The investigation of antioxidant and anticancer effects of some importance medical plants

Yıl 2019, Cilt: 8 Sayı: 2, 435 - 442, 28.06.2019

Rabia Top Yavuz Erden , Suat Tekin

https://doi.org/10.17798/bitlisfen.485413

Cited By: 4

Öz

For
thousands of years, plants have been used for the treatment of many diseases.
In addition, in modern pharmacy many plant compounds have an important place in
the production of pharmaceutical raw materials or new medicines. The aim of
this study was to determine the biological activity of extracts obtained from
the fruits of the medical important bitter melon (Momordica charantia), pepino
(Solanum muricatum) and goldenberry (Physalis peruviana). The total polyphenol
content of the samples was measured spectrophotometrically using
Folin-Ciocaltute reactivity. The DPPH free radical scavenging effect of plant
samples was determined and antioxidant capacities were determined. In addition,
the cytotoxic effects of the samples on human over and breast cancer cell lines
(A2780 and MCF7, respectively) were determined using MTT method. As a result of
the study, it was determined that the highest total polyphenol content was in
the case of potency and the lowest content was in the case of pepino. All three
plant samples revealed the effect of free radical elimination of DPPH dependent
predation. Finally, it was determined that all three plant samples showed high
cytotoxic effect on both human over and breast cancer cell lines. Results show
us that in all three plant samples it has a significantly higher anticancer
effect than antioxidant effect. With the extensive studies to be carried out
after that, the mechanism of the effect can be enlightened and contribute to
new treatment approaches. 

Anahtar Kelimeler

Antioxidant, Cytotoxicity, Momordica charantia, Physalis peruviana, Solanum muricatum

Bazı önemli tıbbi bitkilerin antioksidan ve antikanser etkilerinin araştırılması

Öz

Tıbbi
açıdan önemli bitkiler binlerce yıldır birçok hastalığın tedavisi amacıyla
kullanılmaktadır. Buna ek olarak modern eczacılıkta birçok bitki bileşiği ilaç
ham maddesi veya yeni ilaçların yapımında önemli bir yere sahiptir. Bu çalışmada
tıbbi öneme sahip kudret narı (Momordica charantia), pepino (Solanum muricatum)
ve altın çilek (Physalis peruviana) bitkilerinin meyvelerinden elde edilen
özütlerin biyolojik etkinliklerinin ortaya konulması amaçlanmıştır. Bitkilerin
meyve özütleri %80’lik etanolde hazırlandıktan sonra toplam polifenol içeriği
Folin-Ciocaltute reaktifi kullanılarak spektrofotometrik olarak ölçüldü. Bitki
örneklerinin serbest radikal giderme etkisi DPPH serbest radikali kullanılarak
belirlendi. Özütlerin insan over ve meme kanseri hücre hatları (sıyasıyla A2780
ve MCF7) üzerine sitotoksik etkileri MTT yöntemi kullanılarak belirlendi. Bitki
örneklerindeki toplam polifenolik düzeyin en düşük 138,14±12,15 ve en yüksek
415,71±36,89 µg/gr gallik asit düzeyinde olduğu belirlendi. Bütün özütlerin
düşük seviyede antioksidan etki sergilediği saptandı. Son olarak over ve meme
kanseri hücre serilerine uygulanan özütlerin güçlü sitotoksik etki gösterdiği
belirlendi (p<0.05). Sonuçta her üç bitki örneğinin de antioksidan
etkilerinden ziyade yüksek antikanser etkiye sahip olduğunu bizlere
göstermektedir. Her üç örneğinde söz konusu kanser türleri üzerine etkilerini
aydınlatmak için ileriki çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

Antioksidan, Sitotoksisite, Momordica charantia, Physalis peruviana, Solanum muricatum

Kaynakça

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