Research Article

Isolation and characterization of 3D chitin from a mite species Trachytes pauperior (Parasitiformes: Uropodina)

Volume: 3 Number: 2 July 31, 2021
EN

Isolation and characterization of 3D chitin from a mite species Trachytes pauperior (Parasitiformes: Uropodina)

Abstract

Chitin is the most abundant structural biopolymer after cellulose in terrestrial ecosystems. Until now, chitin isolation in powder or granule form has been carried out from many macro living groups (Arthropoda, Crustacea, Mollusca etc.). However, studies on chitin characterization of microscopic organisms have remained limited. In this study, three dimensional (3D) chitin extraction was performed for the first time from Trachytes pauperior (Berlese), a mite species. The obtained chitin was observed by light microscopy and characterized by FT-IR, and SEM analysis. Our findings suggest that chitin, which was obtained in high purity and constitutes a large part of the organism's body structure, could be a potential source for future studies.

Keywords

Thanks

We would like to thank Prof. Dr. Salih DOĞAN, (Erzincan Binali Yıldırım University, Turkey) for supporting over this manuscript.

References

  1. Al Sagheer, F., Al-Sughayer, M., Muslim, S. and Elsabee, M. 2009. Extraction and characterization of chitin and chitosan from marine sources in Arabian Gulf. Carbo-hydrate Polymers, 77 (2): 410-419. doi: 10.1016/j.carbpol.2009.01.032
  2. Anitha, A., Sowmya, S., Kumar, P.S., Deepthi, S., Chennazhi, K. et al. 2014. Chitin and chitosan in selected biomedical applications. Progress in Polymer Science, 39 (9): 1644-1667. doi: 10.1016/j.progpolymsci.2014.02.008
  3. Arbia, W., Arbia, L., Adour, L. and Amrane, A. 2013. Chitin extraction from crustacean shells using biological methods–a review. Food Technology and Biotechnology, 51 (1): 12-25.
  4. Bal, D.A. 2002. Uropodid (Acari, Gamasida, Uropodina) fauna of Erzincan and Erzurum provinces. PhD Dissertation. Graduate School of Natural and Applied Sciences, Atatürk University, Erzurum, Turkey, 328 pp. [In Turkish]
  5. Bloszyk, J. 1980. Mites of the genus Trachytes Michael, 1894 (Acari, Mesostigmata) in Poland. Prace Komisji Biologicznej, Poznan, 54: 1-51.
  6. Bloszyk, J. 1999. Geographical and ecological variability of mites of the kohort Uropodina (Acari: Mesostigmata) in Poland. I. Uropodine mites of oak-hornbeam forests (Carpinion betuli). Kontekst, Poznan, Poland, 245 pp. [In Polish]
  7. Bo, M., Bavestrello, G., Kurek, D., Paasch, S., Brunner, E. et al. 2012. Isolation and identification of chitin in the black coral Parantipathes larix (Anthozoa: Cnidaria). International Journal of Biological Macromolecules, 51 (1-2): 129-137. doi: 10.1016/j.ijbiomac.2012.04.016
  8. Brimacombe, J.S. and Webber, J.M. 1964. Mucopolysaccharides: chemical structure, distribution and isolation. Elsevier, Amsterdam, The Netherlands, 181 pp.

Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Publication Date

July 31, 2021

Submission Date

April 2, 2021

Acceptance Date

May 20, 2021

Published in Issue

Year 2021 Volume: 3 Number: 2

APA
Çakmak, E., & Koç Bilican, B. (2021). Isolation and characterization of 3D chitin from a mite species Trachytes pauperior (Parasitiformes: Uropodina). Acarological Studies, 3(2), 66-72. https://doi.org/10.47121/acarolstud.908554
AMA
1.Çakmak E, Koç Bilican B. Isolation and characterization of 3D chitin from a mite species Trachytes pauperior (Parasitiformes: Uropodina). Acarol. Stud. 2021;3(2):66-72. doi:10.47121/acarolstud.908554
Chicago
Çakmak, Emel, and Behlül Koç Bilican. 2021. “Isolation and Characterization of 3D Chitin from a Mite Species Trachytes Pauperior (Parasitiformes: Uropodina)”. Acarological Studies 3 (2): 66-72. https://doi.org/10.47121/acarolstud.908554.
EndNote
Çakmak E, Koç Bilican B (July 1, 2021) Isolation and characterization of 3D chitin from a mite species Trachytes pauperior (Parasitiformes: Uropodina). Acarological Studies 3 2 66–72.
IEEE
[1]E. Çakmak and B. Koç Bilican, “Isolation and characterization of 3D chitin from a mite species Trachytes pauperior (Parasitiformes: Uropodina)”, Acarol. Stud., vol. 3, no. 2, pp. 66–72, July 2021, doi: 10.47121/acarolstud.908554.
ISNAD
Çakmak, Emel - Koç Bilican, Behlül. “Isolation and Characterization of 3D Chitin from a Mite Species Trachytes Pauperior (Parasitiformes: Uropodina)”. Acarological Studies 3/2 (July 1, 2021): 66-72. https://doi.org/10.47121/acarolstud.908554.
JAMA
1.Çakmak E, Koç Bilican B. Isolation and characterization of 3D chitin from a mite species Trachytes pauperior (Parasitiformes: Uropodina). Acarol. Stud. 2021;3:66–72.
MLA
Çakmak, Emel, and Behlül Koç Bilican. “Isolation and Characterization of 3D Chitin from a Mite Species Trachytes Pauperior (Parasitiformes: Uropodina)”. Acarological Studies, vol. 3, no. 2, July 2021, pp. 66-72, doi:10.47121/acarolstud.908554.
Vancouver
1.Emel Çakmak, Behlül Koç Bilican. Isolation and characterization of 3D chitin from a mite species Trachytes pauperior (Parasitiformes: Uropodina). Acarol. Stud. 2021 Jul. 1;3(2):66-72. doi:10.47121/acarolstud.908554

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