Determination of the Genotoxicity of the Soil in the Aydın Region Irrigated by Büyük Menderes River by the Allium Test System

Yıl 2018, Cilt: 39 Sayı: 1, 152 - 161, 16.03.2018

Zahide Şahin Serdar Koca

https://doi.org/10.17776/csj.405712

Cited By: 1

Öz

Nowadays, one of the most important problems is environmental
pollution. Environmental pollution in the Büyük Menderes River-basin, which is
one of the agricultural fields in Turkey, has reached important dimensions. B.
Menderes River which waters B. Menderes River-basin and fields around it is
contaminated by various sources.

In this study the genotoxicity of the soil
samples taken from three different regions in two different months (September
2006 and March 2007) in the Aydın region of B. Menderes River-basin was
researched with the Allium test.

The soil samples were taken from the lands
watered by the B. Menderes River and Çine Stream; the fields near the B.
Menderes Bridge (on the Muğla main road), the Çine Stream, and the Koçarlı
Bridge in September and March. 25%, 50%, 100% concentrations were prepared
diluting these samples and onions (Allium
cepa L.) were rooted. At the end of the studies, the decrease in the
mitotic index was found to be statistically important according to the control
in all the doses except 25% of the Çine doses in September and March.

The study soil samples taken from the three different
regions in two different months caused structural chromosomal aberrations such
as anaphase bridge, fragment, stickiness, and polar deviation in the Allium cepa L. root tip cells. It has
been emphasized that the difference in all the doses except for 25% Menderes,
25% Koçarlı, 50% doses in September and 25% Çine, 25% Menderes, 25%, 25%
Koçarlı, Koçarlı, 50% doses in March are important according to the control in
the total chromosomal aberrations.

Anahtar Kelimeler

Genotoxicity, Mitotic index, Chromosome aberrations, Allium test

Büyük Menderes Nehri ile Sulanan Aydın Bölgesi’ndeki Toprakların Genotoksisitesinin Allium Test Sistemi ile Belirlenmesi

Öz

Günümüzün en önemli
sorunlarından biri çevre kirliliğidir. Ülkemizin önemli tarım alanlarından biri
olan Büyük Menderes Havzasındaki çevre kirliliği önemli boyutlara ulaşmıştır.
Büyük Menderes Havzasını sulayan Büyük Menderes Nehri ve çevresindeki topraklar
çeşitli kaynaklar tarafından kirletilmektedir.

Bu çalışmada Büyük Menderes
Havzasının Aydın bölgesinde üç farklı bölgeden iki farklı ayda (Eylül 2006,
Mart 2007) alınan toprak örneklerinin genotoksisitesi Allium test ile
araştırılmıştır.

Büyük Menderes Nehri ve Çine
Çayının sularıyla sulanan topraklardan; Büyük Menderes Köprüsü (Muğla Yolu
üzeri), Çine Çayı ve Koçarlı Köprüsü yanındaki tarlalardan Eylül ve Mart
aylarında toprak örnekleri alınmıştır. Bu örnekler sulandırılarak %25, %50 ve
%100'lük konsantrasyonlar hazırlanmış ve soğanlar (Allium cepa L.) bu
sularda köklendirilmiştir.

Yapılan incelemeler sonunda
Eylül ve Mart ayları Çine %25'lik dozlar dışındaki tüm dozlarda kontrole göre
mitotik indeksteki düşüş istatistiki olarak önemli çıkmıştır. Çalışmamızda iki farklı ayda
üç farklı bölgeden alınan toprak örnekleri Allium cepa L. (soğan) kök
ucu hücrelerinde anafaz köprüsü, fragment, kromozom yapışması ve yanlış
kutuplaşma gibi yapısal kromozom anormalliklerine yol açmıştır.

Toplam kromozom aberasyonları bakımından Eylül ayı
Menderes %25, Koçarlı %25, Koçarlı %50 ve Mart ayı Çine %25, Menderes %25,
Koçarlı %25, Koçarlı %50 dozları dışındaki tüm dozlarda farkın kontrole göre
önemli olduğu belirlenmiştir.

Anahtar Kelimeler

Kromozom aberasyonları, Mitotik indeks, Allium test, Genotoksisite

Kaynakça

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