Deneysel hipertiroidizmde α-lipoik asidin oksidatif stres parametreleri üzerine etkileri

Year 2018, , 190 - 194, 30.11.2018

Adile Merve Baki Abdurrahman Fatih Aydın Pervin Vural , Merva Soluk-tekkeşin , Semra Doğru-abbasoğlu Müjdat Uysal

https://doi.org/10.25000/acem.455516

Abstract

Amaç: Deneysel hipertiroidizmde α-lipoik asid (ALA)’
in karaciğer dokusunda prooksidan-antioksidan denge üzerine etkileri ile
karaciğer fonksiyon testlerinin incelenmesi.

Yöntem: Prooksidan-antioksidan dengenin
değerlendirilmesi için, reaktif oksijen ürünleri (ROS), malondialdehit (MDA),
protein karbonil (PC), total antioksidan kapasite (FRAP)  ve glutatyon (GSH) düzeyleri ile süperoksit
dismutaz, katalaz ve glutatyon peroksidaz aktiviteleri incelendi.  Ayrıca, histopatolojik incelemeler yapıldı.
Hipertiroidi tablosu oluşturmak için T4 (12 mg/L) 10 hafta boyunca içme suyunda
uygulandı. ALA [100 mg/kg/gün; % 0.2 [w/w]; diyette] deney süresinin son 5
haftasında uygulandı.

Bulgular: Karaciğerde oksidatif stresin arttığı
görüldü. Hipertiroidili sıçanlarda ROS, MDA, PK düzeylerinde anlamlı artış
bulundu. Ayrıca FRAP düzeylerinde artış ve GSH düzeylerinde azalma görüldü. ALA
tedavisi, artan serum serbest T3 ve T4 düzeylerini düşürdü ve karaciğerde ROS,
MDA ve PK düzeylerini anlamlı olarak azalmasına neden oldu. ALA uygulaması
öncesi ve sonrasında serum karaciğer fonksiyon testlerinde bir değişiklik
görülmedi.

Sonuç: Sonuçlarımız, ALA tedavisinin
prooksidan-antioksidan dengedeki değişikliklerin düzelmesinde etkili olduğunu
ve hipertiroidi tedavisinde destekleyici ajan olarak yararlı olabileceğini
göstermektedir. 

Keywords

prooksidan-antioksidan denge, hipertiroidizm, α-lipoik asid

Effects of α-lipoic acid on oxidative stress parameters in experimental hyperthyroidism

Abstract

Aim: To investigate the effects of α-lipoic acid (ALA)
on prooxidant-antioxidant balance in liver tissue, as well as liver function
tests in experimental hyperthyroidism.

Materials and Methods: For the evaluation of
prooxidant-antioxidant balance, reactive oxygen species (ROS), malondialdehyde
(MDA), protein carbonyl (PC), ferric reducing antioxidant power (FRAP),
glutathione (GSH) levels, and superoxide dismutase, catalase and glutathione
peroxidase activities were determined. Histopathological examinations were also
performed. Hyperthyroidism was induced by the administration of L-thyroxine
[T4, 12 mg/L]  in drinking water for 10
weeks. The ALA [100 mg/kg/day; 0.2% (w/w) in diet] was administered in last 5
weeks of experimental period. 

Results: Oxidative stress in liver tissue from
hyperthyroid rats was accentuated. Significant increases in hepatic ROS, MDA,
and PC levels were found. Additionally, increased FRAP and decreased GSH levels
were observed.  ALA treatment lowered the
elevated serum free T3 and T4 levels and significantly decreased hepatic ROS,
MDA and PC levels. Serum liver function tests in hiperthyroid rats before and
after ALA treatment were not changed.

Conclusion: Our results indicate that ALA
treatment was effective in the improvement of changes in prooxidant-antioxidant
balance,  and may be useful as supportive
agent for the treatment of hypertyroidism.

Keywords

Proxidant-antioxidant balance, hyperthyroidism, α-lipoic acid

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