Chitin and Ag-decorated chitin nanocomposite obtained from Eustigmaeus absens (Acari: Stigmaeidae): Isolation, characterization and antibacterial activity

Abstract

3D chitin was obtained from the mite species Eustigmaeus absens Doğan (Acari: Stigmaeidae) by chemical method and silver nanoparticles were decorated on the obtained chitin. The resulting chitins were characterized by using attenuated total reflectance-Fourier Transform infrared spectroscopy (ATR-FTIR), scanning electron microscope (SEM), energy dispersive X-ray spectrometry (EDX) and transmission electron microscopy (TEM) and their antibacterial activities were evaluated. It was determined that the obtained chitin from E. absens contains carbon (C), nitrogen (N), oxygen (O) elements and trace amount of calcium (Ca) element. The characteristic amide-I and amide-II bands for α-chitin were observed by ATR-FTIR analysis. SEM images revealed that the surface of the chitin consists of macropores, micropores and broken nanofibers. TEM analysis showed that the sizes of silver nanoparticles differed between 6-20 nm. Images taken from the stereo microscope and the phase-contrast equipped light microscope showed that the chitin was obtained without disturbing the 3D structure of the organism. In addition, it was defined that silver nanoparticle decorated chitin exhibited antibacterial activities against the bacteria Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213.

Keywords

• Mite
• 3D chitin
• silver-chitin nanocomposite
• SEM
• TEM
• FTIR
• antibacterial activity

Eustigmaeus absens (Acari: Stigmaeidae) türünden elde edilen kitin ve Ag-dekore edilmiş kitin nanokompozit: İzolasyonu, karakterizasyonu ve antibakteriyel aktivitesi

Öz

Eustigmaeus absens Doğan (Acari: Stigmaeidae) akar türünden kimyasal yöntemle 3D kitin elde edildi ve elde edilen kitin üzerine gümüş nanoparçacıkları dekore edildi. Elde edilen kitinler seyreltilmiş toplam yansıma-fourier dönüşümlü infrared spektroskopisi (ATR-FTIR), taramalı elektron mikroskobu (SEM), enerji dağılımlı X-ışını spektrometresi (EDX) ve geçirimli elektron mikroskobu (TEM) analizleriyle karakterize edildi ve antibakteriyel aktiviteleri değerlendirildi. Elde edilen kitinin yapısında karbon (C), azot (N), oksijen (O) elementlerinin yer aldığı, eser miktarda kalsiyum (Ca) elementinin bulunduğu belirlendi. ATR-FTIR analiziyle α-kitin için karakteristik olan amid-I ve amid-II bantları gözlemlendi. SEM görüntüleri kitin yüzeyinin makro gözenekler, mikro gözenekler ve kesik nanoliflerden oluştuğunu açığa çıkardı. TEM analizleri gümüş nanoparçacıkların boyutlarının 6-20 nm arasında değiştiğini gösterdi. Stereo mikroskop ve faz-kontrast donanımlı ışık mikroskobundan alınan görüntülerle organizmanın üç boyutlu yapısını bozmadan kitinin elde edildiğini gösterdi. Ayrıca gümüş nanoparçacıklı kitinin Escherichia coli ATCC 25922 ve Staphylococcus aureus ATCC 29213 bakterilerine karşı antibakteriyel aktivite sergilediği tespit edildi.

Anahtar Kelimeler

• Akar
• 3D kitin
• gümüş-kitin nanokompozit
• SEM
• TEM
• FTIR
• antibakteriyel aktivite

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