Myeloprotective and hematoprotective role of kefir on cyclophosphamide toxicity in rats

Abstract

Aim: Kefir is a probiotic/prebiotic drink with an exotic flavor, which has been traditionally produced with fermented milk and kefir grains containing a mixture of bacteria and yeast. Cyclophosphamide (CPx), are generally limited due to numerous unwanted and severe acute toxic side-effects. For this purpose, the cell-protective effects of kefir, a natural probiotic known for its antitumor and antioxidant properties, on CPx-induced hemotoxicity and myelotoxicity were investigated. Methods: Group 1 using as control. Group 2 were administered a single dose of 150mg/kg CPx while Group 3 and 5 were given 5 and 10mg/kg kefir. Group 4 and 6 were given 5 and 10mg/kg kefir+150mg/kg CPx. While kefir was administered to the rats for 12 days, CPx was administered as single-dose on the 12th day. Results: The DPPH results show that kefir possesses high antioxidant activity. It was observed that the leukocytes, thrombocytes, erythrocytes, hemoglobin, hematocrit and bone marrow nucleated cell levels decreased in the group that was administered only CPx, and increased relatively in the groups that were administered CPx+kefir, drawing close to the control. Conclusion: The results of the present study also showed that kefir demonstrated antioxidant and cytoprotective activity, protecting blood and bone marrow cells against CPx-induced damage.

Keywords

• Kefir
• cyclophosphamide
• myelotoxicity
• hemotoxicity
• rat

Kefirin Sıçanlarda Oluşturulmuş Siklofosfamid Toksisitesi Üzerine Myeloprotektif Ve Hematoprotektif Etkileri

Abstract

Amaç: Kefir, bakteri ve maya karışımı içeren kefir taneleri ile sütten üretilen egzotik tada sahip probiyotik ve prebiyotik bir içecektir. CP gibi kanser kemoterapisi için kullanılan ilaçların kullanımı, genellikle, çok sayıda organda istenmeyen şiddetli akut toksik yan etkiler yüzünden sınırlıdır. Bu amaçla bu çalışmada antitümör ve antioksidan özellikleri iyi bilinen doğal bir probiyotik olan kefirin CP nedenli hemotoksisite ve myelotoksisite üzerine koruyucu etkileri araştırılmıştır. Gereç ve Yöntem: Grup 1(kontrol), grup 2; tek doz 150 mg/kg/b.w/i.p CP, Grup 3; 5 mg /kg / b.w kefir, grup 4; 5 mg/kg/b.w+150 mg/kg/b.w/i.p CP, grup 5; 10 mg/kg/b.w kefir, Grup 6; 10 mg /kg/b.w kefir+150 mg/kg/b.w/i.p CP. Kefir gavaj yöntemiyle 12 gün boyunca verildi. CP ise 12. Gün tek doz olarak verildi. Deneyin sonunda tüm sıçanlar disekte edilerek kan ve kemikiliği örnekleri alındı. Bulgular: Periferik kan hücreleri (lökosit, trombositler, RBC ve Hb) ve kemik iliği çekirdekli hücre düzeylerinin CP verilen grupta düştüğü, CP+kefir verilen gruplarda ise yükseldiği ve kontrole yaklaştığı görülmüştür. Sonuç: Sonuçlarımız antitümör, antioksidan ve sitoprotektif özellikleri olan kefirin kan ve kemik iliği hücrelerini CP hasarına karşı koruduğunu göstermiştir.

Keywords

• Kefir
• Siklofosfamid
• Myelotoksisite
• Hemotoksisite
• rat

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