VCAM1 (T-1591C and T-833C) and E-selectin S128R polymorphisms are not risk factors for Hashimoto’s thyroiditis

Year 2018, , 138 - 142, 30.11.2018

Sevgin Degirmencioglu , Pervin Vural , Ayşenur Özderya Semra Dogru Abbasoglu

https://doi.org/10.25000/acem.421521

Abstract

Aim: The etiopathogenesis of Hashimoto’s thyroiditis (HT)  has not been clearly elucidated although the
role of chronical inflammation and endothelial dysfunction has been
established.   Adhesion molecules such as vascular cell adhesion molecule1
(VCAM1) and E-selectin are secreted from vascular endothelium and promote
accummulation of leukocytes in damaged endothelial areas.  This
study examined the possible association of VCAM1 (T-1591C and T-833C)
and E-selectin S128R single nucleotide polymorphisms
(SNPs) with the occurence of HT for the first time.

Methods: VCAM1 (T-1591C and T-833C), and E-selectin
S128R SNPs in DNA from peripheral blood leukocytes of 189
patients with HT and 247 healthy controls were investigated by real-time PCR
combined with melting curve analysis using fluorescence-labeled hybridization
probes.

Results: We did not find
significant differences in the distributions of studied polymorphisms   between patients with HT and healthy
controls.

Conclusions: The
results of present study suggest that VCAM1 (T-1591C and T-833C) and
E-selectin S128R SNPs may not be risk factors for HT. For all that; further
studies with a larger cohort, analyzing other polymorphisms in VCAM1 and E-selectin genes
are necessary to support our observations.

Keywords

Hashimoto’s thyroiditis, VCAM1, E-selectin, polymorphism

VCAM1 (T-1591C ve T-833C) ve E-selektin S128R polimorfizmleri Hashimoto tiroiditi için risk faktörleri değildir

Abstract

Amaç: Hashimoto tiroiditinin (HT) etyopatogenezi tam
olarak aydınlatılamamış olmakla birlikte kronik inflamasyon ve endotel
disfonksiyonunun önemli olduğu bilinmektedir. Vasküler hücre adezyon molekülü 1
(VCAM1) ve E-selektin gibi adezyon molekülleri endotel tarafından salgılanırlar
ve hasarlı endotel bölgesine lökositlerin migrasyonunu düzenlemektedirler. Bu
çalışmada ilk kez VCAM1 (T-1591C ve T-833C) ve E-selektin S128R tek nükleotid
polimorfizmleri (SNPs) ile HT arasında bir ilişki olup olmadığı
araştırılmıştır.

Metod: HT’li 189 hasta ve 247 sağlıklı kontrol kişinin
periferik kan lökositlerinden izole edilen DNA örneklerinde VCAM1  (T-1591C ve T-833C) ile E-selektin S128R
polimorfizmleri floresan boya-işaretli problar kullanan ve erime eğri analizine
dayanan “real-time” PCR yöntemi ile incelendi.

Bulgular: Çalışmamızda kontrol grubu ve HT hastalarında
araştırılan polimorfizmlerin dağılımlarında anlamlı bir fark bulunmadığı
saptandı.

Sonuç: Bu çalışmanın
bulgularına göre VCAM1 (T-1591C ve T-833C) ve E-selektin S128R polimorfizmleri
HT için bir risk faktörü olmayabileceğini düşündürmektedir. Bununla birlikte,
bulgularımızın desteklenmesi için örnek sayısının arttırılması, ayrıca VCAM1 ve
E-selektin genlerin farklı polimorfik lokuslarının da incelenmesi gerektiği
kanısındayız.

Keywords

Hashimoto tiroiditi, VCAM1, E-selektin, polimorfizm

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