Determination of Antioxidant Activities in Melon (Cucumis melo L.) Seedlings Exposed to Arsenic Stress

Year 2019, Volume: 6 Issue: 2, 276 - 284, 22.04.2019

Yonca Surgun-acar

https://doi.org/10.30910/turkjans.557117

Abstract

Arsenic (As) is a toxic metalloid for all living
organisms. Uptake of As in plant tissues affects plant
metabolism and causes to several physiological and structural disorders. In this study, antioxidant profile of melon (Cucumis melo L.) seedlings exposed to different
arsenate [As(V)] treatments was investigated. Four seeds were sowed in
each magenta vessel including filter paper and following ten days after sowing,
melon seedlings were treated with Hoagland solution containing 0, 50, 100, 150 and 200 mg L^-1 disodium
hydrogen arsenate heptahydrate (Na₂HAsO₄.7H₂O) for 10 days. The experiment was set up in a plant growth cabinet as randomized plots
design with 3 replications. Superoxide
dismutase (SOD) (EC 1.15.1.1) and catalase (CAT) (EC 1.11.1.6) enzyme activities
which are the key enzymes of antioxidant system, total antioxidant and lipid
peroxidation levels, the contents of photosynthetic pigments (total chlorophyll
and carotenoids) and free proline were determined in leaf and root tissues of
melon seedlings. In leaves, high concentration of As(V) (200 mg L^-1)
treatment caused a significant decrease in total chlorophyll and carotenoid
content by 26% and 33%, respectively. SOD and CAT enzyme activities and total
antioxidant level increased in response to 100, 150 and 200 mg L^-1
As(V) treatments in roots. In leaves, while SOD and CAT activities increased at
50 and 100 mg L^-1 As(V) treatments, enzyme activities decreased as a
result of 100 and 150 mg L^-1 As(V) treatments compared with other
As(V) treatments. Lipid peroxidation, one of the indicators of oxidative
stress, increased in leaf and root tissues under As(V) stress when compared
control. In addition, all As(V) treatments caused an increase in free proline
content in both tissues significantly.

Keywords

Antioxidant system, arsenate, melon (Cucumis melo L.), oxidative stress.

Arsenik Stresine Maruz Kalan Kavun (Cucumis melo L.) Fidelerinde Antioksidan Aktivitelerinin Belirlenmesi

Abstract

Arsenik (As) tüm organizmalar için
toksik bir metaloiddir. Bitki dokularına As alımı bitki metabolizmasını
etkileyerek çeşitli fizyolojik ve yapısal bozukluklara neden olmaktadır. Bu çalışmada
farklı konsantrasyonlarda arsenat [As(V)] uygulamalarına maruz bırakılan kavun
(Cucumis melo L.) fidelerinin
antioksidan profili araştırılmıştır. Filtre kağıt içeren magenta kaplarına 4’er
adet tohum ekimi yapılmış ve ekimi takiben on gün sonra kavun fidelerine 10 gün
boyunca 0, 50, 100, 150 ve 200 mg L^-1 di-sodyum hidrojen arsenat
heptahidrat (Na₂HAsO₄.7H₂O) içeren
Hoagland solüsyonu uygulanmıştır. Deneme,
tesadüf parselleri deneme desenine göre 3 tekerrürlü olarak bitki büyütme
kabininde yürütülmüştür. Kavun
fidelerine ait yaprak ve kök dokularında antioksidan sistemin anahtar
enzimlerinden olan süperoksit dismutaz (SOD) (EC 1.15.1.1) ve katalaz (CAT) (EC
1.11.1.6) enzim aktiviteleri, toplam antioksidan ve lipid peroksidasyon
seviyeleri, fotosentetik pigment (toplam klorofil ve karotenoid) ve serbest
prolin içerikleri tespit edilmiştir. Arsenatın yüksek konsantrasyonda (200 mg L^-1)
yapılan uygulaması yapraklarda toplam klorofil ve karotenoid miktarının
sırasıyla %26 ve %33 azalmasına neden olmuştur. SOD ve CAT enzim aktiviteleri
ve toplam antioksidan seviyesi kök dokusunda 100, 150 ve 200 mg L^-1
As(V) uygulamaları sonucu artmıştır. Yapraklarda, SOD ve CAT enzim aktiviteleri
50 ve 100 mg L^-1 As(V) uygulamalarıyla artarken, 150 ve 200 mg L^-1
As(V) uygulamaları sonucu enzim aktiviteleri diğer As(V) uygulamalarına kıyasla
azalmıştır. Oksidatif hasarın indikatörlerinden biri olan lipid peroksidasyonu As(V)
stresi altında yaprak ve kök dokularında kontrole nazaran artmıştır. Ayrıca,
tüm As(V) uygulamaları her iki dokuda da serbest prolin miktarının anlamlı
olacak şekilde artmasına neden olmuştur.

Keywords

Antioksidan sistem, arsenat, kavun (Cucumis melo L.), oksidatif stres

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