Timozin beta-4 A/T polimorfizmi ve akut koroner sendrom riski

Öz

Amaç: Akut koroner sendrom (ACS), koroner arter kan akımının azalması sonucu miyokard iskemisinin neden olduğu klinik tabloların tamamını ifade eder. Thymosin beta-4 geni (Tβ4) hasarlı dokuların iyileşmesinde ve ACS’de kardiyomiyositlerin canlı kalmasında önemli rol oynamaktadır. Bu çalışmada ACS’li hastaların Tβ4 genindeki A/T (rs75112573) varyasyonunun tespit edilmesi ve akut koroner hastalığına olan etkilerinin belirlenmesi amaçlanmıştır.

Yöntemler: Bu prospektif bir vaka kontrol çalışmasıdır. Çalışmaya ACS'li 48 hasta ve 45 sağlıklı kontrol birey dahil edildi. Genetik analiz, polimeraz zincir reaksiyonu/restriksiyon parça uzunluk polimorfizmi (PCR / RFLP) yöntemleri kullanılarak yapıldı.

Bulgular: Kontrol grubu ile karşılaştırıldığında hasta grubunda AT genotipi (p<0.001, X²:12.40, OR:5.42, %95 CI:2.02-14.53) ve A alleli (p<0.001, X²:17.22, OR:6.66, %95 CI:2.61-16.98) taşıma sıklığı anlamlı olarak yükselmiştir. Kontrol grubunda ise TT genotip sıklığının hasta grubuna göre istatistiksel olarak yüksek olduğu gözlemlenmiştir (p<0.001, X²:17.22, OR:2.13, %95 CI:1.44-3.16). Hasta grubumuzun LDL-kolesterol seviyesi (p<0.001, %95 CI:30.12-55.90), kontrol grubumuzun ise HDL-kolesterol seviyesi yüksek bulunmuştur (p<0.001, %95 CI:5.30-15.34). Hasta grubunda AT genotipi taşıyanlarda AA genotipi taşıyanlara göre kolesterol ve HDL-kolestrol düzeyleri anlamlı derecede yüksek olarak bulunmuştur (p=0.036, %95 CI:0.59-17.25). VLDL-kolestreol düzeyleri ise hasta grubunda AA genotipi taşıyanlarda, AT (p=0.011, %95 CI:6.73-49.86) ve TT (p=0.018, %95 CI:4.95-49.49) genotipi taşıyanlara göre anlamlı şekilde yükselmiştir.

Sonuç: Tβ4 A/T (rs75112573) gene polymorphism için AT genotipi ve A alleli taşımanın ACS riskini artırabileceği sonucuna ulaşılabilir.

Anahtar Kelimeler

• Timozin beta-4 (Tβ4) A/T,polimorfizm,akut koroner sendrom,PCR-RFLP

Thymosin beta-4 A/T polymorphism and acute coronary syndrome risk

Abstract

Aim: Acute coronary syndrome (ACS) describes all the clinical conditions due to myocardial infarction that is caused by decreased blood flow in the coronary artery. Thymosin beta-4 (Tβ4) plays a significant role in the recovery of damaged tissues and promoting the survival of cardiomyocytes in ACS. In this study, it was aimed to determine the Tβ4 A/T (rs75112573) variation in ACS and its effects on the disease.

Methods: This was a prospective case-control study. Forty-eight patients with ACS and 45 healthy controls were recruited for this study. Genetic analysis was performed using polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP).

Results: The AT genotype (p<0.001, X²:12.40, OR:5.42, 95% CI:2.02-14.53) and the A allele (p<0.001, X²:17.22, OR:6.66, 95% CI:2.61-16.98) frequency was found significantly higher in the patient group, while in the control group the TT genotype was statistically higher (p<0.001, X²:17.22, OR:2.13, 95% CI:1.44-3.16).  LDL-cholesterol levels in the patient group (p<0.001, 95% CI:30.12-55.90), and HDL-cholesterol levels in the control group (p<0.001, 95% CI:5.30-15.34) were significantly higher. In the patient group, total cholesterol and HDL-cholesterol levels were found significantly higher in AT genotype carriers compared to the AA genotype carriers (p=0.036, 95% CI:0.59-17.25), while VLDL-cholesterol levels were higher in the AA genotype carriers compared to the AT (p=0.011, 95% CI:6.73-49.86), and TT (p=0.018, 95% CI:4.95-49.49) genotype carriers.

Conclusion: It can be concluded that carrying the Tβ4 A/T (rs75112573) gene polymorphism AT genotype and the A allele may increase risk of ACS.

Keywords

• Thymosin Beta-4 A/T,polymorphism,acute coronary syndrome,PCR-RFLP

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