Analytical Method Validation in Forensic Assay

Year 2020, Volume: 2 Issue: 1, 9 - 24, 11.09.2020

Burcu Doğan Topal İrem Kaya Sibel A. Özkan

Abstract

Reliable analytical data is an essential requisite for accurate interpretation
of toxicological findings in the forensic science. If the analytical
data is not reliable, it may lead to wrong legal processes for the defendant
or the wrong treatment of the patient. Therefore, analytical methods
to be used in forensic science require careful method development and
validation. Method validation is the most important and key element in
establishing reference methods and evaluating the ability of a laboratory to
produce reliable analytical data. Newly developed or routine used analytical
methods must validate according to the standard guidelines. Analytical
method validation should include several performance factors such as: Selectivity,
specificity, accuracy, precision, linearity, range, limit of detection
(LOD), limit of quantification (LOQ), stability, ruggedness, and robustness
etc.

Keywords

Analytical methods, forensic science, criminal analysis, validation, determination, performance characteristics

Adli Analizde Analitik Yöntem Doğrulaması

Abstract

Adli bilimlerde toksikolojik bulguların doğru yorumlanması için güvenilir analitik verilere ihtiyaç vardır. Analitik veri güvenilir değilse, bu, sanık için yanlış hukuki süreçlere veya hastanın yanlış tedavisine yol açabilir. Bu nedenle, adli bilimlerde kullanılacak analitik yöntemlerin dikkatli bir şekilde yöntem geliştirilmesi ve doğrulanması gerekmektedir. Yöntem doğrulaması, referans yöntemlerinin oluşturulmasında ve bir laboratuvarın güvenilir analitik veri üretebilme yeteneğinin değerlendirilmesinde en önemli ve anahtar unsurdur. Yeni geliştirilmiş veya rutin olarak kullanılan analitik yöntemler, standart rehberlere göre doğrulanmalıdır. Analitik yöntem doğrulaması, seçicilik, özgüllük, doğruluk, hassasiyet, doğrusallık, aralık, tespit sınırı (LOD), nicelleme sınırı (LOQ), stabilite, sağlamlık ve robustluk gibi birkaç performans faktörünü içermelidir.

Keywords

Analitik Yöntemler, Adli Bilimler, Suç Analizi, Doğrulama, Belirleme, Performans Özellikleri

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