Effect of Nitrogen Form on Trifoliate Orange (Poncirus trifoliata) and Sour Orange (Citrus aurantium) Plants Grown Under Saline Conditions

Louloudia Koulımboudı; Anna Papafılıppou; Maria Tzanoudakı; Christos Chatzıssavvıdıs

Effect of Nitrogen Form on Trifoliate Orange (Poncirus trifoliata) and Sour Orange (Citrus aurantium) Plants Grown Under Saline Conditions

Yıl 2014, Cilt: 1 Sayı: Özel Sayı-2, 1596 - 1605, 01.03.2014

Louloudia Koulımboudı Anna Papafılıppou Maria Tzanoudakı Christos Chatzıssavvıdıs

Öz

The effect of the form of nitrogen (N) on the vegetative growth and the chemical composition of trifoliate orange plants (Poncirus trifoliata) and sour orange plants (Citrus aurantium) irrigated with Hoagland nutrient solution with or without NaCl combined with three forms of N (nitrate, ammonium and their combination) was studied. At the end of the experiment, it was found that the trifoliate orange plants were more sensitive to salinity, since the weight of the fresh matter and the concentration of chlorophyll in the leaves were negatively affected. The highest values in the weight of the leaves and roots of the trifoliate orange plants were observed in the treatments with ammonium N, while the highest concentration of chlorophyll was observed in the treatments with a combination of nitrate and ammonium N under normal conditions. Furthermore, the highest values in the FW of the roots and shoots as well as in the chlorophyll units in the basal and top leaves were found in the sour orange plants which received a combination of nitrate and ammonium N under salinity conditions. Generally, N forms had different effects on the two genotypes in many cases. Finally, the inclusion of NaCl in the nutrient solution increased Na concentration in the leaves, the shoots and the roots of the two genotypes, whereas K concentration was reduced

Anahtar Kelimeler

nitrogen forms, salinity, sour orange, trifoliate orange, citrus

Kaynakça

Balal, R.M., Khan, M.M., Shahid, M.A., Mattson, N.S., Tahira, A., Muhammad, A., Garcia- Sanchez, F., Usman, G., Gimeno, V. and Zafar, I. 2012. Comparative studies on the physiobiochemical, enzymatic, and ionic modifications in salt-tolerant and salt- sensitive citrus rootstocks under NaCl stress. Journal of the American Society for
Horticultural Science 137: 86–95.
Bañuls, J., Serna, M.D., Legaz, M. and Primo-Millo, E. 1997. Growth and gas exchange parameters of citrus plants stressed with different salts. Journal of Plant Physiology 150: 194-199.
Behboudian, M.H., Torokfalvy, E. and Walker, R.R. 1986. Effect of salinity on ionic content, water relations parameters in some citrus scion-rootstock combinations. Scientia Horticulturae 28: 105-116. gas exchange
Ben-Hayyim, G. and Moore, G. 2007. Recent advances in breeding Citrus for drought and saline stress tolerance. In: M.A. Jenks et al. (eds.), Advances in Molecular Breeding Toward Drought and Salt Tolerant Crops, pp: 627–642.
Brumos, J., Colmenero-Flores, J.M., Conesa, A., Izquierdo, P., Sanchez, G., Iglesias, D.J., Lopez-Climent, M.F., Gomez-Cadenas, A. and Talon, M. 2009. Membrane transporters and carbon metabolism implicated in chloride homeostasis differentiate salt stress responses in tolerant and sensitive citrus rootstocks. Functional and Integrative Genomics 9: 293–309.
Castillo, C., Acuña, H., Zagal, E. and Inostroza, L. 2013. Phosphorus absorption and use efficiency by Lotus spp. under water stress conditions in two soils: a pot experiment. Chilean Journal of Agricultural Research 73: 31-40.
Cerda, A., Pardines, J., Botella, M.A. and Martinez, V. 1995. Effect of potassium on growth, water relations, and the inorganic organic solute contents for two maize grown under saline conditions. Journal of Plant Nutrition 18: 839-851.
Chatzissavvidis, C., Papadakis, I. and Therios, I. 2008. Effect of calcium on the ion status and growth performance of a citrus rootstock grown under NaCl stress. Soil Science and Plant Nutrition 54: 910-915.
Chatzissavvidis, C., Therios, I. and Antonopoulou, C. 2007. Effect of nitrogen source on olives growing in soils with high boron content. Australian Agriculture 47: 1491-1497. Experimental
Edwards, J.H. and Horton, B.D. 1982. Interaction of peach seedlings to NO3:NH4 ratios in nutrient solutions. Journal of the American Society for Horticultural Science 107: 142- 147.
Flowers, T. 2006. Plants and salinity –Special Issue. Preface. Journal of Experimental Botany vol. 57, no 5, p. iv.
Grieve, A.M. and Walker, R.R. 1983. Uptake and distribution of chloride, sodium and potassium ions in salt-treated citrus plants. Australian Journal of Agricultural Resources 34: 133–143.
Hafsi, C., Lakhdar, A., Rabhi, M., Debez, A., Abdelly, C. and Ouerghi, Z. 2007. Interactive effect of salinity and potassium availability on growth, water status, and ionic composition of Hordeum maritimum. Journal of Plant Nutrition and Soil Science 170: 469-473.
Kostopoulou, Z. and Therios, I. 2014. Growth and inorganic composition of “Nova” mandarin plants rootstocks in response to salinity and silicon. Acta Physiologiae Plantarum 36: 1363-1372.
Kostopoulou, Z., Therios, I. and Molassiotis, A. 2014. Resveratrol and its combination with α- tocopherol mediate salt adaptation in citrus seedlings. Plant Physiology and Biochemistry 78: 1-9.
Laϋchli, A. and Grattan, S.R. 2007. Plant growth and development under salinity stress. In: M.A. Jenks et al. (eds.), Advances In Molecular Breeding Toward Drought and Salt Tolerant Crops, pp: 1-32.
Lee, M.H., Cho, E.J., Wi, S.G., Bae, H., Kim, J.E., Cho, J.Y., Lee, S., Kim, J.H. and Chung, B.Y. 2013. Divergences in morphological changes and antioxidant responses in salt-tolerant and salt-sensitive rice seedlings after salt stress. Plant Physiology and Biochemistry 70: 325- 335.
Loreto, F., Centritto, M. and Chartzoulakis, K. 2003. Photosynthetic limitations in olive cultivars with different sensitivity to salt stress. Plant, Cell & Environment 26: 595-601.
Marschner, H.M. 1995. Mineral Nutrition of Higher Plants. Academic Press, p. 889.
Martinez Beltran, J. and Licona Manzur, C. 2005. Overview of salinity problems in the world and FAO strategies to address the problem. International Salinity Forum – Managing Saline Soils and Water: Science, Technology and Social Issues, p. 28.
Morais, M.C., Panuccio, M.R., Muscolo, A. and Freitas, H. 2012. Does salt stress increase the ability of the exotic legume Acacia longifolia to compete with native legumes in sand dune ecosystems? Environmental and Experimental Botany 82: 74-79.
Moya, J.L., Gomez-Cadenas, A., Primo-Millo, E. and Talon, M. 2003. Chloride absorption in salt- sensitive Carrizo citrange and salt-tolerant Cleopatra mandarin citrus rootstocks is linked to water use. Journal of Experimental Botany 54: 825 -833.
Munns, R. 2002. Comparative physiology of salt and water stress. Plant, Cell & Environment 25: 239-250.
Navarro, J.M., Pérez-Tornero, O. and Morte, A. 2014. Alleviation of salt stress in citrus seedlings mycorrhizal fungi depends on the rootstock arbuscular salt tolerance. Journal of Plant Physiology 171: 76-85.
Pitman, M. and Lauchli, A. 2002. Global impact of salinity and agricultural ecosystems. A. Lauchli, Environment-Plants-Molecules, Academic Netherlands, pp. 1–51. Salinity: Kluwer Publisher, Dordrecht,
Romero-Aranda, R., Moya, J.L., Tadeo, F.R., Legaz, F., Primo-Millo, E. and Talon, M. 1998. Physiological and anatomical disturbances induced by chloride salts in sensitive and tolerant citrus: beneficial and detrimental effects of cations. Plant, Cell & Environment 21: 1243-1253.
Roussos, P.A., Gasparatos, D., Kyriakou, C., Tsichli, K., Tsantili, E. and Haidouti, C. 2013. Growth, nutrient status and biochemical changes of sour orange plants subjected to sodium chloride stress. Communications in Soil Science and Plant Analysis 44: 805-816.
Ruiz, D., Martinez, V. and Cerda, A. 1997. Citrus response to salinity: growth and nutrient uptake. Tree Physiology 17: 141–150.
Saykhul, A., Chatzistathis, T., Chatzissavvidis, C., Koundouras, S. and Therios, I. 2013. Potassium utilization efficiency of three olive cultivars grown in a hydroponic system. Scientia Horticulturae 162: 55-62.
Shahbaz Akhtar, M., Oki, Y. and Adachi, T. 2010. Growth behaviour, nitrogen-form effects on phosphorus acquisition and phosphorus – zinc interactions in Brassica cultivars under phosphorus-stress Communications in Soil Science and Plant Analysis 41: 2022-2045. environment.
Simpson, C.R., Nelson, S.D., Melgar, J.C., Jifon, J., King, S.R., Schuster, G. and Volder, A. 2014. Growth response of grafted and ungrafted citrus trees to saline irrigation. Scientia Horticulturae 169: 199-205.
Speer, M. and Kaiser, W.M. 1991. Ion relations of symplastic and apoplastic space in leaves of Spinacea oleracea L. and Pisum sativum L. under salinity. Plant Physiology 97: 990-997.
Storey, R. and Walker, R.R. 1999. Citrus and salinity. Scientia Horticulturae 78: 39-81.
Syvertsen, J.P. and Garcia-Sanchez, F. 2014. Multiple abiotic stresses occurring with salinity stress in citrus. Environmental and Experimental Botany 103: 128-137.
Therios I. 1996. Inorganic Nutrition and Fertilisers, Dedousi publications, Thessaloniki, Greece, p. 75, 191-192
Thongbai, P., Hannam, R.J., Graham, R.D. and Webb, M.J. 1993. Interaction between zinc nutritional status of cereals and Rhizoctonia root rot severity. I. Field observation. Plant Soil 153: 207-214.
Torres de Claasen, M.E. and Wilcox, G.E. 1974. Effect of nitrogen form on growth and composition of tomato and pea tissue. Journal of the American Society for Horticultural Science 99: 171-174.
Trehan, S.P. 2009. Improving nutrient use efficiency by exploiting genetic diversity of potato. Potato Journal 36: 121-135.
Tsampardoukas B., Therios I. and Antonopoulou C. 2009 Effects of the ionic form of nitrogen on growth, physiological parameters of non-grafted olive plants (Olea europaea L.) of the "Kalamon" cultivar. Proceedings of the 23rd Conference of the Greek Society for Horticultural Science - Volume A: 185-188.
Walker, R.B. and Douglas, T.J. 1982. Effect of salinity on uptake and distribution of chloride, sodium, and potassium ions in citrus plants. Australian Journal of Agricultural Research 34: 145-153.
Zhu, J.K. 2001. Plant salt tolerance. Trends in Plant Science 6: 66-71.