Year 2017, Volume 38, Issue 4, Pages 52 - 59 2017-12-08

Determination of Phylogenetic Relationships of Five Species of Centaurea Genus by Using trnT-L-F cpDNA Region
Centaurea Cinsine Ait Beş Türün trnT-L-F cpDNA Bölgesi Kullanılarak Filogenetik İlişkilerinin Belirlenmesi

Ayşenur KALMER [1] , Ayten DİZKIRICI TEKPINAR [2]

196 404

The genus Centaurea L., belonging to Asteraceae family, contains systematic controversies and these taxonomic problems must be illuminated in the light of the molecular techniques developed in recent years. Especially, taxonomic position of Centaurea depressa is controversial and moving of this species to Cyanus genus is claimed by several researchers. To solve this problem, phylogenetic relationships of five species (C. depressa, C. iberica, C. solstitialis, C. virgata, C. balsamita) were tried to be determined by using the trnT(UGU) - trnL(UAA), trnL5’- trnL3’ intron and trnL(UAA) - trnF(GAA) IGS regions which are found in the chloroplast DNA and are preferred for phylogenetic studies. DNA sequences of other species belonging to the genus Centaurea were retrieved from NCBI database and analyzed to clarify the evolutionary relationships of not only C. depressa but also the other four species. Phylogenetic trees were constructed by using Maximum Likelihood (ML) and Maksimum Parsimony (MP) methods. That’s why, the data obtained from the trnL-trnF region will not be shared. Centaurea depressa is not phylogenetically separated from other related species in the trees contructed by using trnT(UGU) - trnL(UAA) and trnL5’- trnL3’ intron regions. DNA sequences obtained from trnL(UAA) - trnF(GAA) IGS region are not clear so this region is not elaborated.

Asteraceae familyasına ait Centaurea L. cinsi sistematik açıdan tartışmaları barındıran ve son yıllarda gelişen moleküler tekniklerin ışığında taksonomik problemleri aydınlatılması gereken bir cinstir. Özellikle Centaurea depressa türünün taksonomik konumu tartışmalı olup bazı araştırmacılar tarafından Cyanus cinsinde olması gerektiği iddia edilmiştir. Bu problemi açığa kavuşturmak için, cinse ait beş türün (C. depressa, C. iberica, C. solstitialis, C. virgata, C. balsamita) filogenetik ilişkileri kloroplast DNA’sında bulunan ve filogenetik çalışmalarda tercih edilen trnT(UGU) - trnL(UAA) IGS, trnL5’- trnL3’ intron ve trnL(UAA) - trnF(GAA) IGS bölgeleri kullanılarak belirlenmeye çalışılmıştır. Cinse ait bazı türlerin DNA sekansları NCBI veri tabanından alınarak analizlere eklenmiş ve hem C. depressa’nın hem de çalışılan diğer dört türün cins içindeki evrimsel ilişkileri aydınlatılmaya çalışılmıştır. Maksimum Olasılık (ML) ve Maksimum Parsimoni (MP) metodlarıyla filogenetik ağaçlar oluşturulmuştur. trnT(UGU) - trnL(UAA) ve trnL5’- trnL3’ intron bölgeleri için oluşturulan ağaçlarda C. depressa türü diğer akraba türlerden filogenetik olarak ayrılmamıştır. trnL(UAA) - trnF(GAA) IGS bölgesinde yeterli kalitede sekanslar elde edilemediğinden çalışmamızda bu bölge detaylandırılmamıştır. 

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Subjects Basic Sciences
Journal Section Natural Sciences
Authors

Author: Ayşenur KALMER

Author: Ayten DİZKIRICI TEKPINAR (Primary Author)

Bibtex @research article { csj363294, journal = {Cumhuriyet Science Journal}, issn = {2587-2680}, eissn = {2587-246X}, address = {Cumhuriyet University}, year = {2017}, volume = {38}, pages = {52 - 59}, doi = {10.17776/csj.363294}, title = {Determination of Phylogenetic Relationships of Five Species of Centaurea Genus by Using trnT-L-F cpDNA Region}, key = {cite}, author = {KALMER, Ayşenur and DİZKIRICI TEKPINAR, Ayten} }
APA KALMER, A , DİZKIRICI TEKPINAR, A . (2017). Determination of Phylogenetic Relationships of Five Species of Centaurea Genus by Using trnT-L-F cpDNA Region. Cumhuriyet Science Journal, 38 (4), 52-59. DOI: 10.17776/csj.363294
MLA KALMER, A , DİZKIRICI TEKPINAR, A . "Determination of Phylogenetic Relationships of Five Species of Centaurea Genus by Using trnT-L-F cpDNA Region". Cumhuriyet Science Journal 38 (2017): 52-59 <http://dergipark.org.tr/csj/issue/32371/363294>
Chicago KALMER, A , DİZKIRICI TEKPINAR, A . "Determination of Phylogenetic Relationships of Five Species of Centaurea Genus by Using trnT-L-F cpDNA Region". Cumhuriyet Science Journal 38 (2017): 52-59
RIS TY - JOUR T1 - Determination of Phylogenetic Relationships of Five Species of Centaurea Genus by Using trnT-L-F cpDNA Region AU - Ayşenur KALMER , Ayten DİZKIRICI TEKPINAR Y1 - 2017 PY - 2017 N1 - doi: 10.17776/csj.363294 DO - 10.17776/csj.363294 T2 - Cumhuriyet Science Journal JF - Journal JO - JOR SP - 52 EP - 59 VL - 38 IS - 4 SN - 2587-2680-2587-246X M3 - doi: 10.17776/csj.363294 UR - https://doi.org/10.17776/csj.363294 Y2 - 2017 ER -
EndNote %0 Cumhuriyet Science Journal Determination of Phylogenetic Relationships of Five Species of Centaurea Genus by Using trnT-L-F cpDNA Region %A Ayşenur KALMER , Ayten DİZKIRICI TEKPINAR %T Determination of Phylogenetic Relationships of Five Species of Centaurea Genus by Using trnT-L-F cpDNA Region %D 2017 %J Cumhuriyet Science Journal %P 2587-2680-2587-246X %V 38 %N 4 %R doi: 10.17776/csj.363294 %U 10.17776/csj.363294
ISNAD KALMER, Ayşenur , DİZKIRICI TEKPINAR, Ayten . "Determination of Phylogenetic Relationships of Five Species of Centaurea Genus by Using trnT-L-F cpDNA Region". Cumhuriyet Science Journal 38 / 4 (December 2017): 52-59. https://doi.org/10.17776/csj.363294