doi: 10.3109/14756366.2011.574131
• Barroso, J.B., Peragón, J., García-Salguero, L., De la Higuera, M. and Lupiáñez, J.A. 1999. Variations in the kinetic behaviour of the NADPH-production systems in different tissues of the trout when fed on an amino-acid-based diet at different frequencies. International Journal of Biochemistry and Cell Biology, 31: 277-2 doi: 1016/S13572725(98)00114-9
• Bayir, A., Sirkecioglu, N., Bayir, M., Arslan, M., Gunes, M., Haliloglu, H.I., Aras, M. and Arslan, H. 2011. Effects of different dietary lipid sources on the growth, survival and fatty acid composition of brown trout juveniles, Salmo trutta. Israeli Journal of Aquaculture-Bamidgeh, 622: 1-8.
• Bell, J.G., McEvoy, J., Tocher, D.R., McGhee, F., Campbell, P.J. and Sargent, J.R. 2001. Replacement of fish oil with rapeseed oil in diets of Atlantic salmon (Salmo salar) affects tissue lipid composition and hepatocyte fatty acid metabolism. Journal of Nutrition, 131: 1535–1543.
• Bell, J.G., Henderson, R.J., Tocher, D.R., Mc Ghee, F., Dick, J.R., Porter, A., Smullen, R.P. and Sargent, J.R. 200 Substituting fish oil with crude palm oil in the diet of Atlantic salmon (Salmo salar) affects muscle fatty acid composition and hepatic fatty acid metabolism. Journal of Nutrition, 132: 222–230. Bell, J.G., Tocher, D.R., Henderson, R.J, Dick, J.R. and Crampton, V.O. 200 Altered fatty acid compositions in Atlantic salmon (Salmo salar) fed diets containing linseed and rapeseed oils can be partially restored by a subsequent fish oil finishing diet. Journal of Nutrition, 133: 2793–2801.
• Beutler, E. 1971. Red cell metabolism: a manual of biochemical methods. Academic Press, London. 160 pp.
• Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254.
• Cakmak, R., Durdagi, S., Ekinci, D., Senturk, M. and Topal, G. 2011. Design, synthesis and biological evaluation of novel nitroaromatic compounds as potent glutathione reductase inhibitors. Bioorganic & Medicinal Chemistry Letters, 21: 5398–5402. doi: 1016/j.bmcl.2011.07.002
• Carvalho Cdos, S. and Fernandes, M.N. 2008. Effect of copper on liver key enzymes of anaerobic glucose metabolism from freshwater tropical fish Prochilodus lineatus. Comparative Biochemistry And Physiology A-Molecular & Integrative Physiology, 151: 437-42. doi: 1016/j.cbpa.2007.04.016
• Cavdar, H., Ekinci, D., Talaz, O., Saracoglu, N., Senturk, M. and Supuran, C.T. 2012. Alpha-Carbonic anhydrases are sulfatases with cyclic diol monosulfate esters. Journal of Enzyme Inhibition and Medicinal Chemistry,27:148–154. doi: 3109/14756366.2011.629198
• Ceyhun, S.B., Senturk, M., Ekinci, D., Erdogan, O., Ciltas, A. and Kocaman, M. 2010. Deltamethrin attenuates antioxidant defense system and induces the expression of heat shock protein 70 in rainbow trout. Comparative Biochemistry and Physiology CToxicology and Pharmacology, 152: 215–223. doi: 1016/j.cbpc.2010.04.008
• Ciftci, M., Beydemir, S. and Ekinci, D. 2008. Effects of some drugs on enzyme activity of glucose 6phosphate dehydrogenase from chicken erythrocytes in vitro. Asian Journal of Chemistry, 20: 2189-2196.
• Díez-Fernández, C., Sanz, N. and Cascales, M. 1996. Changes in glucose-6-phosphate dehydrogenase and malic enzyme gene expression in acute hepatic injury induced by thiochetamide. Biochemical Pharmacology, 51: 1159-1163. doi: 10.1016/00062952(96)00030-5
• Ekinci, D. and Beydemir, S. 2009. Evaluation of the impacts of antibiotic drugs on PON 1; a major bioscavenger against cardiovascular diseases. European Journal Pharmacology, 617: 84–89. doi: 1016/j.ejphar.2009.06.048
• Ekinci, D. and Senturk, M. 2013. Assesment of metal inhibition of antioxidant enzyme glutathione reductase from rainbow trout liver. Journal of Enzyme Inhibition and Medicinal Chemistry, 28: 11-15. doi: 3109/14756366.2011.615745
• Grisdale-Helland, B., Ruyter, B., Rosenlund, G., Obach, A., Helland, S.J., Gisvold, M., Standal, H. and Rİsjİ, C. 200 Influence of high contents of dietary soybean oil on growth, feed utilization, tissue fatty acid composition, heart histology and standard oxygen consumption of Atlantic salmon raised at two temperatures. Aquaculture, 207: 311–329. doi: 1016/S0044-8486(01)00743-8
• Hung, S.S.O. and Storebakken, T. 1994. Carbohydrate utilization by rainbow trout is affected by feeding strategy. Journal of Nutrition, 124: 223– 230.
• Kuo, W., Lin, J. and Tang, T.K. 2000. Human glucose-6phosphate dehydrogenase (G6PD) gene transforms nih 3t3 cells and induces tumors in nude mice. International Journal of Cancer, 85: 857-864. doi: 1002/(SICI)1097-0215(20000315)85:6<857::AIDIJC20>3.CO;2-L
• Lin, H., Romsos, D.R., Tack, P.I. and Leveille, G.A. 1977a. Influence of dietary lipid on lipogenic enzyme activities in coho salmon Oncorhynchus kisutch (Walbaum). Journal of Nutrition, 107: 846– 854.
• Lin, H., Romsos, D.R., Tack, P.I. and Leveille, G.A. 1977b. Effects of fasting and feeding various diets on hepatic lipogenic enzyme activities in coho salmon Oncorhynchus kisutch Walbaum. Journal of Nutrition, 107: 1477–1483.
• Iniesta, M.G., Cano, M.J. and Garrido-Pertierro, A. 1985.
• Properties and function of malate enzyme from Dicentrarchus labrax L. liver. Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, 80: 35– 39. doi: 10.1016/03050491(85)90418-3
• Menoyo, D., Lo´pez-Bote, C.J., Bautista, J.M. and Obach, A. 2003. Growth, digestibility and fatty acid utilization in large Atlantic salmon (Salmo salar) fed varying levels of n-3 and saturated fatty acids. Aquaculture, 225: 295–307. doi: 10.1016/S00448486(03)00297-7
• Menoyo, D., López-Bote, C.J., Obach, A. and Bautista, J.M. 200 Effect of dietary fish oil substitution with linseed oil on the performance, tissue fatty acid profile, metabolism, and oxidative stability of Atlantic salmon. Journal of Animal Science, 83: 2853-2862.
• Mercan, L., Sirkecioglu, N., Aksakal, E., Bayır, M., Bayır, A., Aras, M. and Ekinci, D. 2013. Goose fat, a promising nutrient for fish feeding, activates antioxidant enzymes in rainbow trout, Oncorhynchus mykiss. Environmental Toxicology and Pharmacology, 36: 964-971. doi: 1016/j.etap.2013.08.010
• NRC. 1993. Nutrient Requirements of Fish. The National Academy Press, Washington, DC, 128 pp.
• Regost, C., Arzel, J., Robin, J., Rosenlund, G. and Kaushik, S.J. 2003. Total replacement of fish oil by soybean or linseed oil with a return to fish oil in turbot (Psetta maxima) 1. Growth performance, flesh fatty acid profile, and lipid metabolism. Aquaculture, 217: 465– 4 doi: 10.1016/S0044-8486(02)00259-4
• Robey, R.B. and Hay, N. 2006 Mitochondrial hexokinases, novel mediators of the antiapoptotic effects of growth factors and Akt. Oncogene, 25: 4683–4696. doi: 1038/sj.onc.1209595
• Shimeno, S., Ming, D.C. and Takeda, M. 1993. Metabolic response to dietary carbohydrate to lipid ratios in Oreochromis niloticus. Nippon Suisan Gakkaishi, 59: 827–833.
• Thompson, K.D., Tatner, M.F. and Anderson, R.J. 1996. Effects of dietary (n-3) and (n-6) polyunsaturated fatty acid ratio on the immune response of Atlantic salmon, Salmo salar L. J. Aquaculture Nutrition, 2: 21–31. doi: 1111/j.1365-2095.1996.tb00004.x
• Torstensen, B. E., Lie Ø. and Frİyland L. 2000. Lipid metabolism and tissue composition in Atlantic salmon (Salmo salar L.) - Effects of capelin oil, palm oil, and oleic acid-enriched sunflower oil as dietary lipid sources. Lipids, 35: 653–664. doi: 10.1007/s11745000-0570-6
• TUIK (Turkey Statistics Organization) 2011 Agricultural Production Statistics for 2010, Ankara, Turkey.
• Walzem, R.L., Storebakken, T., Hung, S.S.O. and Hansen, R.J. 1991. Relationship between growth and selected liver enzyme activities of individual rainbow trout. Journal of Nutrition, 121: 1090– 1098.
• Wang, J-T., Liu, Y-J., Tian, L-X., Mai, K-S., Du, Z-Y., Wang, Y.and Yang, H-J. 2005. Effect of dietary lipid level on growth performance, lipid deposition, hepatic lipogenesis in juvenile cobia (Rachycentron canadum). Aquaculture, 249: 439-4 doi: 1016/j.aquaculture.2005.04.038
• Yildiz, M., Sener, E. and Timur M. 2006. Effect of Seasonal change and different commercial feeds on proximate composition of Sea Bream (Sparus aurata). Turkish Journal of Fisheries and Aquatic Sciences, 6: 99