Topraktaki Ağır Metallerin Absorbsiyonu Üzerine Bakteri İzolatlarının Etkisi

Yıl 2017, Cilt: 4 Sayı: 3, 355 - 365, 25.07.2017

Serdar Bilen Emrah Yıldırım

Öz

Bu çalışmada da toprakların ağır metal içerikleri üzerine çeşitli
bakteri izolatlarının etkileri araştırılmıştır. Araştırmada materyal olarak; 13
farklı bakteri izolatı ve çimento fabrikası baca dumanlarına maruz kalmış tarım
topraklarından alınan toprak örnekleri kullanılmıştır. Çalışma 2 aşamada
yürütülmüştür. I. Aşamada 8 adet ağır metal (Fe, Cu, Zn, Mn, Al, Ni, Cd ve Pb)
ihtiva eden Nutrient Broth besiyeri içerisine 13 farklı bakteri izolatları
ilave edilmiş, 2 saatlik inkübasyon sonrası bakteriler tarafından
gerçekleştirilen metal absorbsiyon kapasiteleri belirlenmiştir. II. Aşamada ise
toprak örneklerine I. Aşamadan elde edilen ve etkinliği belirlenen 5 farklı
bakteri (Staphylococcus cohnii, Sphingobium yanoikuyae, Vibrio hollisae, Xanthobacter flavus, Acinetobacter
lwoffi) izolatları topraklara aşılanmış, 6 haftalık inkübasyon sonrası
bakterilerin topraktaki metal absorbsiyon kapasiteleri belirlenmiştir. Araştırma sonuçlarına göre, S. cohnii izolatının toprakta en yüksek oranda Pb metalini, S. Yanoikuyae, V. Hollisae, X. flavus ve A. lwoffi izolatının en yüksek oranda Al metalini absorbe
ettikleri belirlenmiştir. Elde edilen sonuçlar ağır metal içeren
toprakların bioremediasyon çalışmalarında bazı bakterilerin kullanımının büyük
oranda katkı sağlayacağı sonucunu ortaya koymuştur.

 

Anahtar Kelimeler

Metal absorbsiyonu, bakteri metal absorbsiyon oranı, biyoremediasyon

Kaynakça

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