Sıçanlarda oluşturulan cerrahi müdahalesiz yeni bir deneysel Asherman sendromu modeli

Öz

Amaç: Asherman sendromu(AS) uterin kaviteye travma sonucu kavitenin adezyonlarla kısmi ya da tam tıkanması durumudur. Klinik öncesi çalışmalarla yeni tedavi yöntemlerinin etkinliğini gösterebilmek için öncelikle AS modelinin hayvanda oluşturulmasına ihtiyaç vardır. Bu çalışmanın amacı, AS ve intrauterin adezyon modellemesinde kullanılmak üzere cerrahi uygulanmaksızın yeni ve etkili bir yöntem geliştirmek, bu şekilde araştırmacılara güvenlik ve uygulanabilirlik açısından daha etkin bir metot önerebilmektir.

Yöntem: On iki dişi Wistar Albino sıçan rastgele 2 gruba ayrıldı. Daha önceden hazırlanmış olan pembe renk (20 gauge) kateterle transvajinal yoldan hayvanların sol uterin hornularına ulaşıldı. Grup I’deki hayvanlara 0,2 ml serum fizyolojik uygulandı, Grup II’dekilere ise 0,2 ml Triklorasetik asit (TCA) uygulandı. Üç menstruel siklus beklendikten sonra hayvanların uterusları çıkarılıp Hematoksilen-Eozin ve Masson Trikrom boyamalar yapılarak histopatolojik olarak değerlendirildi. Yapılan Hematoksilen-Eozin boyalı kesitlerde inflamasyon dereceleri değerlendirilirken, Masson Trikrom boyalı kesitlerde fibrozis dereceleri değerlendirildi.

Bulgular: Grup 1’e ait uterus kesitleri normal histolojik görünüme sahipken, Grup 2’ye ait sol uterus kesitlerinde inflamasyon ve fibrozis oluştuğu gözlendi. Bu sonuçlar istatistiksel olarak anlamlıydı (p=0,002).

Sonuç: Sonuç olarak, önerdiğimiz cerrahi müdahalesiz AS modelleme yöntemi, literatürde önerilen yöntemler ile benzer şekilde hastalık oluşturmuş ve bu etki, yapılan histopatolojik incelemelerle de ortaya konmuştur. Bu şekilde, AS için doğru bir hastalık modellemesi gerçekleştiren cerrahisiz yeni bir yöntem önerilmiştir.

Anahtar Kelimeler

• Asherman sendromu,sıçan,jinatrezi,patoloji,hayvan modeli

A new nonsurgical experimental model for Asherman syndrome created in rats

Abstract

Aim: Asherman Syndrome (AS) is a partial or complete obstruction of the uterine cavity with adhesions as a result of trauma. In pre-clinical studies, to be able to show the effectiveness of new treatment methods, first of all, the AS model needs to be created in the animals. The aim of this study is to develop a new and effective nonsurgical method for using in AS and intrauterine adhesions modeling, and through this way, to propose a more effective method for researchers in terms of safety and feasibility.

Methods: Twelve female Wistar Albino rats were divided into two groups. It was reached to the left uterine horn transvaginally by using pre-prepared pink color (20 gauge) cannula. While 0.2 ml normal saline was applied to the animals in Group I (control group), 0.2 ml (Trichloroacetic acid) TCA was applied to the animals in Group II (experiment group).The right uterine horns of the animals were left without treatment. After three menstrual cycles, the animals were sacrificed and Hematoxylin-Eosin and Masson’s Trichrom staining were performed and evaluated histopathologically. Inflammation was evaluated in Hematoxylin-Eosin staining and fibrosis was evaluated in Masson’s Trichrom staining.

Results: Whereas the uterine sections of the Group I have normal histologic appearance, inflammation and fibrosis were found in the left uterine sections of the Group 2 by histopathological evaluation. Results were statistically significant (p=0.002).

Conclusion: The proposed nonsurgical AS modeling method created disease, and this was also revealed by histopathological examinations. Through this way, a new AS model is suggested without surgery, in which the disease is correctly created.

Keywords

• Asherman Syndrome,rat,gynatresia,pathology,animal model

Kaynakça

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