Thymoquinone exhibits anti-inflammatory, antioxidant, and immunomodulatory effects on allergic airway inflammation

Year 2019, , 60 - 65, 01.08.2019

Ali Parlar , Seyfullah Oktay Arslan

https://doi.org/10.25000/acem.527359

Cited By: 12

Abstract

Aim:
Asthma is an allergic disease causing mucus secretion, release various
pro-inflammatory mediators such as tumor necrosis factor- α (TNF-α) and interleukins.
The aim of this study was to evaluate the effect of thymoquinone (TQ) on
allergic airway inflammation in rats.

Methods:
Allergic airway inflammation induced by ovalbumin (OVA) challenge in
sensitized-rats and effect of TQ were studied. Inflammatory cells, interleukin
(IL)-6 and TNF-α in bronchoalveolar lavage (BAL) fluid, and lipid peroxidation (LPO) in lung tissue were measured. Microvascular
leakage was detected by Evans blue dye leakage in airway tissues.

Results:
Tidal volume was significantly lower in OVA group (1.4± 0.07 ml) than control group (1.9±0.04
ml) (p = 0.002), while breathing frequency was significantly higher in OVA
group (135.3±12.9 min^-1) than control group (p=0.017). In terms of
tidal volume, statistical significance between TQ30 and OVA groups was found (1.8±0.07
ml) (p=0.008), while in terms of breathing frequency, no significance was found
between both of them (126.7±7.3). Total white blood cell count was
significantly higher in OVA group (1,376.8±136.4 x10³/ml) than
control group (545.0±106.7 x10³/ml) (p<0.001). Statistical
significance was found in TQ10 (824.7±4.5 x10³/ml) group when
compared OVA group (p=0.036), while statistical significance was not found in
TQ1 group (1,282.2±137.7 x10³/ml). When compared OVA group (60.3±4.9
pg/ml) with control group in terms of the TNF-α level, statistical significance
was found (36.7± 4.7 pg/ml) (p=0.011). The Evans blue dye level was
significantly higher in OVA group (31.8±3.6 ng/mg of tissue) than control (12.5±1.1
ng/mg of tissue) group (p<0.001), and TQ10 group (16.3±6.7 ng/mg of tissue)
(p=0.002), and TQ30 (13.5±1.0 ng/mg of tissue) group (p<0.001).

Conclusion: These
findings reveal that TQ could be beneficial in asthma pathophysiology due to its
immunomodulatory, anti-inflammatory, and antioxidant effects.

Keywords

Allergic airway inflammation, TNF-α, microvascular leakage, ovalbumin, thymoquinone

Timokinon allerjik solunum yolu inflamasyonu üzerine anti-inflamatuar, antioksidan ve immünomodülatör etkiler gösterir

Abstract

Amaç: Astım, mukus sekresyonuna neden olan, TNF-α, IL'ler gibi çeşitli
proinflamatuar mediatörleri salgılayan alerjik bir hastalıktır. Timokinon’un
(TQ) sıçanlarda alerjik solunum yolu inflamasyonu üzerindeki etkisini
değerlendirmektir.

Yöntemler: Duyarlılaştırılmış sıçanlarda ovalbumin (OVA) ile indüklen alerjik
solunum yolu enflamasyonu ve TQ etkisi çalışıldı. Bronkoalveoler lavaj (BAL)
sıvısında enflamatuar hücre sayıları, interlökin (IL)-6 ve tümör nekroz faktör
alfa (TNF-α) düzeyleri ve akciğer dokusunda lipit peroksidasyonu (LPO)
seviyeleri ölçüldü. Mikrovasküler sızıntı, solunum yolu dokusunda Evans mavisi
ile tespit edildi.

Bulgular: Bu çalışmada, OVA grubunda tidal hacim (1,4±0,07 ml), kontrol
grubuna göre önemli ölçüde düşük bulunmuştur (1,9±0,04 ml) (p = 0,002). OVA
grubunda (135,3±12,9 dk^-1) solunum sıklığı kontrol grubuna (p=0,017)
göre anlamlı bulunmuştur. Tidal hacim açısından TQ30 (1,8±0,07 ml) grubu ile
OVA grubu karşılaştırıldığında istatistiksel anlamlılık bulunurken (p=0,008),
solunum frekansı açısından gruplar arasında istatistiksel anlamlılık
bulunamamıştır (126,7±7,3 dk^-1). OVA grubunda (1.376,8±136,4 x10³/ml) toplam beyaz küre sayısı,
kontrol grubuna göre anlamlıydı (545,0±106,7 x10³/ml) (p<0,001). TQ10 (824,7±4,5
x10³/ml) grubu ile OVA grubu
arasında istatistiksel anlamlılık bulunurken (p=0,036),  TQ1 (1.282,2±137,7 x10³/ml) ile OVA grubu arasında
istatistiksel anlamlılık yoktu. TNF-α yönünden OVA grubu (60,3±4,9 pg/ml) ile
kontrol grubu (36,7±4,7 pg/ml) karşılaştırıldığında, aralarında istatistiksel
anlamlılık bulundu (p=0,011). OVA grubunda Evans mavi düzeyi (31,8±3,6 ng/mg doku), kontrol (12,5±1,1 ng/mg doku) (p<0,001), TQ10 (16,3±6,7 ng/mg doku) (p=0,002) ve TQ30 (13,5±1,0 ng/mg doku) (p<0,001) gruplarına göre önemli derecede
yüksekti.

Sonuç: Bu bulgular, timokinonun immünomodülatör, antienflamatuar ve
antioksidan etkileri nedeniyle astım patofizyolojisinde yararlı olabileceğini
ortaya koymaktadır.

Keywords

TNF-α, Alerjik solunum yolu enflamasyonu, mikrovasküler sızıntı, ovalbumin, timokinon

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