Ratlarda Splenektomi Sonrası Red Ginsengin Lipit Profili, Endotel ve Oksidatif Hasar Üzerinde Koruyucu Etkisinin Değerlendirilmesi

Year 2021, , 43 - 49, 02.08.2021

Koray Koşmaz , Abdullah Durhan , Abdullah Şenlikci , Marlen Süleyman , Mustafa Taner Bostancı , Mevlüt Recep Pekcici , Mehmet Şeneş , İlknur Alkan Kuşabbi , Mehmet Alparslan Gönültaş , Sema Hücümenoğlu , Aziz Mutlu Barlas , Yusuf Murat Bağ , Can Ersak , Ender Ergüder

https://doi.org/10.25000/acem.952140

Cited By: 1

Abstract

Amaç: Anti-inflamatuar ve antioksidan etkili red ginsegin splenektomi sonrası görülen histopatolojik değişiklikler ve lipid profili üzerindeki etkisi araştırıldı.
Yöntemler: Sıçanlar rastgele her biri 10 sıçandan oluşan 3 gruba ayrıldı. Grup 1'de (sham), sadece laparotomi yapıldı. Grup 2'de (kontrol), splenektomi uygulandı fakat ek hiçbir tedavi uygulanmadı. Grup 3'te (Red ginseng grubu), splenektomi yapıldı ve Red Ginseng (RG) ekstresi orogastrik yoldan 100 mg / kg / gün doz olarak 28 gün süreyle uygulandı. 28 günün sonunda vasküler, intestinal ve akciğer dokularındaki histopatolojik değişiklikler incelemesi için doku örnekleri alındı ve biyokimyasal analiz için kan örnekleri alındı.
Bulgular: Total kolestrol (TC), trigliserit (TG) ve çok düşük yoğunluklu lipoprotein (VLDL) düzeyleri kontrol grubuna göre RG grubunda anlamlı olarak azalmıştır (p<0,05). Kolon ve akciğer dokusundaki mononükleer hücre infiltrasyonunun RG grubunda, kontrol grubuna göre anlamlı derecede azaldığı görüldü (p<0,05). Aort dokusunda hiç bir grup arasında fark saptanmadı. Malondialdehit (MDA) seviyelerinin de RG grubunda kontrol grubuna göre anlamlı derecede azaldığı görüldü (p<0,05). Total sülfhidril (t-SH) seviyesinin ise RG grubunda kontrol grubuna göre anlamlı derecede artış gösterdiği saptandı (p<0,05).
Sonuç: RG kullanımı splenektomi yapılan ratlarda oksidatif stresi azaltmakta ve lipid metabolizması üzerinde düzenleyici etki göstermektedir. Bununla birlikte RG kullanımının splenektomi sonrası ortaya çıkan vasküler, akciğer ve kolon epitelindeki rejenerasyonu artırıcı etkisi gözlenmemiştir.

Keywords

Splenektomi, Red ginseng, lipid profili, antioksidan

Supporting Institution

yok

Project Number

Toplantı no: 0065 proje adı: Ratlarda splenektomi sonrası Red Ginseg'in lipit profili, endotel ve oksidatif hasar üzerinde koruyucu etkisinin değerlendirilmesi Karar no: 661

Evaluation of the Protective Effect of Red Ginseng on Lipid Profile, Endothelial and Oxidative Damage after Splenectomy in Rats

Abstract

Aim: It was aimed to examine the effects of Red Ginseng (RG), which has anti-inflammatory and antioxidant properties, on the histopathological alterations and lipid metabolism following splenectomy.
Methods: Rats were randomly divided into 3 groups of 10 rats each. Only laparotomy was conducted in group 1 (sham). In group 2 (control), splenectomy was performed but no therapy was offered. Splenectomy was performed and RG extract was supplied orogastrically at a dosage of 100mg/kg/day for 28 days in group 3 (RG group). At the end of the 28-day period, tissue samples were obtained for the assessment of histopathological alterations in the vascular, intestinal, and lung tissues, and blood samples were obtained for biochemical analysis.
Results: The RG group had reduced significantly total cholesterol (TC), triglyceride (TG), and very low density lipoprotein (VLDL) levels than the control group (p<0.05). When mononuclear cell infiltration in colon and lung tissues was evaluated, it was shown to be considerably lower in the RG group (p<0.05). For aortic tissues, there was no difference between the groups. Malondialdehyde (MDA) levels were observed to be considerably lower in the RG group compared to the control group (p <0.05). The total sulfhydryl (t-SH) level increased considerably in the RG group as compared to the control group. (p<0.05)
Conclusions: After splenectomy, RG usage decreases oxidative stress in mice. It has also been demonstrated to have a regulating impact on lipid metabolism. Furthermore, the administration of RG following splenectomy was found to have a poor likelihood of improving vascular, lung, and colon epithelial regeneration.

Keywords

Splenectomy, Red ginseng, lipid profile, antioxidant

Project Number

Toplantı no: 0065 proje adı: Ratlarda splenektomi sonrası Red Ginseg'in lipit profili, endotel ve oksidatif hasar üzerinde koruyucu etkisinin değerlendirilmesi Karar no: 661

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