Deneysel diyabetik nefropatide quercetin'in renoprotektif potansiyeli: antiapoptotik ve antioksidan etkilerin değerlendirilmesi

Year 2018, , 179 - 185, 30.11.2018

Matem Tunçdemir , Eda Büyükçolpan Mirzataş Hafize Uzun

https://doi.org/10.25000/acem.452530

Cited By: 4

Abstract

Amaç: Sıçanlarda diyabetin neden olduğu böbrek
hasarına katkıda bulunan apoptoz düzenleyici genlerin ekspresyonunu
değerlendirerek güçlü bir biyoflavonoid olan quercetin'in renoprotektif ve
antiapoptotik etkilerini araştırmak.

Yöntemler: Sıçanlar 4 gruba ayrıldı; Kontrol,
STZ-diyabetik, STZ-diyabetik + Quercetin ve Quercetin kontrol. Açlık kan
şekeri, vücut ağırlığı ve idrar mikroalbüminüri değerleri ölçüldü.
İmmünohistokimya bax, bcl-2, kaspaz-3 antikorları kullanılarak
gerçekleştirildi. Apoptoz tespiti için TUNEL yöntemi uygulandı. Homojenize
böbrek dokularında ileri oksidasyon protein ürünleri (AOPP), protein karbonil
oksidasyon (PCO), malondialdehid (MDA) ve süperoksit dismutaz (SOD) aktivitesi
ölçüldü.

Bulgular: Tedavi edilmeyen diyabetik gruba kıyasla, quercetin uygulanan
diyabetik grupta kan şekeri ve mikroalbüminüri düzeyleri anlamlı olarak
azalmıştı (sırasıyla;
p=0,020, p=0,003). MDA, AOPP ve PCO seviyeleri
anlamlı olarak azaldı (sırasıyla; p=0,001, p=0,0001, p=0,0005), ancak SOD
aktivitesinin quercetin uygulanan diyabetik grupta arttığı tespit edildi (p=0,005). Quercetin uygulanan diyabetiklerde, tedavi edilmemiş diyabetik gruba
kıyasla bcl-2, bax ve kaspaz-3'ün immün boyanması azaldı.  Tedavi edilmeyen diyabetik grubun
böbrek tübüllerinde apoptotik hücrelerde belirgin bir artış gözlenirken,
quercetin uygulanan diyabetik grupta belirgin bir düşüş gözlendi (p=0,0001).

Sonuç: Antioksidan
etkileri olan quercetin'in antiapoptotik etkilerinin, diyabetik
komplikasyonların etkilerini azaltmada ve yeni komplikasyonları önlemede
yararlı olabileceği sonucuna vardık.

Keywords

Deneysel diyabetik nefropati, Quercetin, böbrek, apoptoz, oksidatif stres

Renoprotective potential of quercetin in experimental diabetic nephropathy: assesing antiapoptotic and antioxidant effects

Abstract

Aim: We investigated renoprotective
and anti-apoptotic effects of quercetin, a potent bioflavonoid, by evaluating expression
of apoptosis-regulatory genes that contribute to the kidney damage caused by
diabetes in rats.

Methods: Rats were divided
into 4 groups; Control, STZ-induced diabetic, STZ-induced diabetic+Quercetin
and Quercetin control. Values of fasting blood glucose, body weight and urine
microalbuminuria measured. Immunohistochemistry was performed using bax, bcl-2,
caspase-3 antibodies. For apoptosis detection, TUNEL method was applied.
Advanced oxidation protein products (AOPP), protein carbonyl oxidation (PCO),
malondialdehyde (MDA) and superoxide dismutase (SOD) activity were measured in
homogenized kidney tissues.

Results: Blood glucose and
microalbuminuria levels were significantly decreased in quercetin-treated
diabetic group compared to the untreated-diabetic group (p=0.020 and p=0.003; respectively).  MDA, AOPP and PCO levels were significantly
decreased (p=0.001, p=0.0001 and p=0.0005; respectively); however, SOD activity
were found to increase in quercetin-treated diabetic group (p=0.005).
Immunostaining of bcl-2, bax and caspase-3 was decreased compared to the
untreated-diabetic group. Apoptotic cells especially increased in the kidney
tubuli of untreated-diabetic group and on the contrary, a significant decrease
was observed in the group that received a quercetin treatment (p=0.0001).

Conclusion:
Our results revealed that antiapoptotic effects of quercetin, which has predominantly
antioxidant effects, may be useful in reducing effects of diabetic
complications and preventing new complications.                                                                 

Keywords

Experimental diabetic nephropathy, Quercetin, kidney, apoptosis, oxidative stress

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